Poly-lysine

By: George Sitterley, BioFiles 2008, 3.8, 12.

Polyamino acids facilitate the attachment of cells and proteins to solid surfaces in biological applications. In cell cultures normal attachment, growth, and development of many cell types are dependent on attachment factors and extracellular matrix components. While some cells are able to synthesize these components, others require an exogenous source, particularly when grown in serum-free culture. To help facilitate attachment, cell spreading, growth, morphology, differentiation, and motility of your cells, Sigma® offers a number of sterile, cell culture tested polyamino acids. In histochemical applications both polymers of D- and L-lysine are used to coat slides to promote attachment of cells. Poly-L-lysine has also been reported to improve protein coating of ELISA plates.

Sigma offers both Poly-D-Lysine and Poly-L-Lysine in several molecular weight ranges. Poly-Lysine enhances electrostatic interaction between negatively-charged ions of the cell membrane and positively-charged surface ions of attachment factors on the culture surface. When adsorbed to the culture surface, it increases the number of positively-charged sites available for cell binding.

Poly-L-Lysine is a charge enhancer; therefore, it can be used for coating many surfaces.

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Poly-lysines

Name Form Molecular Weight Conc. Storage Temp Application Cat. No.
Poly-L-lysine sterile-filtered 70,000‑150,000

0.01%

2-8°C

Recommended as a cell culture substratum when using 0.5 mL of a 0.1 mg/ml solution to coat 25 cm2. Optimal conditions for attachment must be determined for each cell line and application. P4707-50ML
Poly-Lornithine sterile-filtered sterile-filtered 30,000‑70,000

0.01%

2-8°C

Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm2. P4957-50ML
Poly-L-lysine sterile-filtered 150,000‑300,000

0.01%

2-8°C

Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm2. Optimal conditions for attachment must be determined for each cell line and application. P4832-50ML
Poly-D-lysine lyophilized powder, γ-irradiated average mol wt 30,000‑70,000

-

−20°C

Poly-lysine with mol. wt. >30,000 is useful in promoting cell adhesion to solid substrates P7280-5MG
Poly-L-lysine lyophilized powder, γ-irradiated 30,000‑70,000

-

−20°C

Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm2. Optimal conditions for attachment must be determined for each cell line and application. P9155-5MG
Poly-D-lysine lyophilized powder, γ-irradiated 70,000‑150,000

-

−20°C

Poly-lysine with mol. wt. >30,000 is useful in promoting cell adhesion to solid substrates. P6407-5MG
Poly-L-lysine lyophilized powder, γ-irradiated 70,000‑150,000

-

−20°C

Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm2. Optimal conditions for attachment must be determined for each cell line and application. P6282-5MG
Poly-D-lysine lyophilized powder, γ-irradiated >300,000

-

−20°C

Poly-lysine with mol. wt. >30,000 is useful in promoting cell adhesion to solid substrates. P7405-5MG
Poly-L-lysine lyophilized powder, γ-irradiated ≥300,000

-

−20°C

Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm2. Optimal conditions for attachment must be determined for each cell line and application. P5899-5MG

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