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UHPLC analysis of Taxanes on Titan™ C18

UHPLC analysis of Taxanes on Titan™ C18


column Titan C18 10 cm x 2.1 mm, 1.9 µm (577124-U)
column temp. 40 C
mobile phase (A) water; (B) (50:50, methanol:acetonitrile)
gradient 60 to 80% B in 4.5 min; 80% B held for 1.5 min
flow rate 0.4 ml/min
pressure 6090 psi (420 bar)
sample 25 µg/mL in 70:30, water:methanol
injection 2 µl
detector UV, 227 nm


Categories Analytical Chromatography, Chemotherapy drugs
Featured Industry Pharmaceutical (small molecule)
Legal Information Titan is a trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC


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