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UHPLC Analysis of Curcumins on Ascentis® Express RP-Amide

UHPLC Analysis of Curcumins on Ascentis® Express RP-Amide


column Ascentis Express RP-Amide, 10 cm x 4.6 mm I.D., 5 µm particles (50774-U)
column temp. 35 C
mobile phase [A] water with 0.1% formic acid; [B] acetonitrile with 0.1% formic acid;
gradient 40% B held for 1 min; 40 to 100% B in (6 min); 100% B held for 5 min
flow rate 1.0 ml/min
pressure 1270 psi (88 bar)
sample 5 curcumins 50µg/mL in 50:50, water:methanol
injection 5 µl
detector UV, 250 nm


Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC


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