USP Method for the HPLC Analysis of 4-Amino-6-Chloro-1,3-Benzenedisulfonamide on Ascentis® Express C18

USP Method for the HPLC Analysis of 4-Amino-6-Chloro-1,3-Benzenedisulfonamide on Ascentis® Express C18

Conditions

column Ascentis Express C18, 5 cm x 4.6 mm I.D., 2.7 µm particles (53826-U)
column temp. 30 C
mobile phase [A] formic acid in water (5:1000); [B] acetonitrile:methanol (3:1)
gradient 3% B for 5 min; to 36% B in 14 min; to 3% B in 18 min
flow rate 1.0 ml/min
pressure 1189 psi (82 bar)
sample 0.32 mg/mL in 2.76 mg/mL sodium phosphate, monobasic (pH 2.7 with phosphoric acid):acetonitrile (7:3)
injection 6 µl
detector UV, 275 nm

Description

Categories Analytical Chromatography, Diuretics
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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