USP HPLC Analysis of L-Methionine on Ascentis® Express OH5

USP HPLC Analysis of L-Methionine on Ascentis® Express OH5

Conditions

column Ascentis Express OH5 15 cm x 4.6 mm, 5 µm particles (50347-U)
column temp. 40 C
mobile phase [A] 0.005M Ammounium formate in Water (pH: 3.15 with Trifluoroacetic Acid) ), [B] Acetonitrile (30:70, A:B)
flow rate 1.0 ml/min
pressure 914 psi (63 bar)
sample 0.8 mg/mL water:acetonitrile (1:1)
injection 3 µl
detector Agilent 380-ELSD

Description

Analysis Note This USP application was generated by RTC on an Ascentis® Express OH5 column.
Categories Analytical Chromatography, Amino Acids
Featured Industry Life Science and Biopharma
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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