Analytical Method: Perchlorate in wastewater

Reagents and accessories:
Cat.No. 102445  Chloroform for analysis
Cat.No. 159270  Methylene blue
Cat.No. 116754  Water for analysis
Cat.No. 114724  Empty cells 16 mm with screw caps
Separating funnels with ground glass stoppers 100 ml
Membrane filter 0.45 μm pore diameter
 
Reagent 1:
Dissolve 0.10 g methylene blue in 100 ml of water for analysis.
 
Measurement range:
1.0 - 10. 0 mg/l ClO4 at cell length 16 mm and 665/660 nm
 
Sample preparation:
The sample solution must be clear; if not, filter through a folded filter or membrane filter.
 
Analysis
:
Mix 10 ml clear sample, 0.20 ml reagent 1 and 8.0 ml chloroform in a separating funnel. Close off the funnel and shake for 30 seconds. Allow to settle for 15 minutes and then separate off the lower organic phase through cotton wool directly into a 16-mm round cell with screw cap. Close off the cell. Measure at 665 nm (660 nm in the case of the SQ 118 photometer against pure chloroform).
 

Parameter list for programming the photometer Spectroquant® NOVA 60 und NOVA 400

 

Method number ----
Method-designation ClO4
Wavelength 665 nm
Citation ClO4
Units mg/l
Resolution 0.1
Zero point (E0) 0.000
Slope (E/C) 0.115
Meas. range begin 1.0
Meas. range end 10.0
Reference cell 16 mm round

 
Calculation when measuring the absorbance value using the above NOVA photometers without programming the method in the memory of the instrument:

 

Measured absorbance
of samples in A
Result in mg/l
[A sample * 1 / 0.115]
1.000 8.7

 

Parameter list for programming the photometer SQ 118

 

01 Method ClO4
02 Cat.No. -------
03 Measuring range 1.0 - 10.0
04 Units mg/l
05 Reaction time 15 0 min
06 Cell 16 mm round
07 Wavelength 660 nm
08 Calibration Factor
09 Evaluation Linear
10 Factor 8.73

 
Calculation when measuring the absorbance value using the photometer SQ118 without programming the method in the memory of the instrument:

 

Measured absorbance
of samples in A
Result in mg/l
[A sample * 8.73]
1.000 8.73

 

Materials

     
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