TLC Analysis of Hormones on HPTLC Silica Gel 60 RP-18 wF 254s with Concentrating Zone

TLC Analysis of Hormones on HPTLC Silica Gel 60 RP-18 wF 254s with Concentrating Zone

Conditions

sample 5 µL
migration distance 5 cm
chamber normal chamber without chamber saturation
plate HPTLC pre-coated plate RP-18 wF 254s with concentrating zone (1.13124.0001)
detector
Dipped in MnCl2-sulfuric acid heated up to 110 °C for 10 min in situ evaluation
(with TLC/HPTLC scanner (Camag) UV 366 nm)
eluent [A] acetonitrile; [B] water; (60:40, A:B)

Description

Categories Analytical Chromatography, TLC Applications, peptides
Featured Industry Life Science and Biopharma
suitability application for TLC

Materials

     
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