TLC Analysis of Alkaloids by Ion-Pair Chromatography on HPTLC Silica Gel 60 RP-8 F254s

TLC Analysis of Alkaloids by Ion-Pair Chromatography on HPTLC Silica Gel 60 RP-8 F254s

Conditions

detector in situ evaluation with TLC/HPTLC scanner (Camag) UV 254 nmdipped
in dichlorofluorescein (0.2% in ethanol)
plate TLC pre-coated plate RP-8 F 254s (1.15389.0001, 1.15423.0001)
migration distance 7 cm
eluent [A] methanol; [B] aqueous solution of 0.05 M; KH2SO4 pH4; (40:60, A:B)
and 0.1 M octane-l-sodium sulfonate
chamber normal chamber without chamber saturation

Description

Categories Analytical Chromatography, TLC Applications, alkaloids
Featured Industry Pharmaceutical (small molecule)
suitability application for TLC

Materials

     
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