UHPLC Analysis of Diazepam and Metabolites on Ascentis® Express C18

UHPLC Analysis of Diazepam and Metabolites on Ascentis® Express C18

Conditions

column Ascentis Express C18 10 cm x 2.1 mm, 2.0 µm particles (50813-U)
column temp. 35 C
mobile phase (A) water with 0.1% formic acid; (B) acetonitrile with 0.1% formic   acid ; (65:35, A:B)
flow rate 0.8 ml/min
pressure 12720 psi (877 bar)
sample 50 µg/mL in 80:20, water:methanol
injection 0.5 µl
detector UV, 240 nm

Description

Categories Analytical Chromatography, Clinical, Forensics and Toxicology, Antioxidants, anxiolytics/sedatives
Featured Industry Clinical
Forensics and Toxicology
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC

Materials

     
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