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UHPLC/MS Analysis of Benzodiazepines and Glucuronide Metabolites on Titan™ C18

UHPLC/MS Analysis of Benzodiazepines and Glucuronide Metabolites on Titan™ C18

Conditions

column Titan C18 5 cm x 3.0 mm, 1.9 µm particles (577126-U)
column temp. 35 C
mobile phase [A] 0.1% formic acid in 95:5, water:acetonitrile; [B] 0.1% formic acid in 5:95, water:acetonitrile;
gradient 35 to 60% B in 1 min; held for 0.5 min
flow rate 0.6 ml/min
pressure 5800 psi (400 bar)
sample 300 ng/mL in 97:3, water:methanol
injection 2 µl
detector MS-TOF, ESI+, XIC, 100 -1000 m/z scan

Description

Analysis Note Benzodiazepines are a class of commonly prescribed psychoactive drugs. Shown here is the rapid and efficient UHPLC separation of three benzodiazepines and their glucuronide metabolites on a Titan C18 column. The highest grade solvents provided clean, robust operation. Cerilliant CRMs provided reliable quantification.
Featured Industry Clinical
Pharmaceutical (small molecule)
Legal Information Titan is a trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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