UHPLC/MS Analysis of Anticonvulsants on Ascentis® Express HILIC

UHPLC/MS Analysis of Anticonvulsants on Ascentis® Express HILIC

Conditions

column Ascentis Express HILIC, 10 cm x 3.0 mm I.D., 2.7 µm particles (53970-U)
column temp. 35 °C
mobile phase [A] 5 mM ammonium formate pH unadjusted in 95:5, acetonitrile:water; [B] 5 mM ammonium formate pH unadjusted in 75:25, acetonitrile:water; (25:75, A:B)
flow rate 0.4 mL/min
pressure 1240 psi (85.5 bar)
sample 3 anticonvulsants 1 µg/mL in 99:1, acetonitrile:methanol
detector MS-ESI-, full scan 100-500 m/z
detector 5 µL

Description

Analysis Note The unique selectivity of the Ascentis Express HILIC phase provided rapid LC/MS resolution of these anticonvulsant compounds. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
Categories Analytical Chromatography, Pharmaceutical Analysis, Anticonvulsant/antiepiletic/antiseizure
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC-MS

Materials

     
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