MISSION® siRNA Universal Negative Controls

MISSION® siRNA Universal Negative Controls are an essential component to any siRNA experiment. Using a negative siRNA control allows the researcher to create a baseline for mRNA knockdown efficiency. MISSION siRNA Universal Negative Control sequences have been carefully designed to have no homology to known gene sequences, and have been tested in human, rat, and mouse cells

Features and Benefits

  • MISSION® siRNA Universal Negative Controls are available unlabeled and fluorescently-labeled (Cyanine 3, Cyanine 5, and 6-FAM®).
  • Easily evaluate transfection efficiency and optimize transfection conditions with the fluorescent-labeled siRNA negative controls.
  • Ready to use and available in convenient quantities: 1 nmol, 5x1nmol bundle, and 10 nmol
  • Custom modification and quantities available. Learn more

Easily Visualize siRNA Transfection with the MISSION siRNA Fluorescent Universal Negative Controls

Transfection efficiency and experimental conditions can be assessed quickly and effortlessly by transfecting the MISSION siRNA Fluorescent Universal Negative Controls in the desired cell line. HeLa cells were seeded in 24-well plates 24 hours prior to transfection at a density of 40,000 cells per well. Cells were then transfected using the MISSION siRNA Transfection Reagent (S1452) and 10 nM of the MISSION siRNA Fluorescent Universal Negative Controls. Fluorescence was assessed 24 hours post-transfection, and images captured at 40x magnification. Figures 1, 2, and 3 show examples of expected results. Figure 4 shows transfection control cells.

Fluorescence and differential interference contrast (DIC) microscopy of MISSION siRNA Fluorescent Universal Negative Control #1, Cyanine 3

Figure 1. Fluorescence and differential interference contrast (DIC) microscopy of MISSION siRNA Fluorescent Universal Negative Control #1, Cyanine 3 (Cat. No. SIC003). Arrows indicate a cell where fluorescence is evident.  Cyanine 3 excites at 550 nm and emits at 570 nm. The RFP filter used excites at 555 nm±25 and detects at 605 nm±52.

Fluorescence and DIC microscopy of MISSION siRNA Fluorescent Universal Negative Control #1, Cyanine 5

Figure 2. Fluorescence and DIC microscopy of MISSION siRNA Fluorescent Universal Negative Control #1, Cyanine 5 (Cat. No. SIC005). Arrows indicate a cell where fluorescence is evident. Cyanine 5 excites at 650 nm and emits at 670 nm. The Cyanine 5 filter used excites at 320 nm±60 and detects at 700 nm±75.

Fluorescence and DIC microscopy of MISSION siRNA Fluorescent Universal Negative Control #1, 6-FAM

Figure 3. Fluorescence and DIC microscopy of MISSION siRNA Fluorescent Universal Negative Control #1, 6-FAM (Cat. No. SIC007). Arrows indicate a cell where fluorescence is evident.  6-FAM excites at 495 nm and emits at 516 nm. The GFP filter used excites at 490 nm±20 and detects at 525 nm±36.

Microscopy of HeLa cells with transfection control (transfection reagent only)

Figure 4. Microscopy of HeLa cells with transfection control (transfection reagent only). A) DIC image, and fluorescence microscopy using B) Cyanine 5 filter, C) GFP filter, and D) RFP filter. Some auto fluorescence is observed, however, it is very dim compared to MISSION siRNA Fluorescent Universal Negative Control.

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