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Plasma profiling of ageing and disease on antibodies

By: Jochen M. Schwenk, Biobank Profiling Group, Peter Nilsson and Mathias Uhlén, Poster - The Human Potein Atlas
Science for Life Laboratory Stockholm, KTH -­- Royal InsJtute of Technology, Box 1031, SE-­-171 21 Solna, Sweden

Download the poster (2.2 Mb PDF)

Affinity proteomic profiling of plasma

High-throughput methods for screening samples stored in biobanks are of growing interest, since they would allow to discover biomarkers of ageing and disease for diagnostics and patient care at a larger scale. With more than 15,000 well-characterised affinity reagents in the Human Protein Atlas (www.proteinatlas.org), a systematic exploration of protein profiles from serum and plasma can be achieved by single-binder affinity arrays. Assays have been developed that profile up to 384 proteins in each of the 384 labeled, non-fractionated samples in parallel on magnetic, color-coded beads.

References - Uhlén M, et al. (2010) Nat Biotech. 28(12): 1248-50; Schwenk JM, et. al. (2010) Mol Cell Prot. Nov;9(11):2497-507


Strategy for profiling of plasma using antibodies

Antibodies are being generated based on bioinformatics target selection and selected based on specificity and concentration. Single-binder bead arrays are then created with 384 antibodies each. Studies are then designed by the randomization of to apply bead arrays for profiling discovery cohorts. Interesting antibodies are then qualified for further verification in new cohorts. The overall aim is to build sandwich assays for qualification via epitope mapped binders.

Strategy for profiling of plasma using antibodies


Exploration of plasma protein profiles

The affinity--based strategy has been applied to explore profiles males and female aged 5-85 (n=384) as well as to diseases. For the latter, more than 20 different disease entities (n=570) were profiled in parallel and to propose antibodies for single disease verification.


Identification of protein profiles associated with age

Linear regression (left); Loess Clustering (mid); Signature score (right)

Identification of protein profiles associated with age


Identification of protein profiles associated with disease

The boxplots represent the differential plasma protein profiles generated by four different antibodies for matched comparisons in Alzheimer's Disease (alz) and Mild Cognitive Impairment (mci).

Identification of protein profiles associated with disease

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