UHPLC Analysis of Conjugated Estrogens on Ascentis® Express C18

UHPLC Analysis of Conjugated Estrogens on Ascentis® Express C18

Conditions

column Ascentis Express C18, 10 cm x 2.1 mm I.D., 2 µm particles (50813-U)
column temp. 35 C
mobile phase [A] 10 mM potassium phosphate, pH 7.0; [B] acetonitrile; [C] methanol
gradient 90:5:5 (A:B:C) to 75:20:5 (A:B:C) in 4.35 min; to 40:45:15 (A:B:C) in 6.75 min; to 90:5:5 (A:B:C) in 7 min
flow rate 0.6 ml/min
pressure 9500 psi (655 bar)
sample 50 µg/mL each in water:acetonitrile 60:40
injection 2 µl
detector UV, 220 nm

Description

Analysis Note The Ascentis Express C18 column provided efficient and rapid resolution of thirteen related estrogenic compounds. Ultra high purity solvents provided robust operation.
Categories Analytical Chromatography, Clinical, steroids, Steroids
Featured Industry Clinical
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC

Materials

     
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