Analytical Method: Calcium in plant material

Instruments and Reagents:

Cat.No. 173016  Spectroquant® VIS Spectrophotometer Prove 100 or

Cat.No. 173017  Spectroquant® UV/VIS Spectrophotometer Prove 300 or

Cat.No. 173018  Spectroquant® UV/VIS Spectrophotometer Prove 600 or

Cat.No. 109748  Spectroquant® Photometer NOVA 30 or

Cat.No. 109751  Spectroquant® Photometer NOVA 60 or

Cat.No. 173632  Spectroquant® Colorimeter Move 100

Cat.No. 114946  Rectangular cells 10 mm and/or

Cat.No. 114947  Rectangular cells 20 mm and/or

Cat.No. 114944  Rectangular cells 50 mm
Cat.No. 114815  Spectroquant® Calcium Test
Cat.No. 109057  Hydrochloric acid 1 mol/l
Cat.No. 116754  Water for analysis
Cat.No. 114724  Empty cells 16 mm with screw caps
Thermoreactor
 
Sample preparation:
In a closed empty cell mix 50 mg of the dried plant material with 5 ml hydrochloric acid 1 mol/l and heat at the thermoreactor at 120 °C (alternatively 100 °C) for 60 minutes.
Transfer the cooled solution into a 25-ml volumetric flask, fill up to the volume with water for analysis and mix well. Should the solution appear turbid, filter before analysis.
 
Analysis:
Determine with the above mentioned test kit.
 
Calculation:

Calcium content in %  =   analysis value in mg/l Ca x 2.5
weight of sample in mg

 

 

 

Materials

     
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