UHPLC Analysis of B Vitamins in Vitamin Water on Titan™ C18 using UV Detection

UHPLC Analysis of B Vitamins in Vitamin Water on Titan™ C18 using UV Detection

Conditions

column TITAN C18, 50 mm x 2.1 mm I.D., 1.9 µm particles (577122-U)
column temp. 30 C
mobile phase [A] 20 mM potassium phosphate, pH 3; [B] methanol
gradient 0.5% B held for 0.5 minutes, to 30% B in 1.3 minutes, held at 30% B for 1 minute, held at 0.5% B for 0.8 minutes
flow rate 0.5 ml/min
pressure 7977 psi (550 bar)
sample vitamin water was filtered through 0.2um nylon syringe filter
injection 0.5 µl
detector UV, 210 nm

Description

Analysis Note The vitamin water drink was filtered using 0.2 um Nylon filter and injected into UHPLC for B-vitamin analysis
Categories Analytical Chromatography, Pharmaceutical Analysis, Food & Beverage Analysis, Vitamins and Natural products, Beverage Testing, Anticonvulsant/antiepiletic/antiseizure, Sports and Energy Drinks
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Titan is a trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC

Materials

     
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