UHPLC Analysis of Protein Standard on Unix™ SEC-300: Speed Advantage

UHPLC Analysis of Protein Standard on Unix™ SEC-300: Speed Advantage

Conditions

column Sepax Zenix SEC-300, 30 cm x 4.6 mm I.D., 3.0 µm particles (Z777028)
column Sepax Unix(TM) SEC-300, 15 cm x 4.6 mm I.D., 1.8 µm particles particles
column temp. 25 C
mobile phase 0.2 M sodium phosphate, dibasic, pH 7.0 with phosphoric acid
flow rate 0.3 ml/min
pressure 1145Zenix SEC-300 (79 bar)
pressure 1660 psiUnix SEC-300 (114 bar)
sample protein standard mix
injection 1 µl (Sepax Unix)
detector UV, 215 nm
detector 2 µl (Sepax Zenix)

Description

Analysis Note A protein standard was chromatographed on a Unix SEC-300, 15 cm x 4.6 mm I.D.,1.8 μm column and a Zenix SEC-300, 30 cm x 4.6 mm I.D., 3.0 μm column to compare the speed of analysis with respect to shorter column length. In comparison to the Zenix 3 μm column, the Unix 1.8 μm column provides greater than or equal the efficiency and resolution, in half the time.
Featured Industry Life Science and Biopharma
Legal Information Unix is a trademark of Sepax Technologies
suitability application for UHPLC

Materials

     
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