TLC Analysis of Cholesterol and its Bile Acid Metabolites on HPTLC Pre-Coated Plate RP-18 F254s

TLC Analysis of Cholesterol and its Bile Acid Metabolites on HPTLC Pre-Coated Plate RP-18 F254s

Conditions

sample 0.2 µL
detector in situ evaluation with TLC/HPTLC scanner (Camag) UV 254 nm
dipped in dichlorofluorescein (0.2% in ethanol)
detector in situ evaluation with TLC/HPTLC scanner (Camag) UV 238 nm
eluent [A] petroleum benzene (40-60 degrees); [B] acetone; (80:20, A:B)
migration distance 7 cm
chamber normal chamber without chamber saturation
plate HPTLC pre-coated plate DIOL F 254s ()

Description

Categories Analytical Chromatography, TLC Applications, peptides
Featured Industry Environmental
General description TLC Plate Information: 1.05636.0001
suitability application for TLC

Materials

     
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