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UHPLC Analysis of Conjugated Estrogens on Titan™ C18

UHPLC Analysis of Conjugated Estrogens on Titan™ C18


column Titan C18, 10 cm x 2.1 mm I.D., 1.9 µm particles (577124-U)
column temp. 35 C
mobile phase [A] 10 mM potassium phosphate, pH 7.0; [B] acetonitrile; [C] methanol
gradient 90:5:5 (A:B:C) to 75:20:5 (A:B:C) in 4.35 min; to 40:45:15 (A:B:C) in 6.75 min; to 90:5:5 (A:B:C) in 7 min
flow rate 0.6 ml/min
pressure 9500 psi (655 bar)
sample 50 µg/mL each in water:acetonitrile (60:40 )
injection 2 µl
detector UV, 220 nm


Analysis Note The Titan C18 column provided efficient and rapid resolution of thirteen related estrogenic compounds. Ultra Ultra high purity solvents provided robust operation.
Categories Analytical Chromatography, Clinical, steroids, Steroids
Featured Industry Clinical
Legal Information Titan is a trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC


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