UHPLC/MS Analysis of Spice Cannabinoid Metabolites in Urine on Titan™ C18

UHPLC/MS Analysis of Spice Cannabinoid Metabolites in Urine on Titan™ C18

Conditions

sample preparation SPE (Solid Phase Extraction)
SPE well plate SupelSelect HLB 96-Well Plate, 60mg/well (575662-U)
condition 1 mL methanol, then 1 mL water
sample addition 100 ng/mL in matrix
washing 1 mL 20% methanol in water
drying none
elution 2 mL methanol
eluate post-treatment none
column Titan C18 10 cm x 2.1 mm, 1.9 µm particles (577124-U)
mobile phase [A] 0.1% formic acid in 95:5, water:acetonitrile; [B] 0.1% formic acid in 5:95, water:acetonitrile;
gradient 25% B held for 1 min; to 45% B in 0.5 min; 45% B held for 4.5 min;   to 80% B in 0.5 min; 80% B held for 4 min
flow rate 0.5 mL/min
pressure 8756 psi (604 bar)
temp. 35 °C
sample 100 ng/mL in methanol
injection 2 µL
detector MS-ESI+,100 - 1000 m/z scan, XIC

Description

Categories Analytical Chromatography, Forensics and Toxicology, Drugs of Abuse, drugs of abuse
Featured Industry Forensics and Toxicology
Legal Information Titan is a trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC

Materials

     
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