Identification of Proteins in Lipofuscin Using Antibodies within the Human Protein Atlas

By: Evelina Sjöstedt1, Dijana Djureinovic1, Jan Mulder2 and Caroline Kampf1, Poster - The Human Potein Atlas
1Department of Immunology, Genetics and Pathology, Human Protein Atlas, Science for Life Laboratory, Uppsala University, Uppsala, Sweden
2Department of Neuroscience, Human Protein Atlas, Science for Life Laboratory, Karolinska Institute, Stockholm, Sweden

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Background

The Human Protein Atlas (HPA) is a research project that aims to visualize the cellular and subcellular location of every human protein by using monospecific polyclonal antibodies1. This high-throughput approach enables the discovery of new protein distribution profiles and can also provide deeper insights into already known patterns. Here we used HPA antibodies to identify proteins associated with the age pigment lipofuscin.

Lipofuscin is mainly present in post mitotic cells. It consists of lipids, proteins, carbohydrates and a small amount of metals. Despite that lipofuscin has been well-known for a long time the exact composition of the protein portion is uncertain2.

Collection of Proteins in Lipofuscin

Proteins within Lipofuscin

Conclusions

All methods; histochemical staining, confocal imaging and Western blot verified that the staining pattern observed by the antibodies was indeed lipofuscin.

The large collection of antibodies produced within the HPA project has proven to be a valuable tool to identify compartments of cellular complexes like lipofuscin.

A number of the previously unknown proteins within lipofuscin have been identified; GGH (HPA025226), MIP18 (HPA041736) and HDGL1 (HPA045679). Thereby a few pieces of the lipofuscin puzzle have been solved.

 

References

  1. A human protein atlas for normal and cancer tissues based on antibody proteomics. Mol Cell Proteomics. 2005 4 (12):1920-32
  2. Lipofuscin: Mechanisms of age-related accumulation and influence on cell functions. Free Radical Biology & Medicine, 33 (2002), pp. 611-619

 

The HPR project is funded by the Knut & Alice Wallenberg foundation. The atlas is part of the HUPO Human Antibody Initiative (HAI).

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