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UHPLC Analysis of Corticosteroids on Titan™ C18

UHPLC Analysis of Corticosteroids on Titan™ C18


column Titan C18, 10 cm x 2.1 mm I.D., 1.9 µm particles (577124-U)
column temp. 35 C
mobile phase [A] water; [B] acetonitrile; (70:30, A:B)
gradient 30% to 60% B in 3 minutes, hold for 0.2 min
flow rate 0.4 ml/min
pressure 7423 psi (512 bar)
sample 1000 ng/ml in mobile phase
injection 1 µl
detector UV, 240 nm
detector ESI (+) MS TIC SIR


Analysis Note Analysis of Corticosteroids on a Titan C18 UPLC column with MS detection in ESI (+) Mode
Categories Analytical Chromatography, Clinical, steroids, Steroids
Featured Industry Clinical
Pharmaceutical (small molecule)
Legal Information Titan is a trademark of Sigma-Aldrich Co. LLC
Other Notes Other MS Conditions:
•  Polarity ES+
•  Capillary (kV) 1.40
•  Cone (V) 54.00
•  RF (V) 2.50
•  Extractor (V) 3.00
•  Source Temperature (°C) 150
•  Desolvation Temperature (°C) 500
•  Cone Gas Flow (L/Hr) 0
•  Desolvation Gas Flow (L/Hr) 1000
•  Collision Gas Flow (ml/min) ON
•  LM 1 Resolution 13.64
•  HM 1 Resolution 13.91
•  Ion Energy 1 0.20
suitability application for HPLC


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