Manganese (II) chloride solution for molecular biology

Product No. M1787

CAS RN 7773-01-5
Synonyms: manganous chloride, manganese dichloride

Product Description

This product is a 1.00 ± 0.01 M solution, prepared in 18 megohm water, and is 0.2 µm filtered. It is designated as Molecular Biology grade and is suitable for molecular biology applications. This product has been analyzed for the absence of DNases and proteases.

Manganese chloride occurs in nature as the mineral scacchite. It is used in industrial applications such as dyeing and disinfecting, the purification of natural gas, and as a drying agent for linseed oil.1

In biochemistry, manganese is a cofactor in restriction enzymes such as BamH I and EcoR V.2,3 MnCl2 is used as a source of manganese ion in biological research, such as in studies of manganese uptake in rat liver and of inhibition of Mycoplasma strain growth.4,5 The alteration in gene expression of glutamate/aspartate transporter, taurine transporter, and metallothionein I in astrocytes that have been exposed to MnCl2 has been studied.6

Manganese chloride has been substituted for magnesium chloride to investigate alterations in activity of the Herpes simplex virus type 1 DNA polymerase.7 MnCl2 has been shown to inhibit choline transport at the blood brain barrier in rats.8

Storage

Room Temperature

Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices. 

Materials

     

 References

  1. The Merck Index, 12th ed., Entry# 5768.
  2. Viadiu, H., and Aggarwal, A. K., The role of metals in catalysis by the restriction endonuclease BamHI. Nat. Struct. Biol., 5(10), 910-916 (1998).
  3. Stanford, N. P., et al., DNA cleavage by the EcoRV restriction endonuclease: pH dependence and proton transfers in catalysis. J. Mol. Biol., 288(1), 105-116 (1999).
  4. Colet, J. M., et al., Dynamic evaluation of the hepatic uptake and clearance of manganesebased MRI contrast agents: a 31P NMR study on the isolated and perfused rat liver. J. Magn. Reson. Imaging, 8(3), 663-669 (1998).
  5. Watanabe, T., Effects of manganese on growth of Mycoplasma salivarium and Mycoplasma orale. J. Clin. Microbiol., 32(5), 1343-1345 (1994).
  6. Erikson, K. M., et al., Glutamate/aspartate transporter (GLAST), taurine transporter and metallothionein mRNA levels are differentially altered in astrocytes exposed to manganese chloride, manganese phosphate or manganese sulfate. Neurotoxicology, 23(3), 281-288 (2002).
  7. Villani, G., et al., Effect of manganese on in vitro replication of damaged DNA catalyzed by the herpes simplex virus type-1 DNA polymerase. Nucleic Acids Res. 30(15), 3323-3332 (2002).
  8. Lockman, P. R., et al., Inhibition of the rat bloodbrain barrier choline transporter by manganese chloride. J. Neurochem., 79(3), 588-594 (2001).

 

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