UHPLC analysis of Benzoyl peroxide and degradation products on Purospher® STAR RP-18e Hibar® HR

UHPLC analysis of Benzoyl peroxide and degradation products on Purospher® STAR RP-18e Hibar® HR

Conditions

column Purospher STAR RP-18e Hibar HR, 10 cm x 2.1 mm I.D., 2.0 μm, 120Å (1.50648.0001)
mobile phase [A] 5 mM Potassium Phosphate, pH 3.0 with phosphoric acid in water; [B] Acetonitrile
gradient 40 to 80% B in 9 min; hold at 80% B for 2 min; to 40% B in 0.1 min; to hold at 40% B for 6.9 min
flow rate 0.3 mL/min
pressure 4236 psi (292 bar)
column temp. 30 °C
detector UV, 235 nm
injection: 1 μL
sample 100 μg/mL Benzoic Acid, Ethyl Benzoate, Benzoyl Peroxide and Benzaldehyde in 30:70 acetonitrile:5mM potassium phosphate pH 3.0 in water with phosphoric acid

Description

Featured Industry Cosmetics, Personal Care, and Cleaning Products
Legal Information Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for UHPLC
Analysis Note Benzoyl peroxide is used as an acne treatment, for bleaching flour, hair and teeth, for cross-linking polyester resins, and for many other purposes. Benzoyl peroxide is split into benzoic acid and oxygen when it contacts the skin breaking down the keartin in the outermost layer, as well as aiding in the cleaning of sebum and cellular debris from pores. This helps to lower bacterial (antibacterial effect) populations in the pores on the skin. It also has a mild drying effect, which allows excess oils and dirt to be easily washed away from the skin.

Materials

     
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