Nucleic Acid Modifying Enzyme Selection Chart (Ultra Pure)

Find more protocols and selection guides in the Molecular Biology Guide.

DNA Polymerases (ultra pure)

DNA Polymerases synthesize DNA from nucleotides.  DNA polymerase is required for DNA replication, but also essential for many other activities in the cell, including genetic recombination, DNA repair, and reverse transcription.  In molecular biology labs, DNA polymerases are used for applications such as cloning, PCR, and DNA sequencing.

Each DNA polymerase has special characteristics, making it useful for specific applications.  Knowing these characteristics enables you to pick the right ultra pure DNA Polymerase for your application.

DNA Polymerase Selection Chart

Product Number Product Description Notable Characteristics Application Suitability
KEM0026 Klenow (3’ → 5’ exo) • Deficient in both proofreading and nick translation nuclease activities
• Moderate strand displacement activity
• A-tailing for NGS
• Strand displacement amplification
• DNA labeling
KEM0027 Klenow (3’ → 5’ exo)
KEM0028 phi29 DNA Polymerase (High concentration) • Highly processive
• Proofreading activity (3’ → 5’)
• Powerful strand displacement activity
• Whole Genome Amplification
KEM0029 phi29 DNA Polymerase (Low concentration)
KEM0030 DNA Polymerase I • Exhibits 3’ → 5’ synthesis activity
• Exonuclease activities (both 3’ → 5’ and 5’ → 3’)
• Second strand cDNA synthesis
• Nick-translation
• DNA labeling
KEM0031 Klenow Fragment
• Exhibits synthesis and proofreading (3’ → 5’) nuclease activities
• Moderate strand displacement activity
• DNA blunting by fill-in of 5’ overhang
• Second strand cDNA synthesis
• Sequencing
• Site-directed mutagenesis

KEM0032 Terminal deoxynucleotidyl Transferase (TdT) • Template-independent
• Catalyzes addition of deoxynucleotides to 3’OH of ss- or ds-DNA
• Homopolymeric tailing to the 3’OH
• TUNEL assay
• 5’ RACE
• Labeling of 3’ end
KEM0033 T4 DNA Polymerase • Catalyzes extension of a primed DNA template (5’ → 3’ direction)
• Powerful 3’ → 5’ exonuclease activity
• Lacks inherent 5’ → 3’ exonuclease or strand displacement activity
• DNA end-repair by fill-in of 5’ overhang
• Removal of 3’ overhang
KEM0034 Mako DNA Polymerase
(3’ → 5’ exo)
• Catalyzes extension of a primed DNA template (5’ → 3’ direction) • Protocols requiring no 3’ → 5’ or 5’ → 3’ exonuclease activity
• Applications requiring no strand displacement activity
KEM0035 Manta 1.0 DNA Polymerase
(High concentration)
• Thermophilic enzyme
• Deficient in both proofreading and nick translation nuclease activities
• Applications requiring an enzyme with thermo-stable, strong strand replacement activity
• Applications requiring a Bst I large fragment equivalent
• SDA assy
• LAMP assay
• NEAR assay
KEM0036 Manta 1.0 DNA Polymerase
(Low concentration)

DNA Ligases (ultra pure)

DNA Ligases form phosphodiester bonds between DNA strands, joining them together.  DNA Ligase is responsible for repairing breaks in single and double stranded DNA.  In molecular biology labs, DNA Ligase is commonly used for molecular cloning applications.

Each DNA Ligase has special characteristics, making it useful for specific applications.  Knowing these characteristics enables you to pick the right ultra pure DNA Ligase for your application.

DNA Ligase Selection Chart

Product Description T4 DNA Ligase
T4 DNA Ligase (Rapid)
T3 DNA Ligase
T7 DNA Ligase
E. coli DNA Ligase
Products No. KEM0020 KEM0019 KEM0017 KEM0018 KEM0025
Units
150,000 240,000 900,000 900,000 2,500
Concentration 120,000 U/mL 600,000 U/mL 3,000,000 U/mL 3,000,000 U/mL 40,000 U/mL
Cofactor Required ATP ATP ATP ATP NAD
Recommended for Cloning?      
Recommended for cohesive ends
Recommended for blunt ends
(less efficient)

(less efficient)

(less efficient)
Recommended for nicks in dsDNA
Recommended for joining of
RNA & DNA hybrids
     
Application Suitability • Restriction cloning
• TA cloning
• Adapter ligation
• NGS library construction
• Restriction cloning
• TA cloning
• Adapter ligation
• Applications requiring good activity under high ionic strength (1M NaCl) • TALE
• Applications requiring high cohesive end ligation efficiency
• cDNA cloning by replacement synthesis

Nucleic Acid Modifying Enzymes (ultra pure)

Many different enzymes exist that are able to modify DNA, RNA, and RNA:DNA hybrids.  In molecular biology labs, these modifying enzymes are commonly used for molecular cloning and sequencing applications.

Each enzyme has special characteristics, making it useful for specific applications.  Knowing these characteristics enables you to pick the right ultra pure Nucleic Acid Modifying Enzyme for your application.

Nucleic Acid Modifying Enzyme Selection Chart

Product No. Product Description Notable Characteristics Application Suitability
KEM0006 T4 Polynucleotide Kinase
• Catalyzes the transfer and exchange of ATP to 5’OH of ss- or ds-DNA and RNA
• End-labeling DNA or RNA for probes
• End-labeling DNA or RNA for sequencing
• Addition of 5’ phosphates to oligonucleotides for subsequent ligation
• Removal of 3’ phosphoryl groups
KEM0001 Uracil DNA Glycosylase (UDG) • Catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar (leaving an abasic site) • Removal of uracil from DNA
• Control of carry-over contamination in PCR
KEM0002 Thermolabile Uracil DNA Glycosylase (UDG) • Catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar (leaving an abasic site)
• Completely inactivated in 1x reaction buffer at 50°C for 10 minutes.
KEM0004 Exonuclease III • Digests one strand of the dsDNA at a time
• Digests the RNA strand of RNA:DNA hybrids
• Removal of nucleotide(s) from duplex DNA in the 3’ → 5’ direction
KEM0005 Lambda Exonuclease • Highly processive 5’ → 3’ double-stranded exonuclease
• Degrades one strand of the duplex
• Highly processive 5’ → 3’ exonuclease
• Applications requiring activity on blunt and 5’ recessed ends
KEM0009 End-Repair Mix
(High Concentration)
• Mix of T4 DNA Polymerase and T4 Polynucleotide Kinase
• Converts to blunt-ended DNA
• Converting DNA containing damaged or incompatible 5’ and/or 3’ protruding ends to 5’ phosphoryleted, blunt-ended DNA
KEM0010 End-Repair Mix
(Low Concentration)
KEM0011 10x Uracil Cleavage System • Generates a single nucleotide gap at uracil residues • Excising uracil residues from DNA
KEM0014 Poly(A) Polymerase • Catalyzes the addition of AMP (from ATP) to the RNA 3’OH • Labeling of RNA with ATP or cordycepin
• Poly(A) tailing of RNA for cloning or affinity purification
• Translation of RNA transferred into eukaryotic cells
KEM0015 RNase H • Degrades the RNA strand of RNA:DNA hybrids • Removal of mRNA during second-strand cDNA synthesis
• Cleavage of RNA from RNA:DNA hybrids

RNA Ligases (ultra pure)

RNA Ligases form phosphodiester bonds between RNA strands, joining them together.  In molecular biology labs, RNA Ligase is commonly used for mutagenesis of RNA, and end-labeling applications.

Each RNA Ligase has special characteristics, making it useful for specific applications.  Knowing these characteristics enables you to pick the right ultra pure RNA Ligase for your application.

RNA Ligase Selection Chart

Product Description T4 RNA Ligase I T4 RNA Ligase 2 T4 RNA Ligase 2 (truncated)
Product No. KEM0021 KEM0024 KEM0023
Units
10,000 4,500 500
Concentration 20,000 U/mL 30,000 U/mL 5,000 U/mL
Cofactor Required ATP
ATP
None
Recommended for nicks in dsDNA?    
Recommended for joining of RNA:DNA hybrids?    
Recommended for labeling of RNA 3’ termini?  
Recommended for joining ssDNA?    
Recommended for joining ssRNA?    
Related Links