UHPLC/MS Analysis of Drugs in Whole Blood on Titan™ C18 after Biocompatible Solid Phase Microextraction (BioSPME) using SPME LC Tips with C18 Chemistry

UHPLC/MS Analysis of Drugs in Whole Blood on Titan™ C18 after Biocompatible Solid Phase Microextraction (BioSPME) using SPME LC Tips with C18 °Chemistry

Conditions

sample preparation Solid Phase Microextraction
sample/matrix whole blood spiked at 10 ng/mL
desorption process methanol 30 minutes
extraction whole blood, 15 minutes
SPME fiber SPME LC Tips (57234-U)
column Titan C18, 5 cm x 2.1 mm I.D., 1.9 µm particles (577122-U)
column temp. 35 °C
mobile phase [A] 5 mM ammonium formate, pH 4; [B] 5 mM ammonium formate in acetonitrile:water (90:10, A:B)
gradient 30 to 70% B in 3 min
flow rate 0.3 mL/min
sample 3 µL
detector MS, ESI+, MRM

Description

Analysis Note Solid phase microextraction (SPME) is a viable technique for microsampling of biological matrices for subsequent HPLC, UHPLC, and LC/MS analysis. The fibers allow direct sampling without additional sample treatment. The concentration effect of the fibers provides increased analyte sensitivity compared to other techniques. Shown here is the separation of a set of drugs on a Titan C18 UHPLC column after using SPME LC tips to extract them from whole blood. The highest grade solvents provided clean, robust operation. Cerilliant CRMs provided reliable quantification.
Categories Analytical Chromatography, SPME Applications, Clinical, Forensic, Bioanalytical, Drugs & Pharmaceuticals
Featured Industry Clinical
Pharmaceutical (small molecule)
Legal Information Titan is a trademark of Sigma-Aldrich Co. LLC
suitability application for SPME, application for UHPLC

Materials

     
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