UHPLC-MS Analysis of Spice Cannabinoids and Metabolites using Titan™ C18

UHPLC-MS Analysis of Spice Cannabinoids and Metabolites using Titan™ C18

Conditions

column Titan C18 10 cm x 2.1 mm, 1.9 µm particles (577124-U)
column temp. 35 C
mobile phase [A] 0.1% formic acid in 95:5, water:acetonitrile; [B] 0.1% formic acid in 5:95, water:acetonitrile;
gradient 45% B held for 4.5 min; to 80% B in 1.5 min; 80% B held for 4 min
flow rate 0.5 ml/min
pressure 7208 psi (497 bar)
sample 14 Spice cannabinoids 300 ng/mL in 80:20, water:methanol
injection 2 µl
detector MS-ESI+,100 - 1000 m/z scan, XIC

Description

Categories Analytical Chromatography, Drugs of Abuse
Featured Industry Forensics and Toxicology
Legal Information Titan is a trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC

Materials

     
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