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UHPLC Analysis of B-vitamins on Titan™ C18 using UV detection

UHPLC Analysis of B-vitamins on Titan™ C18 using UV detection

Conditions

column TITAN C18, 50 mm x 2.1 mm I.D., x 1.9 µm particles (577122-U)
column temp. 30 C
mobile phase [A] 20 mM potassium phosphate, pH 3; [B] methanol
gradient 0.5% B held for 0.5 minutes, to 30% B in 1.3 minutes, held at 30% B for 1 minute, held at 0.5% B for 0.8 minutes
flow rate 0.5 ml/min
pressure 7977 psi (550 bar)
sample 100 ug/mL of all vitamins except 250 ug/mL of calcium pantothenate, biotin and riboflavin in water
injection 0.5 µl
detector UV, 210 nm

Description

Categories Analytical Chromatography, Pharmaceutical Analysis, Anticonvulsant/antiepiletic/antiseizure, Vitamins and Natural products
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Titan is a trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC

Materials

     
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