UHPLC Analysis of Benzoyl peroxide from Neutrogena® Rapid Clear® stubborn acne spot gel on Purospher® STAR RP-18e Hibar® HR

UHPLC Analysis of Benzoyl peroxide from Neutrogena® Rapid Clear® stubborn acne spot gel on Purospher® STAR RP-18e Hibar® HR

Conditions

sample preparation 1 gram of Neutrogena® Rapid Clear® Stubborn Acne Spot Gel (10% benzoyl peroxide). (See "Sample Prep" at Description Text.)
column Purospher STAR RP-18e Hibar HR, 10 cm x 2.1 mm I.D., 2.0 μm, 120Å (1.50648.0001)
mobile phase [A] 5 mM Potassium Phosphate, pH 3.0 with phosphoric acid in water; [B] Acetonitrile
gradient 40 to 80% B in 9 min; hold at 80% B for 2 min; to 40% B in 0.1 min; to hold at 40% B for 6.9 min
flow rate 0.3 mL/min
pressure 4236 psi (292 bar)
column temp. 30 °C ambient
detector UV, 235 nm
injection: 1 μL
sample 100 μg/mL Benzoyl Peroxide in 30:70 acetonitrile:5mM potassium phosphate pH 3.0 in water with phosphoric acid

Description

Featured Industry Cosmetics, Personal Care, and Cleaning Products
Legal Information Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
Millex is a registered trademark of Merck KGaA, Darmstadt, Germany
Neutrogena is a registered trademark of Johnson & Johnson
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany
Rapid Clear is a registered trademark of Johnson & Johnson
suitability application for UHPLC
Analysis Note Benzoyl peroxide is split into benzoic acid and oxygen when it gets in contact with the skin breaking down the keratin in the outermost layer. This helps to lower bacterial populations in the pores on the skin. It also has a mild drying effect, which allows excess oils and dirt to be easily washed away from the skin. This application demonstrates reverse phase interactions with an improvement on the current monograph.
Sample prep 1 gram of Neutrogena® Rapid Clear® Stubborn Acne Spot Gel (10% benzoyl peroxide) was added to a 25mL volumetric flask and 10mL of acetonitrile was added by pipet. The gel and acetonitrile was vortexed for 30 seconds and sonicated for one hour. 1mL of the acetonitrile was then filled with a 0.45 μm Millex®-LH filter. 10μL of the filtered solution was added to 9.99mL of 30:70 acetonitrile:5mM potassium phosphate pH 3.0 in water with phosphoric acid to give a concentration of 100 mg/mL.

Materials

     
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