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UHPLC Analysis of Synthetic Cannabinoid Metabolites on Ascentis® Express C18

UHPLC Analysis of Synthetic Cannabinoid Metabolites on Ascentis® Express C18

Conditions

column Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.0 µm particles (50813-U)
column temp. 45 C
mobile phase [A] 0.1% formic acid; [B] 0.1% formic acid in acetonitrile; (55:45, A:B)
flow rate 0.8 ml/min
pressure 10800 psi (744 bar)
sample 50 µg/mL in 70:30, water:methanol
injection 0.5 µl
detector UV, 250 nm

Description

Analysis Note This rapid UHPLC analysis on Ascentis Express 2 μm C18 particles resolves six metabolites of synthetic cannabinoids JWH-018 and JWH-073 in less than two minutes. Synthetic cannabinoids are illicit substances that are smoked as components of herbal incense mixtures. In early 2011, the US Drug Enforcement Agency (DEA) placed several of the most popular synthetic cannabinoids, including JWH-018 and JWH-073, on their Schedule 1 list, making the possession or consumption of these compounds illegal.
Featured Industry Forensics and Toxicology
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC

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