US EPA Method 547 (Drinking Water Glyphosate by HPLC-FL) on Supelcosil™ SAX1

US EPA Method 547 (Drinking Water Glyphosate by HPLC-FL) on Supelcosil™ SAX1

Conditions

column SUPELCOSIL SAX1, 25 cm x 4.6 mm I.D., 5 µm particles (59138)
column temp. 35 °C
mobile phase 5 mM monobasic potassium phosphate (pH 1.9 with phosphoric acid)
flow rate 0.5 mL/min
sample each analyte at 10 ug/mL in water
injection 100 µL
detector fluorescence, excitation 338 nm, emissions 455 nm

Description

Analysis Note US EPA Method 547 is used for the determination of glyphosate in drinking water by direct-aqueous injection HPLC, post-column derivatization, and
fluorescence detection. This report shows a SUPELCOSIL SAX1 as a replacement for the column listed in the method.
Categories Analytical Chromatography, Herbicides
Featured Industry Environmental
Legal Information SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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