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Microbiological Media and Detection

By: Chloe McClanahan, BioFiles Edition 6.2

HybriScan™ Detection

Molecular biology-based methods, like PCR, can be used for lactobacilli detection. However, they are often quite expensive. Sigma-Aldrich provides a revolutionary molecular biology method that is rapid, easy, and cost effective. Based on the detection of rRNA, this method completely avoids the need for PCR amplification. The sandwich hybridization test, called HybriScan, is performed on a microtiter plate. The range of lactobacilli detected by HybriScan tests is listed in the table. For more information about the test and the technical principles, please visit sigma-aldrich.com/hybriscan.

 

Name Application Reactions Cat. No.
HybriScan D Beer Detection of all relevant beer spoilage organisms of genus Lactobacillus, Pediococcus, Pectinatus and Megasphaera 96 reactions 62533
HybriScan D Drinks Detection of bacteria and yeasts in fruit juices and nonalcoholic beverage; determination of total viable count 96 reactions 68301
HybriScan D Lactobac Detection of Lactobacilli in fruit juices and non-alcoholic beverage. 96 reactions 59744
HybriScan I Lactobacillus brevis Identification of Lactobacillus brevis 48 reactions 75724
HybriScan I Lactobacillus buchneri Identification of Lactobacillus buchneri 48 reactions 80065
HybriScanI Lactobacillus lindneri Identification of Lactobacillus lindneri 48 reactions 86827

 

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Materials

Selective Media for Bifidobacteria

Name Application Cat. No.
BSM Agar For the selective isolation and identification of Bifidobacteria.
The medium is used for quality control in the manufacture of dairy products.
88517
BSM Broth For the selective isolation and identification of Bifidobacteria.
The medium is used for quality control in the manufacture of dairy products.
90273
BSM Supplement An antimicrobial supplement recommended for the selective isolation of Bifidobacteria. 83055
Wilkins Chalgren anaerobic agar Used for the isolation of anaerobic bacteria. W1761

 

Differentiation Media for Lactobacilli

Name Application Cat. No.
Litmus Milk For maintenance of Lactobacilli and for determining the action of bacteria on milk. 17158
LS Diff erential Agar For the maximum growth and differentiation of Lactobacilli and Streptococci on the basis of colonial morphology, 2,3,5-triphenyltetrazolium chloride reduction, and casein reaction. 17153
WL Nutrient Agar For the examination of materials encountered in brewing and for industrial fermentations containing mixed fl ora of yeasts and bacteria. 17222
WL nutrient broth Recommended for the cultivation of bacteria encountered in breweries and industrial fermentations. W2261

 

Nonselective Media for Lactobacilli

Name Application Cat. No.
Elliker Broth For culturing Streptococci and Lactobacilli of importance in the dairy industry. 17123
Plate Count Skim Milk Agar For the enumeration of bacteria in milk and dairy products. 80957
Tomato Juice Agar For the cultivation and enumeration of Lactobacilli. 17216
Tomato Juice Broth For cultivation of yeasts and other aciduric microorganisms. 17218
Tryptone Glucose Yeast Extract Agar Recommended for enumeration of bacteria in water, air, milk and dairy products. T2188
Yeast malt agar Used for the isolation and cultivation of yeasts, molds and other aciduric microorganisms. Y3127

 

Selective Media (by low pH and selective agents) for Lactobacilli

Name Application Cat. No.
Lactobacillus bulgaricus Agar (Base) Used with acetate buff er for isolation and identifi cation of Lactobacillus bulgaricus. 17154
MRS Agar For the enrichment, cultivation and isolation of all species of Lactobacillus from all types of material according to DeMan, Rogosa, and Sharpe. 69964
MRS Agar, original acc. DeMan-Rogosa-Sharpe For the enrichment, cultivation and isolation of all species of Lactobacillus from all types of materials. Recommended by the "Schweizerisches Lebensmittelbuch" 5th ed., chapter 56A. 30912
MRS Agar, Vegitone This MRS Agar is free of animal derived material. It is recommended for the isolation and cultivation of Lactobacillus species. 41782
MRS Broth For the enrichment and isolation of all species of Lactobacilli from all types of material. 69966
MRS Broth modifi ed, Vegitone This MRS Broth contains plant peptone instead of animal peptone. It is recommended for the isolation and cultivation of Lactobacillus species. 38944
NBB Agar Base, modified Selective medium used for the detection of contaminating/spoilage microorganisms in brewery. 64198
NBB Broth Base, modified Selective medium used for the detection of contaminating/spoilage microorganisms in brewery. 50725
Orange-serum Agar For the isolation, cultivation and enumeration of acid-tolerant spoilage microorganisms in fruit juice and fruit juice concentrates, in particular from citrus fruit, according to Hays, Troy, and Beisel. The prepared agar may be turbid after autoclaving due to orange extract. 75405
Raka-Ray Agar A medium for selective isolation of lactic acid bacteria from beer and brewing processes 02538
Rogosa SL agar Used as a selective medium for cultivation of Lactobacilli. R1148
Sorbic acid Agar (Base) For the isolation and differentiation of Lactobacilli from food, feces, etc. according to Reuter. 85515
Universal Beer Agar For culturing microorganisms of signifi cance in the brewing industry. 17226
WL Diff erential Agar For selective isolation and enumeration of bacteria encountered in breweries and industrial fermentations. 17215

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