USP HPLC Analysis of Ampicillin Sodium on Ascentis® Express C18

USP HPLC Analysis of Ampicillin Sodium on Ascentis® Express C18

Conditions

column Ascentis Express C18, 10 cm x 4.6 mm I.D., 5 mum particles (50536-U)
column temp. 30 °C
mobile phase [A:B:C:D] [A] acetonitrile, [B]water, [C] 1M Potassium phosphate monobasic in water, [D] 1N acetic acid in water (80:909:10:1)
flow rate 1.0 mL/min
sample 1 mg/mL in mobile phase
injection 10 µL
detector UV, 254 nm

Description

Analysis Note This application was generated by RTC on an Ascentis Express C18.
Categories Analytical Chromatography, Antibiotics, Antimicrobials
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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