UHPLC Analysis of Flakka and Related Compounds on Ascentis® Express RP-Amide in Reversed-Phase Mode

UHPLC Analysis of Flakka and Related Compounds on Ascentis® Express RP-Amide in Reversed-Phase Mode

Conditions

column Ascentis Express RP-Amide, 10 cm x 2.1 mm I.D., 2 µm (51576-U)
column temp. 35 °C
mobile phase [A] 0.1% formic acid in water; [B] 0.1% formic acid in acetonitrile
gradient 5% to 95% A in 6 min
flow rate 0.4 mL/min
pressure 6400 psi
sample 50 µg/mL in 0.1% formic acid in water:0.1% formic acid in acetonitrile (90:10)
injection 1 µL
detector UV, 265 nm

Description

Analysis Note The rapid gradient separation of the street drug Flakka (α-PVP) and related compounds is shown here on an Ascentis Express RP-Amide column in reversed-phase mode. Highest grade UHPLC solvents were used to supply low background interference and low particulate contamination for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
Categories Analytical Chromatography, Drugs of Abuse
Featured Industry Forensics and Toxicology
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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