Poly-L-Lysine Cell Attachment Protocol

By: George Sitterley, BioFiles 2008, 3.8, 12.

Poly-Lysine (Cat. Nos. P7280, P6407, P7405, P9155, P6282, P5899, P4707*, P4832*)

Optimal conditions must be determined for each cell line and application.

  1. Add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine.
  2. Aseptically coat culture surface with 1.0 mL/25 cm2 (only). Rock gently to ensure even coating of the culture surface.
  3. After 5 minutes, remove solution by aspiration and thoroughly rinse surface with sterile tissue culture grade water.
  4. Allow to dry at least 2 hours before introducing cells and medium.

*NOTE: Step 1 is not necessary for Poly-lysine Solution, (Cat. Nos. P4707 and P4832).

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