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US EPA Method 8315 (Solid Waste Carbonyls by HPLC-UV) on Ascentis® RP Amide

US EPA Method 8315 (Solid Waste Carbonyls by HPLC-UV) on Ascentis® RP Amide

Conditions

column Ascentis RP-Amide, 25 cm x 4.6 mm I.D.,5 µm particles (565325-U)
column temp. 40 C
mobile phase B[A] DI water; [B] acetonitrile; (25:75, A:B)
mobile phase A[A] DI water; [B] acetonitrile; (60:40, A:B)
gradient 0 min 75% A; 15 min 5% A; 0 min 25% B; 15 min 95% B; A:B held for 5 min
flow rate 1.5 ml/min
sample (as listed in mobile phase)
injection 10 µl
detector UV, 360 nm

Description

Analysis Note This application demonstrates the suitability of the Ascentis RP-Amide for the analysis of 21 aldehyde/ketone DNPH derivatives.
Featured Industry Environmental
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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