Custom Oligos for Commercial Use

We offer custom DNA Primers, qPCR Probes, and Next-Gen Sequencing Oligos for Life Science Research Tools, Molecular Diagnostics, and Laboratory Developed Tests. We can also perform component or complete kit manufacturing, including custom formulations, packaging, and private labeling. Learn about our manufacturing capabilities or request a consultation to determine which type of product best fits your needs.

 



Life Science Research Tools

ISO 9001:2008

 

Custom Primers and SYBR® Green Primers
Scales 0.025, 0.05, 0.2, 1.0, 10, and 15 μmol
Purifications Desalt, Cartridge, RP/IE-HPLC, and PAGE
Sequence Lengths 10 - 120 bases
Modifications Over 200 available, including dyes (fluorescent and non-fluorescent)
Quality Control 100% mass spectrometry
Format Supplied dry or in solution - tubes, plates (96 or 384-well; concentration normalization with fixed or variable volumes; customized plates and buffers; 1D or 2D barcoding; and, formatting options including plate copies), and mixed plates (forward and reverse primers)

 

Custom qPCR Probes, i.e. Dual-Labeled Probes
Amounts
1, 3, 5, and 10 OD
Purification HPLC
Sequence Lengths 15 - 40 bases
Modifications LNA® available
Dyes Comprehensive offering of reporters and quenchers
Quality Control 100% mass spectrometry
Format Supplied dry in amber tubes
Also Available
Molecular Beacons, LightCycler® Probes, and Scorpions® Probes
  • iScale Oligos™ milligram quantities of primers and 50/100 OD quantities of probes for high-throughput needs
  • Dedicated, single-point-of-contact project management from the start of the project through order fulfillment
  • Product development support for creating innovative approaches to novel oligonucleotide constructs or formulations
  • Process development support for working with unique methods or adopting your existing protocols to meet specifications
  • Component or complete kit manufacturing

Molecular Diagnostics

ISO 13485:2003

 

Custom Primers and SYBR® Green Primers
Scales 0.2, 1.0, 10, and 15 µmol
Purifications Desalt, Cartridge, and RP-HPLC
Sequence Lengths 10 - 70 bases (inquire for others)
Modifications 5’ Phosphate (inquire for others)
Quality Control 100% mass spectrometry
Format Supplied dry or in solution – tubes and plates (inquire for mixed plates)

 

Custom qPCR Probes, i.e. Dual-Labeled Probes
Amounts 1, 3, 5, and 10 OD
Purification HPLC
Sequence Lengths 15 - 40 bases
Dyes Reporters—6-FAM™, HEX™, JOE™, TAMRA™, ROX™, and Cyanine 5
Quenchers—TAMRA™ and Onyx Quencher™
Quality Control 100% mass spectrometry
Format Supplied dry in amber tubes
  • Up to 100 mg of primers for high-throughput needs
  • Dedicated, single-point-of-contact project management from the start of the project through order fulfillment

Onyx Quencher™ (OQ™)

  • Sigma’s proprietary dark quencher for probe-based qPCR
  • Available on the 3’ end with Custom Primers and Dual-Labeled Probes
  • Performance validated with several common dyes in the 5’ nuclease assay against a comparable dark quencher
  • May be used for any application in addition to qPCR—perfect for life science research tools, molecular diagnostics, and laboratory developed tests
  • Learn More

Next-Gen Sequencing Oligos

  • Custom universal and index (barcode) adapter oligos for major sequencing platforms
  • Suitable purity (percent full-length sequence) to maintain sequence read integrity
  • Low levels of cross-contamination to facilitate proper sequence assembly
  • Learn More

Quality Assurance

Service Description
Customer defined certificates of analysis Meets your incoming release specifications and streamlines your product acceptance process
Document control Retention of files, including detailed batch records for ISO 13485 manufacturing
Customer change notification Ensures transparency and risk mitigation
Vendor management Provides complete material traceability throughout the manufacturing process
Business agreements Establishment of confidential disclosure and supply agreements ensures confidentiality, product requirements, and continuity of supply are achieved
Corrective and preventive actions Elimination and prevention of nonconformances

Support Services

  • Functional testing of your product, regulatory compliance consulting, audits of any of our facilities, forecasting / capacity planning, account-specific performance indicators, business reviews, inventory management, and warehousing

Materials