Extract-N-Amp™ Product Guide

Your shortcut for PCR

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays. Eliminate the need for any columns or long enzymatic sample purification with this simple “lyse & go” method for PCR. Kits include all reagents, proprietary buffers, plus enzymes needed to rapidly extract and amplify targets of interest from a variety of cells and tissues.

  • Integrated purification and amplification
  • Simplified sample processing


Watch the full video protocol here

The kits provide all the reagents necessary to rapidly extract and amplify DNA targets of interest by PCR. A novel extraction method eliminates the need for long enzymatic digestions or homogenization. The kit also includes a specially formulated hot start PCR ReadyMix™ for amplification directly from the extract. The PCR ReadyMix comes in two formulations: Extract-N-Amp PCR ReadyMix and REDExtract-N-Amp PCR ReadyMix. The REDExtract-N-Amp PCR ReadyMix contains an inert dye that acts as a tracking dye and allows for convenient loading of PCR reaction onto agarose gels for analysis.

Sigma provides validated protocols to extract and amplify genomic DNA from animal and plant samples.

In a typical procedure, genomic DNA is extracted from a sample that has been incubated in preparation solution and extraction solution at room temperature. The sample is heated and then mixed with a third solution to neutralize inhibitory substances prior to PCR. A portion of the DNA extract is then added to a PCR reaction containing primers and the ReadyMix included in the kit. The remainder of the DNA can be refrigerated for convenient storage.

Typical Applications include:

  • Genotyping
  • Transgene detection
  • Knockout analysis
  • Sequencing

 

Product Speed Product Description Starting Material
Extract-N-Amp™ Tissue PCR Kit

Extract-N-Amp™ REDExtract-N-Amp Tissue PCR Kit
Less than 15 minutes from extraction to amplification
Rapid amplification of genomic DNA from
  • Mouse-tails
  • Hair
  • Animal Tissue
  • Buccal Swabs
Tail: 0.5 to 1.0 cm

Hair: 1 root

Tissue: 2 to 10 mg

Buccal: 10 µl
SYBR® Green Extract-N-Amp™ Tissue PCR Kit Less than 15 minutes from extraction to amplification
Rapid quantitative PCR of genomic DNA from
  • Mouse-tails
  • Hair
  • Animal Tissue
  • Buccal Swabs
Tail: 0.5 to 1.0 cm

Hair: 1 root

Tissue: 2 to 10 mg

Buccal: 10 µl
Extract-N-Amp™ Blood PCR Kit

REDExtract-N-Amp Blood PCR Kit

8 minutes from extraction to amplification Rapid amplification of genomic DNA from
  • Whole blood
Blood: 10 µl
Extract-N-Amp™ Plant PCR Kit

REDExtract-N-Amp Plant PCR Kit
Less than 15 minutes from extraction to amplification Rapid extraction and PCR amplification of genomic DNA from
  • Plant leaves
Plant Leaf Disk:
0.5 to 0.7 cm
SYBR® Green Extract-N-Amp™ Plant PCR Kit Less than 15 minutes from extraction to amplification Rapid extraction and PCR amplification of genomic DNA from
  • Plant leaves
Plant Leaf Disk:
0.5 to 0.7 cm
Extract-N-Amp™ Seed PCR Kit

REDExtract-N-Amp Seed PCR Kit

Less than 15 minutes from extraction to amplification Rapid amplification of genomic DNA from
  • Plant seeds
1 seed

 

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