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Vitronectin

By: George Sitterley, BioFiles 2008, 3.8, 13.

BioFiles 2008, 3.8, 13.

Vitronectin is a glycoprotein present in plasma and tissues.1,2 Vitronectin is implicated in various biological processes. Together with fibronectin, vitronectin is one of the major cell adhesion proteins in plasma. Although these proteins have similar functions and have an Arg-Gly-Asp cell recognition sequence, they are structurally and immunologically distinct. In addition to promoting the adhesion of various cells in culture, vitronectin binds to glycosaminoglycans, is incorporated as an inhibitor to the membrane cytolytic attack complex of the complement system, interacts with thrombin and antithrombin III during coagulation, and may have a physiological role in the coagulation pathway.3

The N-terminal residues of VTN are identical to somatomedin B, which is also present in plasma. This sequence is followed by an R-G-D sequence which interacts with a specific cell-surface receptor. Then a sequence of repeat units follows. The central domain of the molecule is enriched with hydrophobic residues. Near the C-terminus is a 12 kDa, arginine rich region responsible for heparin binding activity, expressed after a conformational change. The conformational change occurs in vivo upon binding to the thrombinantithrombin III complex and in vitro with treatment with urea.3,4

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Vitronectins

Name Source Storage Temp Target Cells For Attachment Concentration For Use Cat. No.
Vitronectin, lyophilized powder from bovine plasma

2-8°C

Cells with integrin receptors that bind vitronectin; platelets, endothelial cells, melanoma cells, osteosarcoma

0.1 μg/cm2

V9881-50UG
Vitronectin, lyophilized powder from human plasma

2-8°C

Cells with integrin receptors that bind vitronectin; platelets, endothelial cells, melanoma cells, osteosarcoma

0.1 μg/cm2

V8379-50UG
Vitronectin, lyophilized powder from rat plasma

2-8°C

Cells with integrin receptors that bind vitronectin; platelets, endothelial cells, melanoma cells, osteosarcoma

0.1 μg/cm2

V0132-50UG

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Materials

     

References

  1. Shaffer, M.C. et al., J. Lab. Clin. Med., 103, 783 (1984).
  2. Conlan, S.V. et al., Blood, 72, 185 (1988).
  3. Preissner, K.T., Annu. Rev. Cell Biol., 7, 275 (1991).
  4. Preissner, K.T., and Seiffert, D., Thrombosis Research, 89, 1-21 (1998).

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