UHPLC Analysis of Bisphenol A and Analogous Compounds in Infant Formula on Titan™ C18 with Fluorescence Detection after SPE using SupelMIP® Bisphenol A

UHPLC Analysis of Bisphenol A and Analogous Compounds in Infant Forµla on Titan™ C18 with Fluorescence Detection after SPE using SupelMIP® Bisphenol A

Conditions

sample/matrix liquid infant forµla
SPE well plate SupelMIP Bisphenol A, 100 mg/3 mL (52775-U)
condition 3 mL 2% formic acid in methanol, 3 mL acetonitrile, 3 mL water
sample addition as prepared
washing 9 mL of water, 6 mL of acetonitrile:water (40:60); vacuum was pulled for 3 min after the last wash
elution 3 mL of methanol:acetonitrile (75:25) (A017)
eluate post-treatment Sample was evaporated to dryness at 45 C, reconstuituted in 0.5 mL of acetonitrile:water (40:60). All samples were filtered through Durapore membrane filter units prior to analysis.
column Titan C18, 10 cm x 2.1 mm I.D., 1.9 µm particles (577124-U)
column temp. 35 C
mobile phase [A] water; [B] acetonitrile
gradient 40 to 80% B in 2.5 min; held for 4.5 min; to 100%B in 0.1 min; held for 2.4 min; to 40% B in 0.1 min; held for 2.9 min
flow rate 0.25 ml/min for 7 min; to 0.40 ml/min in 0.1 min; held for 2.4 min; to 0.25 ml/min in 0.1 min.; held for 2.9 min
pressure 3916 psi (270 bar)
injection 5 µl
detector fluorescence, ex: 230 nm, em: 315 nm

Description

Analysis Note BPA can leach into foods from the contact between food and the packaging. Structurally analogous compounds could also be used in place of BPA during package manufacturing as BPA comes under more stringent regulations. The presented method was developed for analysis of this class of compounds and applied to infant forµla analysis. The use of molecularly imprinted polymers during cleanup produced samples that can be analysed by fluoresent detection.
Categories Analytical Chromatography, Plasticizer
Featured Industry Food and Beverages
Legal Information Titan is a trademark of Sigma-Aldrich Co. LLC, SupelMIP is a registered trademark of Sigma-Aldrich Co. LLC
Other Notes Sample Prep: 5 mL of liquid infant forµla was spiked with 10 ppb of analytes, mixed with 5.5 mL of acetonitrile, centrifuged at 4500 rpm for 10 minutes, top 3 mL were diluted with 3 mL water to load into SPE
suitability application for SPE, application for UHPLC

Materials

     
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