UHPLC Analysis of Meropenem and Biapenem Antiobiotics on Ascentis® Express OH5

UHPLC Analysis of Meropenem and Biapenem Antiobiotics on Ascentis® Express OH5

Conditions

column Ascentis Express OH5, 10 cm x 3.0 mm I.D., 2 µm particles (50967-U)
column temp. 35 °C
mobile phase [A] 100 mM ammonium formate: [B] acetonitrile: (15:85), pH to 5 with formic acid
flow rate 0.5 mL/min
pressure 1800 psi (124 bar)
sample 50 ug/mL each in 90% acetonitrile
injection 5 µL
detector UV, 290 nm

Description

Analysis Note Meropenem and biapenem are parenteral carbapenems with a broad activity spectrum against Gram-positive and Gramnegative bacteria. It is clinically important to separate and detect the meropenem and biapenem from fluids. This HPLC method rapidly resolves both compounds using HILIC chromatographic conditions.
Categories Analytical Chromatography, Antibiotics, Antimicrobials
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC

Materials

     
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