UHPLC/MS Analysis of Diazepam and Metabolites on Titan™ C18, Comparison of Flow Rate and Column Length

UHPLC/MS Analysis of Diazepam and Metabolites on Titan™ C18, Comparison of Flow Rate and Column Length

Conditions

column Titan C18, 2 cm x 2.1 mm I.D., 1.9 µm particles (577120-U)
mobile phase [A] 0.1% formic acid; [B] acetonitrile; (60:40, A:B)
flow rate various
temp. 35 C
sample 100 ng/mL in mobile phase
injection 1 µl
detector TIC MRM (UV also runs in series)

Description

Analysis Note These two sets of chromatograms show separations on 100, 50, 30, and 20 mm x 2.1 mm I.D. columns and at different flow rates and demonstrate the utility of the 1.9 μm Titan particles. The highest grade solvents provided clean, robust operation. Cerilliant CRMs provided reliable quantification.
Categories Analytical Chromatography, Forensics and Toxicology, Anticonvulsant/antiepiletic/antiseizure, Drugs of Abuse, antiepileptic/antiseizure, drugs of abuse, antidepressants, Antidepressants
Featured Industry Clinical
Forensics and Toxicology
Legal Information Titan is a trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC

Materials

     
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