UHPLC Analysis of Protein Standard on TSKgel® UP-SW3000: Efficiency Advantage

UHPLC Analysis of Protein Standard on TSKgel® UP-SW3000: Efficiency Advantage

Conditions

column TSKgel SuperSW3000, 30 cm x 4.6 mm I.D., 4.0 µm particles (818675)
column TSKgel UP-SW3000, 30 cm x 4.6 mm I.D., 2.0 µm particles (80023448)
column temp. 25 C
mobile phase 0.2 M sodium phosphate, dibasic, pH 7.0 with phosphoric acid
flow rate 0.3 ml/min
pressure 1280 psiSuperSW3000 (88 bar)
pressure 3845 psiUP-SW3000 (265 bar)
sample Protein standard mix
injection 2 µl
detector UV, 215 nm

Description

Analysis Note A protein standard was chromatographed on a TSKgel® UP-SW3000, 2.0 μm column and a TSKgel SuperSW3000, 4.0 μm column to compare the efficiency and resolution on the smaller particle size. In comparison to the SuperSW3000 4 μm column, the UP-SW3000 2.0 μm column provides a two fold increase in efficiency.
Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information TSKgel is a registered trademark of Tosoh Corporation
suitability application for UHPLC

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