UHPLC Analysis of Protein Standard on Unix™ SEC-300: Efficiency Advantage

UHPLC Analysis of Protein Standard on Unix™ SEC-300: Efficiency Advantage

Conditions

column Sepax Unix™ SEC-300, 30 cm x 4.6 mm I.D., 1.8 µm particles
column Sepax Zenix® SEC-300, 30 cm x 4.6 mm I.D., 3.0 µm particles (Z777028)
column temp. 25 °C
mobile phase 0.2 M sodium phosphate, dibasic, pH 7.0 with phosphoric acid
flow rate 0.3 mL/min
pressure 1145 psiZenix column (79 bar)
pressure 2695 psiUnix column (186 bar)
sample Protein standard mix
injection 2 µL
detector UV, 215 nm

Description

Analysis Note A protein standard was chromatographed on a Unix SEC-300, 1.8 μm column and a Zenix SEC-300, 3.0 μm column to compare the efficiency and resolution. In comparison to the Zenix 3 μm column, the Unix 1.8 μm column provides almost a two fold increase in efficiency.
Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information Unix is a trademark of Sepax Technologies, Zenix is a registered trademark of Sepax Technologies
suitability application for UHPLC

Materials

     
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