Fibronectin

By: George Sitterley, BioFiles 2008, 3.8, 8.

Fibronectin (FN) is a multifunctional, extracellular matrix glycoprotein composed of two nearly identical disulfidebound polypeptides of molecular weight 220 kDa. Cellular fibronectin is structurally and antigenically similar to cold insoluble globulin from plasma, therefore polyclonal antibodies to either form usually crossreact. Careful analysis of the fibronectin molecule indicate that it contains several functionally and structurally distinct domains which may bind to cell surfaces, collagen, fibrinogen or fibrin, complement, glycosaminoglycans, proteoglycans and heparin. Numerous studies have shown that fibronectin may enhance cell adhesion and spreading and affect the routes of cell migration both in vivo and in culture. Moreover, it has been shown that upon malignant transformation many cells lose most of their surface bound fibronectin. Fibronectin has been shown to also play a role in cellular morphology, cytoskeletal organization, phagocytosis, hemostasis, embryonic differentiation and wound repair. Fibronectin is produced by a wide variety of epithelial and mesenchymal cells in vitro including: fibroblasts, chondrocytes, myoblasts, Schwann cells, macrophages, hepatocytes and intestinal epithelial cells. Cellular fibronectin is present in many tissues including spleen, lymph node, tonsil, blood vessel walls, liver, kidney, muscle, skin, brain and peripheral nerves. It is found in basement membranes and in loose connective tissue stroma. It is also present in platelet α-granules and is expressed on the platelet surface after activation.

Fibronectin (FN) is a multifunctional, extracellular matrix glycoprotein composed of two nearly identical disulfidebound polypeptides of molecular weight 220 kDa.


Fibronectins

Name Source Storage Temp Target Cells For Attachment Concentration For Use Cat. No.
Fibronectin, solution from bovine plasma

2-8°C

Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells

1 ‑ 5 μg/cm2

F1141-1M
F1141-2MG
F1141-5MG
Fibronectin, powder from rat plasma

−20°C

Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells

1 ‑ 5 μg/cm2

F0635-.5MG F0635-1MG F0635-2MG
Superfibronectin, solution from human plasma and Escherichia coli

2-8°C

Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells

1 μg/cm2

S5171-.5MG
Fibronectin Fragment III1-C, lyophilized powder Escherichia coli

−20°C

Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells

0.45 μg/cm2

F3542-.5MG
Fibronectin Adhesionpromoting Peptide

-

−20°C

-

-

F3667-1MG
F3667-5MG
Fibronectin-like Protein Polymer, lyophilized powder genetically engineered

-

Recommended as a cell culture substratum at 2-10 μg/cm2.

-

F5022-1MG
F5022-5MG
Fibronectin, lyophilized powder from bovine plasma

−20°C

Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells

1 ‑ 5 μg/cm2

F4759-1MG
F4759-2MG
F4759-5MG
Fibronectin, lyophilized powder from human foreskin fibroblasts

−20°C

Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells

1 ‑ 5 μg/cm2

F2518-.5MG
Fibronectin, lyophilized powder from human plasma

−20°C

Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells

1 ‑ 5 μg/cm2

F2006-1MG
F2006-2MG
F2006-5MG
Fibronectin, liquid from human plasma

2-8°C

Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells

1 ‑ 5 μg/cm2

F0895-1MG
F0895-2MG
F0895-5MG
Fibronectin-like Engineered Protein Polymer-plus, powder genetically engineered

-

Recommended for use as a cell culture substratum at 2-10 μg/cm2.

-

F8141 -1MG

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