Genome-wide Collection of Lenti 3’UTR Reporters for Functional Validation of microRNA Targets
Sigma® Life Science and SwitchGear Genomics™
microRNAs (miRNAs) are a class of naturally occurring small non-coding RNA molecules that regulate a variety of developmental and physiological processes. To better understand miRNA-UTR interactions, Sigma Life Science has partnered with SwitchGear Genomics to create a genome-wide collection of 10,000 3’ UTR lenti-based luciferase reporters. This collection can be used to validate miRNA targets identified by prediction algorithms or other experimental methodologies.
Here we present data on a screen of a large set of predicted miR-122 targets using 3’ UTR luciferase GoClone™ reporters and validation of several known mRNA targets of human miRNAs using the MISSION® 3’UTR Lenti Goclones and microRNA mimics.
Repression of Lenti GoClone™ Activity Using miRNA Mimics with Published Human miRNA Targets
Stable Cell Line Creation with MISSION 3’UTR Lenti GoClone Workflow —a Renewable Resource for miRNA Validation
MISSION 3’UTR Lenti GoClones Respond Specifically to hsa-miR-122 Validated Gene Targets
- Validated miRNA target knockdown in both plasmid and lentiviral based UTR reporter constructs
- MISSION 3’ Lenti GoClones allow integration into cell lines recalcitrant to transfection and the creation of stable 3’UTR reporter cell lines
- Genome-wide pre-cloned 3’UTR sequences provide researchers a measurable savings in both time and money
|HMC0002||MISSION® LightSwitch Luciferase Assay Reagent™|
|HMI1002||MISSION® 3′UTR Lenti GoClone™ Powered by SwitchGear Genomics™, 3′UTR, human, GAPDH|
|HUTR01430||MISSION® microRNA Mimic hsa-miR-122|
|MLS0001||MISSION® miRNA, Negative Control 1 Sequence from Arabidopsis thaliana with no homology to human gene sequences|
©2012 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA and SIGMA-ALDRICH are trademarks of Sigma-Aldrich Co. LLC, registered in the US and other countries. MISSION is a registered trademark of Sigma-Aldrich Co. LLC. Where Bio Begins is a trademark of Sigma-Aldrich Co. LLC. SwitchGear Genomics, LightSwitch and GoClone are trademarks of SwitchGear Genomics. SpectraMax is a registered trademark of Molecular Devices Corp.