Custom RNA Oligos

RNA Oligo Specifications

Synthesis Scales 0.05, 0.2, and 1.0 µmole
mg - gram quantity manufacturing available upon request
Purification Desalt, HPLC, PAGE
Purification is recommended for oligos > 30 bases
Length 7 – 45 bases
For RNA oligos over 45 bases, contact us for feasibility
Backbone Phosphodiester / Phosphorothioate
Format Supplied Dry
Modifications/Bases Variety of modification options including Amines, Biotins, Fluorescent Dyes, and Phosphorylation
Mixed RNA/DNA/2-OMe
Packaging 2 mL Tubes
Quality Controls MALDI-TOF MS, Electrospray Ionization MS, OD by UV Spectroscopy
Technical Datasheet & Labels Includes yield, Tm (melting temperature), MW, and µg/OD
Duplicate set of labels with each order
Additional Services Aliquoting into tubes
MSQC traces available upon request

Shipping Schedule

Scale (µmole) Desalt HPLC PAGE
0.05, 0.2, and 1.0 5 days 6 days 8 days

Minimum Yield Guarantees

Non-Modified RNA Oligos (O.D./µg)*
Scale (µmole) Desalt HPLC PAGE
0.05 5/150 1/30 0.5/15
0.2 12/360 2.5/75 1/30
1.0 40/1,200 13/390 5/150

*Guarantees are for 20 mers or longer. Oligos shorter than 20 bases will have proportionally fewer ODs.  Microgram amounts are calculated based on a 30 μg/OD value.  If the μg/OD value is less than 30, the reported μg value will be proportionally less. Phosphorothioated yields are 70% of the above stated values.

Modified RNA Oligos (O.D./µg)*
Scale (µmole) Desalt HPLC PAGE
0.05 2/60 0.4/12 0.2/6
0.2 5/150 1/30 0.4/12
1.0 16/480 5/150 2/60

*Guarantees are for 20 mers or longer. Oligos shorter than 20 bases will have proportionally fewer ODs.    Microgram amounts are calculated based on a 30 μg/OD value.  If the μg/OD value is less than 30, the reported μg value will be proportionally less. Phosphorothioated yields are 70% of the above stated values.  Post-synthesis modifications may yield 50% less than the above stated values.

Materials