Fibronectin Cell Attachment Protocol

By: George Sitterley, BioFiles 2008, 3.8, 9.

Fibronectin Protocol

Fibronectin (Cat. Nos. F1141*, F0895*, F4759, F2006, F0635)

Optimal conditions for attachment must be determined for each cell line and application.

  1. Reconstitute with 1 mL sterile H2O/mg of protein. Allow to dissolve for at least 30 minutes at 37°C. A small amount of undissolved material may remain. This will not affect product performance.
  2. Dilute fibronectin in sterile balanced salt solution and coat the culture surface with a minimal volume.
  3. Allow to air dry for at least 45 minutes at room temperature. Excess fibronectin may be removed by aspiration, but is not necessary.

*Note: Step 1 is not necessary for the Fibronectin Solutions, (Cat. Nos. F1141 and F0895).

Freezing and thawing of reconstituted fibronectin is not recommended as breakdown of protein will occur.

Cellular Fobronectin Protocol

Cellular Fibronectin (Cat. No. F2518)

Optimal conditions for attachment must be determined for each cell line and application.

  1. Reconstitute with 0.5 mL sterile water. Do not agitate. Allow the solution to stand 30 minutes to solubilize. A small amount of undissolved material may remain. This will not affect product performance.
  2. Dilute fibronectin in sterile balanced salt solution and coat the culture surface with a minimal volume.
  3. Allow to air dry for at least 45 minutes at room temperature. Excess fibronectin may be removed by aspiration, but is not necessary.

Ask a Scientist!

Cat. No. F1141:

(Fibronectin from bovine plasma)

  1. How to choose which fibronectin product to use to coat culture surfaces for cell growth?
    The species source for the fibronectin used when coating culture surfaces is not important. It is the presence of the RGD binding site that is present in all fibronectins that promotes binding.

  2. How long can I store the fibronectin solution?
    The product is good for at least a year at 2-8°C if kept sterile.

  3. How long can I store fibronectin coated plates, etc?
    Fibronectin coated cultureware can be stored for 2-4 weeks at 2-8°C in a closed sterile container or sterile sealable bags.

Fibronectin Proteolytic Fragments

Fibronectin fragments are obtained from plasma fibronectin by using proteolytic enzymes. These fragments are useful tools for mapping regions within the fibronection molecule, which are responsible for specific binding to collagen and for other biologically relevant functions.

Product List

Name Form Source Storage Temp Binding Activity Cat. No.
Fibronectin Proteolytic Fragment lyophilized powder from human plasma −20°C Gelatin binding fragment. F0162
Fibronectin Proteolytic Fragment lyophilized powder from human plasma −20°C Heparin and gelatin binding fragment. F0287
Fibronectin Proteolytic Fragment lyophilized powder from human plasma −20°C Heparin binding fragment. F9911

Materials

     
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