Attention:

Certain features of Sigma-Aldrich.com will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

Protease Inhibitor Cocktails References

The Sigma-Aldrich portfolio of protease inhibitor cocktails shows an excellent track record in the peer-reviewed scientific literature, as witnessed by the many publications that cite our protease inhibitor cocktails.  This page lists a sampling of those research papers.  Other citations are available under the specific product pages for each of our protease inhibitor cocktails, under the “Peer-Reviewed Papers” tab.

A Rudich, S Vanounou, K Riesenberg, M Porat, A Tirosh, I Harman-Boehm, A S Greenberg, F Schlaeffer, N Bashan
Diabetes 2001-06-01
HIV protease inhibitors (HPIs) are potent antiretroviral agents clinically used in the management of HIV infection. Recently, HPI therapy has been linked to the development of a metabolic syndrome in which adipocyte insulin resistance appears to play a major role. In this study, we assessed the effect of nelfinavir on glucose up...Read More
Frank R Wettey, Steve F C Hawkins, Abigail Stewart, J Paul Luzio, Jonathan C Howard, Antony P Jackson
Science 2002-08-30
We exploited the high rate of homologous recombination shown by the chicken B cell line DT40 to inactivate the endogenous alleles for clathrin heavy chain and replace them with human clathrin complementary DNA under the control of a tetracycline-regulatable promoter. Clathrin repression perturbed the activities of Akt-mediated a...Read More
Francisca Rodriguez, Brian D Lamon, Weiying Gong, Rowena Kemp, Alberto Nasjletti
Hypertension 2004-02-01
We tested the hypothesis that the status of NO synthesis influences the renal heme-heme oxygenase system. Studies were conducted in untreated rats and rats treated with the NO synthesis inhibitor N(G)-nitro-L-arginine methyl ester for 2 days. Treated and untreated rats were contrasted in terms of renal expression of heme oxygena...Read More
Sang Ki Park, Minh Dang Nguyen, André Fischer, Margaret Po-Shan Luke, El Bachir Affar, Paul Brian Dieffenbach, Huang-Chun Tseng, Yang Shi, Li-Huei Tsai
Cell 2005-07-29
Prostate apoptosis response 4 (Par-4) is a leucine zipper containing protein that plays a role in apoptosis. Although Par-4 is expressed in neurons, its physiological role in the nervous system is unknown. Here we identify Par-4 as a regulatory component in dopamine signaling. Par-4 directly interacts with the dopamine D2 recept...Read More
Bin Zeng, Xiaomin Cai, Guan Zhu
Microbiology 2006-08-01
In this paper, the identification and functional analysis of a fatty acyl-CoA-binding protein (ACBP) gene from the opportunistic protist Cryptosporidium parvum are described. The CpACBP1 gene encodes a protein of 268 aa that is three times larger than typical ACBPs (i.e. approximately 90 aa) of humans and animals. Sequence analy...Read More
C C Villar, H Kashleva, C J Nobile, A P Mitchell, A Dongari-Bagtzoglou
Infection and Immunity 2007-05-01
The ability of Candida albicans to invade mucosal tissues is a major virulence determinant of this organism; however, the mechanism of invasion is not understood in detail. Proteolytic breakdown of E-cadherin, the major protein in epithelial cell junctions, has been proposed as a mechanism of invasion of certain bacteria in the ...Read More
Majid Ebrahimi, Linda Boldrup, Philip J Coates, Ylva-Britt Wahlin, Jean-Christophe Bourdon, Karin Nylander
Oral Oncology 2008-02-01
Oral lichen planus (OLP) is a chronic inflammatory disease of unknown origin, showing little spontaneous regression. WHO classifies OLP as a premalignant condition, however, the underlying mechanisms initiating development of cancer in OLP lesions are not understood. The p53 tumour suppressor plays an important role in many tumo...Read More
L Vojtová, T Zima, V Tesar, M Kazderová
Folia Biologica (Prague) 2007-01-01
Heavy proteinuria may be caused by either increased glomerulal basement membrane permeability or membrane or podocyte structural damage, and also by impairment of secretion-reabsorption tubular processes. The precise composition of modified or degraded urine proteins in proteinuria is not known. However, a possible toxic effect ...Read More
R Rashmi, S G Pillai, S Vijayalingam, J Ryerse, G Chinnadurai
Oncogene 2008-02-28
The BH3-only protein BIK normally induces apoptotic cell death. Here, we have investigated the role of BCL-2 in BIK-induced cell death using Bcl-2+/+ and Bcl-2-/- mouse embryo fibroblasts. Ectopic expression of BIK in Bcl-2-/- cells resulted in enhanced cell death compared to Bcl-2+/+ cells. In these cells, while caspase-8 was a...Read More
Kumaran Sundaram, Santhosh K Mani, Kazuyuki Kitatani, Kongming Wu, Richard G Pestell, Sakamuri V Reddy
Journal of Cellular Biochemistry 2008-04-15
Receptor activator of NF-kappaB ligand (RANKL) is a critical osteoclastogenic factor that is expressed on bone marrow stromal/preosteoblast cells. Most bone resorption stimuli induce osteoclast formation by modulating RANKL expression in these cells. However, little is known about the mechanisms regulating RANKL gene expression....Read More
Lan Lin, Elizabeta Nemeth, Julia B Goodnough, Dharma R Thapa, Victoria Gabayan, Tomas Ganz
Blood Cells, Molecules, and Diseases 2008-01-01
As the principal iron-regulatory hormone, hepcidin plays an important role in systemic iron homeostasis. The regulation of hepcidin expression by iron loading appears to be unexpectedly complex and has attracted much interest. The GPI-linked membrane protein hemojuvelin (GPI-hemojuvelin) is an essential upstream regulator of hep...Read More
Artem G Ayuyan, Fredric S Cohen
Biophysical Journal 2008-04-01
Biological rafts were identified and isolated at 37 degrees C and neutral pH. The strategy for isolating rafts utilized membrane tension to generate large domains. For lipid compositions that led only to microscropically unresolvable rafts in lipid bilayers, membrane tension led to the appearance of large, observable rafts. The ...Read More
Richard Milner, Stephanie Hung, Xiaoyun Wang, Maria Spatz, Gregory J del Zoppo
Journal of Cerebral Blood Flow and Metabolism 2008-04-01
During focal cerebral ischemia, the detachment of astrocytes from the microvascular basal lamina is not completely explained by known integrin receptor expression changes. Here, the impact of experimental ischemia (oxygen-glucose deprivation (OGD)) on dystroglycan expression by murine endothelial cells and astrocytes grown on va...Read More
Michele F Hannah, Vladimir B Bajic, Sabra L Klein
Brain, Behavior, and Immunity 2008-05-01
Among rodents that carry hantaviruses, more males are infected than females. Male rats also have elevated copies of Seoul virus RNA and reduced transcription of immune-related genes in the lungs than females. To further characterize sex differences in antiviral defenses and whether these differences are mediated by gonadal hormo...Read More
Christine Linard, Olivier Grémy, Marc Benderitter
Journal of Pharmacology and Experimental Therapeutics 2008-03-01
Radiation-induced intestinal injuries, including inflammation and immune response, remain a limiting factor in the effectiveness of pelvic radiotherapy and in the patient's quality of life during and after treatment. Peroxisome proliferation-activated receptor (PPAR) agonists are now emerging as therapeutic drugs for various inf...Read More
Samar M Hammad, Heather G Crellin, Bill X Wu, Jessica Melton, Viviana Anelli, Lina M Obeid
Prostaglandins and Other Lipid Mediators 2008-03-01
Sphingosine kinase 1 (SK1) and its product sphingosine-1-phosphate (S1P) have been implicated in the regulation of many cellular processes including growth regulation, protection from apoptosis, stimulation of angiogenesis, and most recently as mediators of the TNF-alpha inflammatory response. In this study we set out to examine...Read More
Anca D Petrescu, Huan Huang, Heather A Hostetler, Friedhelm Schroeder, Ann B Kier
Protein Expression and Purification 2008-04-01
Acyl coenzyme A binding protein (ACBP) has been proposed to transport fatty acyl CoAs intracellularly, facilitating their metabolism. In this study, a new mouse recombinant ACBP was produced by insertion of a histidine (his) tag at the C-terminus to allow efficient purification by Ni-affinity chromatography. The his-tag was inse...Read More
Julie M Bailis, Douglas D Luche, Tony Hunter, Susan L Forsburg
Molecular and Cellular Biology 2008-03-01
The minichromosome maintenance (MCM) complex plays essential, conserved roles throughout DNA synthesis: first, as a component of the prereplication complex at origins and, then, as a helicase associated with replication forks. Here we use fission yeast (Schizosaccharomyces pombe) as a model to demonstrate a role for the MCM comp...Read More
Natalie A Prow, David N Irani
Journal of Neurochemistry 2008-05-01
Astrocytes remove glutamate from the synaptic cleft via specific transporters, and impaired glutamate reuptake may promote excitotoxic neuronal injury. In a model of viral encephalomyelitis caused by neuroadapted Sindbis virus (NSV), mice develop acute paralysis and spinal motor neuron degeneration inhibited by the AMPA receptor...Read More
Yong Pan, Edward J Weinman, Jia Le Dai
Breast Cancer Research (Online Edition) 2008-01-01
The gene encoding Na+/H+ exchanger regulatory factor 1 (NHERF1) is a putative tumor suppressor gene that harbors frequent loss of heterozygosity (LOH) and intragenic mutations in breast carcinoma. The exact biologic activity of NHERF1 in mammary glands, however, remains unclear. It was recently proposed that NHERF1 forms a terna...Read More
John Arne Dahl, Philippe Collas
Nucleic Acids Research 2008-02-01
Chromatin immunoprecipitation (ChIP) is a powerful technique for studying protein-DNA interactions. Drawbacks of current ChIP assays however are a requirement for large cell numbers, which limits applicability of ChIP to rare cell samples, and/or lengthy procedures with limited applications. There are to date no protocols for fa...Read More
Asha Shahed, Kelly A Young
Molecular Reproduction and Development 2008-09-01
Siberian hamsters adapt to seasonal changes by reducing their reproductive function during short days (SD). SD exposure reduces uterine mass and reproductive capacity, but underlying cellular mechanisms remain unknown. Because matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) are important in uterine developme...Read More
Alicia Plumer, Hongyi Duan, Sripriya Subramaniam, F Lee Lucas, Susan Miesfeldt, Ah-Kau Ng, Lucy Liaw
BMC Cancer 2008-01-01
Osteopontin (OPN) is associated with human cancers, and circulating blood OPN may have diagnostic or prognostic value in clinical oncology. To evaluate OPN as a cancer biomarker, we generated and characterized five novel mouse monoclonal antibodies against the human full-length OPN (fl-OPN). Epitopes recognized by four antibodie...Read More
Inés Pineda Torra, Naima Ismaili, Jonathan E Feig, Chong-Feng Xu, Claudio Cavasotto, Raluca Pancratov, Inez Rogatsky, Thomas A Neubert, Edward A Fisher, Michael J Garabedian
Molecular and Cellular Biology 2008-04-01
Dysregulation of liver X receptor alpha (LXRalpha) activity has been linked to cardiovascular and metabolic diseases. Here, we show that LXRalpha target gene selectivity is achieved by modulation of LXRalpha phosphorylation. Under basal conditions, LXRalpha is phosphorylated at S198; phosphorylation is enhanced by LXR ligands an...Read More
Stefan Gröschel, Kisha D Piggott, Augusto Vaglio, Wei Ma-Krupa, Karnail Singh, Jörg J Goronzy, Cornelia M Weyand
Journal of Molecular Medicine (Berlin, Germany) 2008-04-01
Dendritic cells (DCs) shape T-cell response patterns and determine early, intermediate, and late outcomes of immune recognition events. They either facilitate immunostimulation or induce tolerance, possibly determined by initial DC activation signals, such as binding Toll-like receptor (TLR) ligands. Here, we report that DC stim...Read More
H Bond, M R Dilworth, B Baker, E Cowley, A Requena Jimenez, R D H Boyd, S M Husain, B S Ward, C P Sibley, J D Glazier
Journal of Physiology 2008-04-01
The role of parathyroid hormone-related protein (PTHrP) in fetal calcium homeostasis and placental calcium transport was examined in mice homozygous for the deletion of the PTHrP gene (PTHrP-/- null; NL) compared to PTHrP+/+ (wild-type; WT) and PTHrP+/- (heterozygous; HZ) littermates. Fetal blood ionized calcium was significantl...Read More
Dominic Cosgrove, Daniel T Meehan, Duane Delimont, Ambra Pozzi, Xiwu Chen, Kathyrn D Rodgers, Richard M Tempero, Marisa Zallocchi, Velidi H Rao
The American Journal of Pathology 2008-03-01
Previous work has shown that integrin alpha1-null Alport mice exhibit attenuated glomerular disease with decreased matrix accumulation and live much longer than strain-matched Alport mice. However, the mechanism underlying this observation is unknown. Here we show that glomerular gelatinase expression, specifically matrix metall...Read More
Guowu Hu, Moshe Hacham, Scott R Waterman, John Panepinto, Soowan Shin, Xiaoguang Liu, Jack Gibbons, Tibor Valyi-Nagy, Keisuke Obara, H Ari Jaffe, Yoshinori Ohsumi, Peter R Williamson
Journal of Clinical Investigation 2008-03-01
Autophagy is a process by which cells recycle cytoplasm and defective organelles during stress situations such as nutrient starvation. It can also be used by host cells as an immune defense mechanism to eliminate infectious pathogens. Here we describe the use of autophagy as a survival mechanism and virulence-associated trait by...Read More
Hernan Roca, Renny T Franceschi
Nucleic Acids Research 2008-03-01
Chromatin immunoprecipitation (ChIP) is a widely used technique for quantifying protein-DNA interactions in living cells. This method commonly uses fixed (crosslinked) chromatin that is fragmented by sonication (X-ChIP). We developed a simple new ChIP procedure for the immunoprecipitation of sonicated chromatin isolated from ost...Read More
Gang Liu, Young-Jun Park, Edward Abraham
FASEB Journal 2008-07-01
Interleukin-1 receptor-associated kinase (IRAK) -1 plays an essential role in Toll-like receptor/interleukin-1 receptor (TLR/IL-1R) -associated NF-kappaB activation through its involvement in IKK activation, which then leads to subsequent IkappaB degradation and NF-kappaB nuclear translocation. In the present studies, we demonst...Read More
Perrine Barraud, Xiaoling He, Maeve A Caldwell, Robin Jm Franklin
BMC Neuroscience 2008-01-01
The olfactory epithelium is a neurogenic tissue comprising a population of olfactory receptor neurons that are renewed throughout adulthood by a population of stem and progenitor cells. Because of their relative accessibility compared to intra-cranially located neural stem/progenitor cells, olfactory epithelium stem and progenit...Read More
Edgar R Wood, Lisa M Shewchuk, Byron Ellis, Perry Brignola, Ronald L Brashear, Thomas R Caferro, Scott H Dickerson, Hamilton D Dickson, Kelly H Donaldson, Michael Gaul, Robert J Griffin, Anne M Hassell, Barry Keith, Robert Mullin, Kimberly G Petrov, Michael J Reno, David W Rusnak, Sarva M Tadepalli, John C Ulrich, Craig D Wagner, Dana E Vanderwall, Alex G Waterson, Jon D Williams, Wendy L White, David E Uehling
PNAS 2008-02-26
Analysis of the x-ray crystal structure of mono-substituted acetylenic thienopyrimidine 6 complexed with the ErbB family enzyme ErbB-4 revealed a covalent bond between the terminal carbon of the acetylene moiety and the sulfhydryl group of Cys-803 at the solvent interface. The identification of this covalent adduct suggested tha...Read More
Teresa Golden, Ileana V Aragon, Beth Rutland, J Allan Tucker, Lalita A Shevde, Rajeev S Samant, Guofei Zhou, Lauren Amable, Danalea Skarra, Richard E Honkanen
Biochimica et Biophysica Acta 2008-04-01
Ser/Thr protein phosphatase 5 (PP5) regulates several signaling-cascades that suppress growth and/or facilitate apoptosis in response to genomic stress. The expression of PP5 is responsive to hypoxia inducible factor-1 (HIF-1) and estrogen, which have both been linked to the progression of human breast cancer. Still, it is not c...Read More
Chikako Sakaguchi, Takashi Morishita, Hideo Shinagawa, Takashi Hishida
BMC Molecular Biology 2008-01-01
DNA double-strand breaks (DSBs) are induced by exogenous insults such as ionizing radiation and chemical exposure, and they can also arise as a consequence of stalled or collapsed DNA replication forks. Failure to repair DSBs can lead to genomic instability or cell death and cancer in higher eukaryotes. The Schizosaccharomyces p...Read More
Shunichi Takahashi, Spencer Whitney, Shigeru Itoh, Tadashi Maruyama, Murray Badger
PNAS 2008-03-18
Coral bleaching, caused by heat stress, is accompanied by the light-induced loss of photosynthetic pigments in in situ symbiotic dinoflagellate algae (Symbiodinium spp.). However, the molecular mechanisms responsible for pigment loss are poorly understood. Here, we show that moderate heat stress causes photobleaching through inh...Read More
Irina Maluykova, Oksana Gutsal, Marina Laiko, Anne Kane, Mark Donowitz, Olga Kovbasnjuk
Biochimica et Biophysica Acta 2008-06-01
Shiga toxins (Stx), released into the intestinal lumen by enterohemorrhagic Escherichia coli (EHEC), are major virulence factors responsible for gastrointestinal and systemic illnesses. These pathologies are believed to be due to the action of the toxins on endothelial cells, which express the Stx receptor, the glycosphingolipid...Read More
P Geisen, L J Peterson, D Martiniuk, Ahbineet Uppal, Y Saito, M Elizabeth Hartnett
Molecular Vision 2008-01-01
To study the effects of a neutralizing antibody to vascular endothelial growth factor (VEGF), given as an intravitreous injection, on intravitreous neovascularization (IVNV) and ongoing vascular development of avascular retina in a rat model relevant to human retinopathy of prematurity. Newborn Sprague-Dawley rats were exposed t...Read More
Nikita K Malavia, Justin D Mih, Christopher B Raub, Bao T Dinh, Steven C George
Respiratory Research 2008-01-01
Inflammatory cytokines (e.g. IL-13) and mechanical perturbations (e.g. scrape injury) to the epithelium release profibrotic factors such as TGF-beta2, which may, in turn, stimulate subepithelial fibrosis in asthma. We hypothesized that prolonged IL-13 exposure creates a plastic epithelial phenotype that is profibrotic through co...Read More
Jing Yang, Chaitanya Jain, Kurt Schesser
Journal of Bacteriology 2008-05-01
Yersinia spp. use a type 3 secretion system (T3SS) to directly inject six proteins into macrophages, and any impairment of this process results in a profound reduction in virulence. We previously showed that the exoribonuclease polynucleotide phosphorylase (PNPase) was required for optimal T3SS functioning in Yersinia pseudotube...Read More
Tze-Chen Hsieh, Zhirong Wang, Haiteng Deng, Joseph M Wu
Anticancer Research 2008-01-01
Resveratrol shows chemopreventive and other biological affects in in vitro and some animal studies. The bioactivities of resveratrol may be attributed to qualitative and quantitative differences in its cell-type-specific interaction and binding with its cellular targets, denoted as resveratrol targeting proteins (RTPs). To isola...Read More
Soshana Svendsen, Chad Zimprich, Mark G McDougall, Dieter H Klaubert, Georgyi V Los
BMC Cell Biology 2008-01-01
The ability to specifically label proteins within living cells can provide information about their dynamics and function. To study a membrane protein, we fused a multi-functional reporter protein, HaloTag, to the extracellular domain of a truncated integrin. Using the HaloTag technology, we could study the localization, traffick...Read More
Jing Huang, Pawel M Kaminski, John G Edwards, Albert Yeh, Michael S Wolin, William H Frishman, Michael H Gewitz, Rajamma Mathew
American Journal of Physiology - Lung Cellular and Molecular Physiology 2008-06-01
Monocrotaline (MCT)-induced pulmonary artery hypertension (PAH) in rats is preceded by an inflammatory response, progressive endothelial cell membrane disruption, reduction in the expression of caveolin-1, and reciprocal activation of STAT3 (PY-STAT3). Superoxide and NF-kappaB have been implicated in PAH. To evaluate the role of...Read More
Yoshiaki Hasegawa, Gena D Tribble, Henry V Baker, Jeffrey J Mans, Martin Handfield, Richard J Lamont
Infection and Immunity 2008-06-01
The SerB protein of Porphyromonas gingivalis is a HAD family serine phosphatase that plays a critical role in entry and survival of the organism in gingival epithelial cells. SerB is secreted by P. gingivalis upon contact with epithelial cells. Here it is shown by microarray analysis that SerB impacts the transcriptional profile...Read More
Hema M Vasudeva-Rao, Kathleen A McDonough
Infection and Immunity 2008-06-01
Little is known about how Mycobacterium tuberculosis regulates gene expression in response to its host environment, despite its importance as a pathogen. We previously characterized 10 acr-coregulated genes (ACGs), all of which belong to the DevR (DosR) "dormancy" regulon, and identified one to three copies of a conserved 18-bp ...Read More
Susumu Morigasaki, Koichi Shimada, Aminah Ikner, Mitsuaki Yanagida, Kazuhiro Shiozaki
Molecular Cell 2008-04-11
Phosphorelay signaling of environmental stimuli by two-component systems is prevailing in bacteria and also utilized by fungi and plants. In the fission yeast Schizosaccharomyces pombe, peroxide stress signals are transmitted from the Mak2/3 sensor kinases to the Mpr1 histidine-containing phosphotransfer (HPt) protein and finall...Read More
Akira Nukazuka, Hajime Fujisawa, Toshifumi Inada, Yoichi Oda, Shin Takagi
Genes & Development 2008-04-15
Conserved semaphorin-plexin signaling systems govern various aspects of animal development, including axonal guidance in vertebrates and epidermal morphogenesis in Caenorhabditis elegans. Here we provide in vivo evidence that stimulation of mRNA translation via eukaryotic initiation factor 2alpha (eIF2alpha) is an essential down...Read More
Akihisa Kato, Michiko Tanaka, Mayuko Yamamoto, Risa Asai, Tetsutaro Sata, Yukihiro Nishiyama, Yasushi Kawaguchi
Journal of Virology 2008-07-01
Us3 is a serine/threonine protein kinase encoded by herpes simplex virus 1 (HSV-1). Here, we report the identification of a physiological Us3 phosphorylation site on serine at position 147 (Ser-147) which regulates its protein kinase activity in vitro. Moreover, mutation of this site influences Us3 function, including correct lo...Read More
Dariush Mokhtari, Jason W Myers, Nils Welsh
Diabetes 2008-07-01
The transcription factor nuclear factor-kappaB (NF-kappaB) and the mitogen-activated protein kinases (MAPKs) c-Jun NH(2)-terminal kinase (JNK) 1/2 are known to play decisive roles in cytokine-induced damage of rodent beta-cells. The upstream events by which these factors are activated in response to cytokines are, however, uncha...Read More
Kathie M Bishop, Eva K Hofer, Arpesh Mehta, Anthony Ramirez, Liangwu Sun, Mark Tuszynski, Raymond T Bartus
Experimental Neurology 2008-06-01
Treatment of degenerating basal forebrain cholinergic neurons with nerve growth factor (NGF) in Alzheimer's disease has long been contemplated, but an effective and safe delivery method has been lacking. Towards achieving this goal, we are currently developing CERE-110, an adeno-associated virus-based gene delivery vector that e...Read More
Chenghai Dong, Sudarshan C Upadhya, Lan Ding, Thuy K Smith, Ashok N Hegde
Learning & Memory 2008-05-01
Protein degradation by the ubiquitin-proteasome pathway plays important roles in synaptic plasticity, but the molecular mechanisms by which proteolysis regulates synaptic strength are not well understood. We investigated the role of the proteasome in hippocampal late-phase long-term potentiation (L-LTP), a model for enduring syn...Read More
Rajani Srikakulam, Li Liu, Donald A Winkelmann
PLoS ONE 2008-01-01
Myosin folding and assembly in striated muscle is mediated by the general chaperones Hsc70 and Hsp90 and a myosin specific co-chaperone, UNC45. Two UNC45 genes are found in vertebrates, including a striated muscle specific form, Unc45b. We have investigated the role of Unc45b in myosin folding. Epitope tagged murine Unc45b (Unc4...Read More
Sujoy Dutta, Bala Krishna Kolli, Aihua Tang, Shigeru Sassa, Kwang-Poo Chang
Eukaryotic Cell 2008-07-01
Inherent deficiencies of Leishmania in heme biosynthesis were genetically complemented for delta-aminolevulinate-inducible biosynthesis and accumulation of light-excitable uroporphyrin. The phototoxic flagellar immobilization and cytolysis phenotypes and porphyrin mobilization noted previously were further analyzed biochemically...Read More
Lei Jiang, Ge-ning Jiang, Wen-xin He, Jiang Fan, Yi-ming Zhou, Wen Gao, Jia-an Ding
Annals of Thoracic Surgery 2008-06-01
Chronic postoperative empyema remains a challenge for thoracic surgeons. Free musculocutaneous flap transplantation may provide a good alternative option in the treatment of these refractory complications after pulmonary resections. Three patients with chronic postoperative empyemas combined with bronchopleural fistulas underwen...Read More
Manuela Banciu, Raymond M Schiffelers, Gert Storm
Pharmaceutical Research 2008-08-01
Our recent studies show specific localization of long-circulating liposomes (LCL) within the endosomal/lysosomal compartment of tumor-associated macrophages (TAM). Based on this finding, the present study aims to investigate whether clinically applied LCL formulations such as Doxil (LCL-encapsulated doxorubicin), have alternativ...Read More
Sung-Chun Tang, Justin D Lathia, Pradeep K Selvaraj, Dong-Gyu Jo, Mohamed R Mughal, Aiwu Cheng, Dominic A Siler, William R Markesbery, Thiruma V Arumugam, Mark P Mattson
Experimental Neurology 2008-09-01
The innate immune system senses the invasion of pathogenic microorganisms and tissue injury through Toll-like receptors (TLR), a mechanism thought to be limited to immune cells. We recently found that neurons express several TLRs, and that the levels of TLR2 and TLR4 are increased in neurons in response to energy deprivation. He...Read More
Piotr Bebas, Joanna Kotwica, Ewa Joachimiak, Jadwiga M Giebultowicz
BMC Developmental Biology 2008-01-01
Male and female gametes follow diverse developmental pathways dictated by their distinct roles in fertilization. While oocytes of oviparous animals accumulate yolk in the cytoplasm, spermatozoa slough off most of their cytoplasm in the process of individualization. Mammalian spermatozoa released from the testis undergo extensive...Read More
Hanguan Liu, Vincent Manganiello, Nahid Waleh, Ronald I Clyman
Pediatric Research 2008-11-01
A patent ductus arteriosus is due in large part to increased sensitivity of the premature ductus to PGE2. After PGE2 stimulation, cAMP concentrations are higher in the immature than in the mature ductus. cAMP concentrations depend on the rates of adenyl cyclase production and phosphodiesterase (PDE)-mediated degradation. We used...Read More
S R Kim, E Bok, Y C Chung, E S Chung, B K Jin
British Journal of Pharmacology 2008-09-01
We recently proposed the existence of neurotoxic interactions between the cannabinoid type 1 (CB(1)) receptor and transient receptor potential vanilloid 1 (TRPV1) channels in rat mesencephalic cultures. This study seeks evidence for the mediator(s) and mechanisms underlying the neurotoxic interactions between CB(1) receptors and...Read More
Thomas F Tropea, Barry E Kosofsky, Anjali M Rajadhyaksha
Journal of Neurochemistry 2008-08-01
Environment-induced relapse is a major concern in drug addiction because of the strong associations formed between drug reward and environment. Cocaine-conditioned place preference is an ideal experimental tool to examine adaptations in the molecular pathways that are activated upon re-exposure to an environment previously paire...Read More
Olga Danilchanka, Mikhail Pavlenok, Michael Niederweis
Antimicrobial Agents and Chemotherapy 2008-09-01
The outer membrane of mycobacteria presents an effective permeability barrier for many antibiotics. Transport pathways across this membrane are unknown for most drugs. Here, we examined which antibiotics utilize the porin pathway across the outer membrane of the model organism Mycobacterium smegmatis. Deletion of the porins MspA...Read More
Ming-Xiang Zhang, Cheng Zhang, Ying H Shen, Jian Wang, Xiao-Nan Li, Liang Chen, Yun Zhang, Joseph S Coselli, Xing Li Wang
Molecular Biology of the Cell 2008-09-01
We have reported previously that the 27nt repeat polymorphism in endothelial nitric-oxide synthase (eNOS) intron 4--a source of 27nt small RNA--inhibits eNOS expression. In the current study, we have investigated how 27nt small RNA suppresses eNOS expression. Using a chromatin immunoprecipitation assay, we examined histone acety...Read More
Karen Hayton, Deepak Gaur, Anna Liu, Jonathan Takahashi, Bruce Henschen, Subhash Singh, Lynn Lambert, Tetsuya Furuya, Rachel Bouttenot, Michelle Doll, Fatima Nawaz, Jianbing Mu, Lubin Jiang, Louis H Miller, Thomas E Wellems
Cell Host & Microbe 2008-07-17
Some human malaria Plasmodium falciparum parasites, but not others, also cause disease in Aotus monkeys. To identify the basis for this variation, we crossed two clones that differ in Aotus nancymaae virulence and mapped inherited traits of infectivity to erythrocyte invasion by linkage analysis. A major pathway of invasion was ...Read More
Esther J Chen, Erich A Sabio, Sharon R Long
Molecular Microbiology 2008-09-01
Sinorhizobium meliloti requires ExoS/ChvI two-component signalling to establish a nitrogen-fixing symbiosis with legume hosts. The importance of ExoS/ChvI signalling in microbe-host interactions is underscored by the requirement of ExoS/ChvI orthologues for virulence of the related alpha-proteobacteria Agrobacterium tumefaciens ...Read More
Wei Wang, Zong-Qiang Cui, Han Han, Zhi-Ping Zhang, Hong-Ping Wei, Ya-Feng Zhou, Ze Chen, Xian-En Zhang
Nucleic Acids Research 2008-09-01
The mechanisms of influenza A virus mRNA intracellular transport are still not clearly understood. Here, we visualized the distribution and transport of influenza A virus mRNA in living cells using molecular beacon (MB) technology. Confocal-FRAP measurements determined that the transport of influenza A virus intronless mRNA, in ...Read More
R S Spada, R M Cento, C Proto, R Ferri, G Roccasalva, G Toscano, F I Cosentino, A Lanzone
Archives of Gerontology and Geriatrics 2001-01-01
Sonia S Laforce-Nesbitt, Mark A Sullivan, Lois L Hoyer, Joseph M Bliss
FEMS Immunology and Medical Microbiology 2008-11-01
The Candida albicans adhesin, Als3p, was identified as a potential cognate antigen for previously described human antibody fragments [single-chain variable fragment (scFv)] based on similarity of the binding pattern of the scFv to the distribution of this protein on the hyphal surface. Although all scFv bound avidly to wild type...Read More
María Valeria Lara, Sascha Offermann, Monica Smith, Thomas W Okita, Carlos Santiago Andreo, Gerald E Edwards
Plant Physiology 2008-09-01
Bienertia sinuspersici performs C(4) photosynthesis in individual chlorenchyma cells by the development of two cytoplasmic domains (peripheral and central) with dimorphic chloroplasts, an arrangement that spatially separates the fixation of atmospheric CO(2) into C(4) acids and the donation of CO(2) from C(4) acids to Rubisco in...Read More
Wei Wang, Xudong Liao, Koichi Fukuda, Sabine Knappe, Faye Wu, Daniel L Dries, Jun Qin, Qingyu Wu
Circulation Research 2008-08-29
Corin is a cardiac serine protease that acts as the pro-atrial natriuretic peptide (ANP) convertase. Recently, 2 single-nucleotide polymorphisms (SNPs) (T555I and Q568P) in the human corin gene have been identified in genetic epidemiological studies. The minor I555/P568 allele, which is more common in African Americans, is assoc...Read More
Benjamin J Chen, George P Leser, David Jackson, Robert A Lamb
Journal of Virology 2008-10-01
The cytoplasmic tail of the influenza A virus M2 proton-selective ion channel has been shown to be important for virus replication. Previous analysis of M2 cytoplasmic tail truncation mutants demonstrated a defect in incorporation of viral RNA (vRNA) into virions, suggesting a role for M2 in the recruitment of M1-vRNA complexes....Read More
Daniel Sevlever, Peizhou Jiang, Shu-Hui C Yen
Biochemistry (Washington) 2008-09-09
Alpha-synuclein is likely to play a key role in the development of Parkinson's disease as well as other synucleinopathies. In animal models, overexpression of full-length or carboxy-terminally truncated alpha-synuclein has been shown to produce pathology. Although the proteosome and lysosome have been proposed to play a role in ...Read More
Xinhua Zhan, Charles Kim, Frank R Sharp
Brain Research 2008-10-09
This study examined very brief focal ischemia that simulates transient ischemic attacks (TIAs) that occur in humans. Adult rats were subjected to sham operations or 5 min, 10 min, or 2 h of middle cerebral artery (MCA) ischemia using the suture (thread) model. Hsp70 protein was induced 24 h, 48 h and 72 h later in neurons throug...Read More
Nagalingam R Sundaresan, Sadhana A Samant, Vinodkumar B Pillai, Senthilkumar B Rajamohan, Mahesh P Gupta
Molecular and Cellular Biology 2008-10-01
There are seven SIRT isoforms in mammals, with diverse biological functions including gene regulation, metabolism, and apoptosis. Among them, SIRT3 is the only sirtuin whose increased expression has been shown to correlate with an extended life span in humans. In this study, we examined the role of SIRT3 in murine cardiomyocytes...Read More
Naomi D Willis, Thomas R Cox, Syed F Rahman-Casañs, Kim Smits, Stefan A Przyborski, Piet van den Brandt, Manon van Engeland, Matty Weijenberg, Robert G Wilson, Adriaan de Bruïne, Christopher J Hutchison
PLoS ONE 2008-01-01
A-type lamins are type V intermediate filament proteins encoded by the gene LMNA. Mutations in LMNA give rise to diverse degenerative diseases related to premature ageing. A-type lamins also influence the activity of the Retinoblastoma protein (pRb) and oncogenes such a beta-catenin. Consequently, it has been speculated that exp...Read More
Lei Wang, Zhao-Yi Wang
Anticancer Research 2008-07-01
The Wilms' tumor suppressor gene, wt1, encodes a zinc-finger protein, WT1, that functions as a transcription regulator. Previous studies have suggested a contradictory role for WT1 in breast cancer development. MCF10AT3B cells, a cell line derived from a xenograft model of progressive human proliferative breast disease, were use...Read More
Akihiro Naito, Cody C Cook, Takatsugu Mizumachi, Mian Wang, Cheng-Hui Xie, Teresa T Evans, Thomas Kelly, Masahiro Higuchi
Cancer Science 2008-08-01
The growth of LNCaP, a human prostate adenocarcinoma cell line, and MCF-7, a human breast adenocarcinoma cell line, is initially hormone dependent. We previously demonstrated that LNrho0-8 and MCFrho0, derived from LNCaP and MCF-7 by depleting mitochondrial DNA (mtDNA), exhibited hormone-independent growth that led to progressed...Read More
Rocky Pramanik, John R Asplin, Molly E Jackson, James C Williams
Urological Research 2008-10-01
Apatite and brushite kidney stones share calcium and phosphate as their main inorganic components. We tested the hypothesis that these stone types differ in the amount of proteins present in the stones. Intact stones were intensively analyzed by microcomputed tomography (micro CT) for both morphology (including the volume of voi...Read More
Lindsay C Reese, WenRu Zhang, Kelly T Dineley, Rakez Kayed, Giulio Taglialatela
Aging Cell 2008-12-01
Alzheimer's disease (AD) is a terminal age-associated dementia characterized by early synaptic dysfunction and late neurodegeneration. Although the presence of plaques of fibrillar aggregates of the amyloid beta peptide (Abeta) is a signature of AD, evidence suggests that the preplaque small oligomeric Abeta promotes both synapt...Read More
Cishan Li, Wei Huang, Lai-Xi Wang
Bioorganic & Medicinal Chemistry 2008-09-15
A novel application of the Bacillus sp. chitinase for the chemoenzymatic synthesis of N-linked neoglycoproteins is described. Three chitinases with different molecular size were purified from the crude chitinase preparation. The purified chitinases were evaluated for their hydrolytic and transglycosylation activity. One chitinas...Read More
Sandesh Regmi, Karen G Rothberg, James G Hubbard, Larry Ruben
Molecular Microbiology 2008-11-01
RACK1 is a WD-repeat protein that forms signal complexes at appropriate locations in the cell. RACK1 homologues are core components of ribosomes from yeast, plants and mammals. In contrast, a cryo-EM analysis of trypanosome ribosomes failed to detect RACK1, thus eliminating an important translational regulatory mechanism. Here w...Read More
Anilkumar Pillai, Rajalakshmi Veeranan-Karmegam, Krishnan M Dhandapani, Sahebarao P Mahadik
Journal of Neurochemistry 2008-11-01
The role of brain-derived neurotrophic factor (BDNF) has been implicated in the pathophysiology as well as treatment outcome of schizophrenia. Rodent studies indicate that several antipsychotic drugs have time-dependent (and differential) effects on BDNF levels in the brain. Earlier studies from our laboratory have indicated tha...Read More
Wenlan Liu, Rohit Sood, Qingchuan Chen, Unal Sakoglu, Jill Hendren, Ozdemir Cetin, Minoru Miyake, Ke Jian Liu
Journal of Neurochemistry 2008-12-01
Matrix metalloproteinase-9 (MMP-9) and NADPH oxidase contribute to blood-brain barrier (BBB) disruption after ischemic stroke. We have previously shown that normobaric hyperoxia (NBO) treatment reduces MMP-9 and oxygen free radical generation in ischemic brain. In this study, we tested the hypothesis that NBO protects the BBB th...Read More
Samir P Patel, Jorge L Gamboa, Colleen A McMullen, Alexander Rabchevsky, Francisco H Andrade
Investigative Ophthalmology & Visual Science 2009-01-01
The constant activity of the extraocular muscles is supported by abundant mitochondria. These organelles may enhance energy production by increasing the content of respiratory complexes. The authors tested the hypothesis that extraocular muscle mitochondria respire faster than do mitochondria from limb muscles because of the hig...Read More
Aida Maddahi, Lars Edvinsson
BMC Neuroscience 2008-01-01
MEK1/2 is a serine/threonine protein that phosphorylates extracellular signal-regulated kinase (ERK1/2). Cerebral ischemia results in enhanced expression of cerebrovascular contractile receptors in the middle cerebral artery (MCA) leading to the ischemic region. Here we explored the role of the MEK/ERK pathway in receptor expres...Read More
Jun-Yi Yang, Mayumi Iwasaki, Chiyoko Machida, Yasunori Machida, Xueping Zhou, Nam-Hai Chua
Genes & Development 2008-09-15
Viruses induce pathogenic symptoms on plants but the molecular basis is poorly understood. Here, we show that transgenic Arabidopsis expressing the pathogenesis protein betaC1 of Tomato yellow leaf curl China virus (TYLCCNV), a geminivirus, can phenocopy to a large extent disease symptoms of virus-infected tobacco plants in havi...Read More
Balázs Rada, Kristen Lekstrom, Sorin Damian, Corinne Dupuy, Thomas L Leto
Journal of Immunology 2008-10-01
The dual oxidase-thiocyanate-lactoperoxidase (Duox/SCN(-)/LPO) system generates the microbicidal oxidant hypothiocyanite in the airway surface liquid by using LPO, thiocyanate, and Duox-derived hydrogen peroxide released from the apical surface of the airway epithelium. This system is effective against several microorganisms tha...Read More
Winnie W Y Lui-Roberts, Francesco Ferraro, Thomas D Nightingale, Daniel F Cutler
Molecular Biology of the Cell 2008-12-01
Formation of secretory organelles requires the coupling of cargo selection to targeting into the correct exocytic pathway. Although the assembly of regulated secretory granules is driven in part by selective aggregation and retention of content, we recently reported that adaptor protein-1 (AP-1) recruitment of clathrin is essent...Read More
Natalia Louneva, Julia W Cohen, Li-Ying Han, Konrad Talbot, Robert S Wilson, David A Bennett, John Q Trojanowski, Steven E Arnold
The American Journal of Pathology 2008-11-01
Progressive synaptic degeneration and neuron loss are major structural correlates of cognitive impairment in Alzheimer's disease (AD). The mechanisms by which synaptic degeneration in AD occurs have not been established. The activation of proteins within the caspase family has been implicated in AD-associated neurodegeneration, ...Read More
Aleksandra Glogowska, Janette Pyka, Astrid Kehlen, Marek Los, Paul Perumal, Ekkehard Weber, Sheue-yann Cheng, Cuong Hoang-Vu, Thomas Klonisch
Neoplasia 2008-10-01
The intracellular domains of the membrane-anchoring regions of some precursors of epidermal growth factor (EGF) family members have intrinsic biologic activities. We have determined the role of the human proEGF cytoplasmic domain (proEGFcyt) as part of the proEGF transmembrane-anchored region (proEGFctF) in the regulation of mot...Read More
R P McAndrew, J Vockley, J-J P Kim
Journal of Inherited Metabolic Disease 2008-12-01
Dimethylglycine dehydrogenase (DMGDH) is a mitochondrial matrix flavoprotein that catalyses the demethylation of dimethylglycine to form sarcosine, accompanied by the reduction of the covalently bound FAD cofactor. Electron-transfer flavoprotein reoxidizes the reduced flavin and transfers reducing equivalents to the main mitocho...Read More
Yih-Wen Chen, James E Cleaver, Zafer Hatahet, Richard E Honkanen, Jang-Yang Chang, Yun Yen, Kai-Ming Chou
PNAS 2008-10-28
Human DNA polymerase eta (pol eta) can replicate across UV-induced pyrimidine dimers, and defects in the gene encoding pol eta result in a syndrome called xeroderma pigmentosum variant (XP-V). XP-V patients are prone to the development of cancer in sun-exposed areas, and cells derived from XP-V patients demonstrate increased sen...Read More
Ke Yao, Li Zhang, Yidong Zhang, PanPan Ye, Ning Zhu
Molecular Vision 2008-01-01
Ultraviolet (UV) radiation-induced oxidative stress plays a significant role in the progression of cataracts. This study investigated the photoprotective effect of fisetin on UV radiation-induced oxidative stress in human lens epithelial cells and the possible molecular mechanism involved. SRA01/04 cells exposed to different dos...Read More
Fang Liu, Zhong Chen, Jinhong Wang, Xiaofeng Shao, Ziyou Cui, Chunzheng Yang, Zhenping Zhu, Dongsheng Xiong
Neoplasia 2008-11-01
Accumulating evidence suggests that multiple complex mechanisms may be involved, simultaneously or complementarily, in the emergence and development of multidrug resistance (MDR) in various cancers. Cell adhesion-mediated MDR is one such mechanism. In the present study, we initially observed increased cell adhesion to extracellu...Read More
Uri Elia, Eliezer Flescher
Neoplasia 2008-11-01
Methyl jasmonate (MJ) acts both in vitro and in vivo against various cancer cell lines. Activation of the phosphatidylinositol 3-kinase (PI3K)/Akt pathway results in decreased susceptibility to cytotoxic agents in many types of cancer cells. We found a strong inverse correlation between the basal level of phospho-Akt (pAkt) and ...Read More
Chandrashekhar Charavaryamath, Taryn Keet, Gurpreet K Aulakh, Hugh Gg Townsend, Baljit Singh
Journal of Occupational Medicine and Toxicology 2008-01-01
Swine barn air contains endotoxin and many other noxious agents. Single or multiple exposures to pig barn air induces lung inflammation and loss of lung function. However, we do not know the effect of exposure to pig barn air on inflammatory response in the lungs following a secondary infection. Therefore, we tested a hypothesis...Read More
Nameirakpam Dolendro Singh, Ming Li, Sueng-Bum Lee, Danny Schnell, Henry Daniell
Plant Cell 2008-12-01
The chloroplast inner envelope membrane (IM) plays essential roles in lipid synthesis, metabolite transport, and cellular signaling in plants. We have targeted a model nucleus-encoded IM protein from Arabidopsis thaliana, pre-Tic40-His, by relocating its expression from the nucleus to the chloroplast genome. Pre-Tic40-His was pr...Read More
Kavita Bhalla, Yue Luo, Tim Buchan, Michael A Beachem, Gregory F Guzauskas, Sydney Ladd, Shelly J Bratcher, Richard J Schroer, Janne Balsamo, Barbara R DuPont, Jack Lilien, Anand K Srivastava
The American Journal of Human Genetics 2008-12-01
Cell-adhesion molecules play critical roles in brain development, as well as maintaining synaptic structure, function, and plasticity. Here we have found the disruption of two genes encoding putative cell-adhesion molecules, CDH15 (cadherin superfamily) and KIRREL3 (immunoglobulin superfamily), by a chromosomal translocation t(1...Read More
Jia-Chi Chiou, Xiao-Ping Li, Miguel Remacha, Juan P G Ballesta, Nilgun E Tumer
Molecular Microbiology 2008-12-01
Ribosome inactivating proteins (RIPs) like ricin, pokeweed antiviral protein (PAP) and Shiga-like toxins 1 and 2 (Stx1 and Stx2) share the same substrate, the alpha-sarcin/ricin loop, but differ in their specificities towards prokaryotic and eukaryotic ribosomes. Ricin depurinates the eukaryotic ribosomes more efficiently than t...Read More
Changhua Zhou, Ashley M Nitschke, Wei Xiong, Qiang Zhang, Yan Tang, Michael Bloch, Steven Elliott, Yun Zhu, Lindsey Bazzone, David Yu, Christopher B Weldon, Rachel Schiff, John A McLachlan, Barbara S Beckman, Thomas E Wiese, Kenneth P Nephew, Bin Shan, Matthew E Burow, Guangdi Wang
Breast Cancer Research (Online Edition) 2008-01-01
Despite intensive study of the mechanisms of chemotherapeutic drug resistance in human breast cancer, few reports have systematically investigated the mechanisms that underlie resistance to the chemotherapy-sensitizing agent tumor necrosis factor (TNF)-alpha. Additionally, the relationship between TNF-alpha resistance mediated b...Read More
Chun-Yung Huang, Chi-Ming Liang, Chiao-Li Chu, Shu-Mei Liang
BMC Biotechnology 2009-01-01
Numerous proteins can be converted to amyloid-like fibrils to increase cytotoxicity and induce apoptosis, but the methods generally require a high concentration of protein, vigorous shaking, or fibril seed. As well, the detailed mechanism of the cytotoxic effects is not well characterized. In this study, we have developed a nove...Read More
Yan-Qiu Zhang, Zhen Mao, Yuan-Lin Zheng, Bao-Ping Han, Ling-Tong Chen, Jing Li, Fei Li
International Journal of Molecular Sciences 2008-10-01
The present study was performed to investigate the effects of chronic administration of nonylphenol (NP) on the expression of inflammation-related genes in the brains of mice. NP was given orally by gavages at 0, 50, 100, and 200 mg/kg/d. The expression of inflammatory enzymes, inducible nitric oxide synthase (iNOS) and cyclooxy...Read More
D Neves, M Assunção, F Marques, J P Andrade, H Almeida
Age 2008-12-01
Erectile dysfunction (ED) is a highly prevalent disease affecting millions of men worldwide with a tendency for widespread increase. ED is now considered an early manifestation of atherosclerosis and, consequently, a precursor of systemic vascular disease. Atherosclerosis and ED share potentially modifiable risk factors, as smok...Read More
Ling Tian, Nanlan Luo, Richard L Klein, B Hong Chung, W Timothy Garvey, Yuchang Fu
Atherosclerosis 2009-01-01
Adiponectin is one of several, important metabolically active cytokines secreted from adipocytes. Low circulating levels of this adipokine have been associated epidemiologically with obesity, insulin resistance, type II diabetes, and cardiovascular disease. To determine if adiponectin can modulate lipid metabolism in macrophages...Read More
S Armando Villalta, Hal X Nguyen, Bo Deng, Tomomi Gotoh, James G Tidball
Human Molecular Genetics 2009-02-01
Duchenne muscular dystrophy (DMD) is the most common, lethal, muscle-wasting disease of childhood. Previous investigations have shown that muscle macrophages may play an important role in promoting the pathology in the mdx mouse model of DMD. In the present study, we investigate the mechanism through which macrophages promote md...Read More
Ann M Toth, Patricia Devaux, Roberto Cattaneo, Charles E Samuel
Journal of Virology 2009-01-01
The measles virus (MV) accessory proteins V and C play important roles in MV replication and pathogenesis. Infection with recombinant MV lacking either V or C causes more cell death than infection with the parental vaccine-equivalent virus (MVvac), and C-deficient virus grows poorly relative to the parental virus. Here, we show ...Read More
Muthusamy Thangavel, Xiaoqiu Liu, Shu Qiang Sun, Joseph Kaminsky, Rennolds S Ostrom
Cellular Signalling 2009-02-01
Previous data has shown that adenylyl cyclase type 6 (AC6) is expressed principally in lipid rafts or caveolae of cardiac myocytes and other cell types while certain other isoforms of AC are excluded from these microdomains. The mechanism by which AC6 is localized to lipid rafts or caveolae is unknown. In this study, we show AC6...Read More
Y Motomura, H Wang, Y Deng, R T El-Sharkawy, E F Verdu, W I Khan
Clinical and Experimental Immunology 2009-01-01
Inflammatory bowel disease (IBD) is the most common and serious chronic inflammatory condition of the gut. Among the distinct T helper (Th) cell subsets, a Th1 type response is associated predominantly with Crohn's disease (CD) while helminth infections generate a strong Th2 type response. IBD is most prevalent in developed coun...Read More
Melanie J Harriff, Lia Danelishvili, Martin Wu, Cara Wilder, Michael McNamara, Michael L Kent, Luiz E Bermudez
Journal of Bacteriology 2009-02-01
The Mycobacterium avium complex (MAC) is an important group of opportunistic pathogens for birds, cattle, swine, and immunosuppressed humans. Although invasion of epithelial cells lining the intestine is the chief point of entry for these organisms, little is known about the mechanisms by which members of the MAC are taken up by...Read More
Doreen Weidensdorfer, Nadine Stöhr, Anne Baude, Marcell Lederer, Marcel Köhn, Angelika Schierhorn, Sabine Buchmeier, Elmar Wahle, Stefan Hüttelmaier
RNA 2009-01-01
The RNA-binding protein IGF2BP1 (IGF-II mRNA binding protein 1) stabilizes the c-myc RNA by associating with the Coding Region instability Determinant (CRD). If and how other proteins cooperate with IGF2BP1 in promoting stabilization of the c-myc mRNA via the CRD remained elusive. Here, we identify various RNA-binding proteins t...Read More
Alexandra C Silveira, Douglas R Hurst, Kedar S Vaidya, Donald E Ayer, Danny R Welch
Cancer Letters 2009-04-08
BRMS1 and SUDS3 are related members of SIN3-HDAC chromatin remodeling complexes. We hypothesized that they might have overlapping functions and that SUDS3 over-expression could compensate for BRMS1 deficiency. SUDS3 expression was ubiquitous in seven breast cell lines, regardless of metastatic potential. SUDS3 over-expression in...Read More
S C P Costa, P A Girard, M Brehélin, R Zumbihl
Infection and Immunity 2009-03-01
Photorhabdus species are gram-negative entomopathogenic bacteria of the family Enterobacteriaceae. Among the different members of the genus, one species, Photorhabdus asymbiotica, is a pathogen of both insects and humans. The pathogenicity mechanisms of this bacterium are unknown. Here we show that P. asymbiotica is a facultativ...Read More
Ryan G Walker, Andrew Foster, Chris L Randolph, Lori G Isaacson
Brain research 2009-02-19
Mature sympathetic neurons in the superior cervical ganglion (SCG) are regulated by target-derived neurotrophins such as nerve growth factor (NGF) and neurotrophin-3 (NT-3). High molecular weight NGF species and mature NT-3 are the predominant NGF and NT-3 protein isoforms in the SCG, yet it is unknown whether the presence of th...Read More
Thomas S Ream, Jeremy R Haag, Andrzej T Wierzbicki, Carrie D Nicora, Angela D Norbeck, Jian-Kang Zhu, Gretchen Hagen, Thomas J Guilfoyle, Ljiljana Pasa-Tolic, Craig S Pikaard
Molecular Cell 2009-01-30
In addition to RNA polymerases I, II, and III, the essential RNA polymerases present in all eukaryotes, plants have two additional nuclear RNA polymerases, abbreviated as Pol IV and Pol V, that play nonredundant roles in siRNA-directed DNA methylation and gene silencing. We show that Arabidopsis Pol IV and Pol V are composed of ...Read More
Jianmin Zuo, Andrew Currin, Bryan D Griffin, Claire Shannon-Lowe, Wendy A Thomas, Maaike E Ressing, Emmanuel J H J Wiertz, Martin Rowe
PLoS Pathogens 2009-01-01
Epstein-Barr virus (EBV) is a human herpesvirus that persists as a largely subclinical infection in the vast majority of adults worldwide. Recent evidence indicates that an important component of the persistence strategy involves active interference with the MHC class I antigen processing pathway during the lytic replication cyc...Read More
Peter Pelka, Jailal N G Ablack, Joseph Torchia, Andrew S Turnell, Roger J A Grand, Joe S Mymryk
Nucleic Acids Research 2009-03-01
The human adenovirus type 5 (HAdV-5) E1A 13S oncoprotein is a potent regulator of gene expression and is used extensively as a model for transcriptional activation. It possesses two independent transcriptional activation domains located in the N-terminus/conserved region (CR) 1 and CR3. The protein acetyltransferase p300 was pre...Read More
Zhenghe Li, Judit Pogany, Tadas Panavas, Kai Xu, Anthony M Esposito, Terri Goss Kinzy, Peter D Nagy
Virology 2009-03-01
Host RNA-binding proteins are likely to play multiple, integral roles during replication of plus-strand RNA viruses. To identify host proteins that bind to viral RNAs, we took a global approach based on the yeast proteome microarray, which contains 4080 purified yeast proteins. The biotin-labeled RNA probes included two distantl...Read More
Stephanie Abromaitis, P Scott Hefty, Richard S Stephens
FEMS Immunology and Medical Microbiology 2009-03-01
Chlamydia pneumoniae is a community-acquired respiratory pathogen that has been associated with the development of atherosclerosis. Analysis of the C. pneumoniae genome identified a gene (Cpn1046) homologous to eukaryotic aromatic amino acid hydroxylases (AroAA-Hs). AroAA-Hs hydroxylate phenylalanine, tyrosine, and tryptophan in...Read More
Nico Michel, Christine Goffinet, Kerstin Ganter, Ina Allespach, Vineet N Kewalramani, Mohammed Saifuddin, Dan R Littman, Warner C Greene, Mark A Goldsmith, Oliver T Keppler
Retrovirology 2009-01-01
Cells derived from native rodents have limits at distinct steps of HIV replication. Rat primary CD4 T-cells, but not macrophages, display a profound transcriptional deficit that is ameliorated by transient trans-complementation with the human Tat-interacting protein Cyclin T1 (hCycT1). Here, we generated transgenic rats that sel...Read More
Qinghua Zeng, Sharbani Phukan, Yanfei Xu, Maureen Sadim, Diana S Rosman, Michael Pennison, Jie Liao, Guang-Yu Yang, Chiang-Ching Huang, Laura Valle, Antonio Di Cristofano, Albert de la Chapelle, Boris Pasche
Cancer Research 2009-01-15
Transforming growth factor-beta (TGF-beta) signaling is frequently altered in colorectal cancer. Using a novel model of mice heterozygous for a targeted null mutation of Tgfbr1 crossed with Apc(Min/+) mice, we show that Apc(Min/+);Tgfbr1(+/-) mice develop twice as many intestinal tumors as Apc(Min/+);Tgfbr1(+/+) mice, as well as...Read More
Mary Anna Carbone, Julien F Ayroles, Akihiko Yamamoto, Tatiana V Morozova, Steven A West, Michael M Magwire, Trudy F C Mackay, Robert R H Anholt
PLoS ONE 2009-01-01
Glaucoma is the world's second leading cause of bilateral blindness with progressive loss of vision due to retinal ganglion cell death. Myocilin has been associated with congenital glaucoma and 2-4% of primary open angle glaucoma (POAG) cases, but the pathogenic mechanisms remain largely unknown. Among several hypotheses, activa...Read More
Elena Kudryashova, Jun Wu, Leif A Havton, Melissa J Spencer
Human Molecular Genetics 2009-04-01
Limb-girdle muscular dystrophy type 2H (LGMD2H) and sarcotubular myopathy are hereditary skeletal muscle disorders caused by mutations in TRIM32. We previously identified TRIM32 as an E3 ubiquitin ligase that binds to myosin and ubiquitinates actin. To date four TRIM32 mutations have been linked to LGMD2H, all of which occur in ...Read More
Timo Glatter, Alexander Wepf, Ruedi Aebersold, Matthias Gstaiger
Molecular Systems Biology 2009-01-01
Protein complexes represent major functional units for the execution of biological processes. Systematic affinity purification coupled with mass spectrometry (AP-MS) yielded a wealth of information on the compendium of protein complexes expressed in Saccharomyces cerevisiae. However, global AP-MS analysis of human protein comple...Read More
Shiho Tanaka, Michael R Sawaya, Martin Phillips, Todd O Yeates
Protein Science 2009-01-01
Carboxysomes are primitive bacterial organelles that function as a part of a carbon concentrating mechanism (CCM) under conditions where inorganic carbon is limiting. The carboxysome enhances the efficiency of cellular carbon fixation by encapsulating together carbonic anhydrase and the CO(2)-fixing enzyme ribulose-1,5-bisphosph...Read More
Miaozong Wu, Devashish H Desai, Sunil K Kakarla, Anjaiah Katta, Satyanarayana Paturi, Anil K Gutta, Kevin M Rice, Ernest M Walker, Eric R Blough
Diabetes - Metabolism: Research and Reviews (Print Edition) 2009-03-01
Aging-related hyperglycemia is associated with increased oxidative stress and diminished muscle glucose transporter-4 (Glut4) that may be regulated, at least in part, by the mitogen-activated protein kinases (MAPK). To test the possibility that aging-related hyperglycemia can be prevented by pharmacological manipulation of MAPK ...Read More
Kathleen A O'Hagan, Sinead Cocchiglia, Alexander V Zhdanov, Murtaza M Tambuwala, Murtaza M Tambawala, Eoin P Cummins, Mona Monfared, Terence A Agbor, John F Garvey, Dmitri B Papkovsky, Cormac T Taylor, Bernard B Allan
PNAS 2009-02-17
Mitochondrial biogenesis occurs in response to increased cellular ATP demand. The mitochondrial electron transport chain requires molecular oxygen to produce ATP. Thus, increased ATP generation after mitochondrial biogenesis results in increased oxygen demand that must be matched by a corresponding increase in oxygen supply. We ...Read More
Masahiro Nomoto, Masaaki Miyata, Shanai Yin, Yasushi Kurata, Miki Shimada, Kouichi Yoshinari, Frank J Gonzalez, Kokichi Suzuki, Shigeki Shibasaki, Tohru Kurosawa, Yasushi Yamazoe
Biological & Pharmaceutical Bulletin 2009-02-01
The major function of farnesoid X receptor (FXR) is to maintain bile acid and lipid homeostasis. Fxr-null mice, in which the levels of hepatic bile acid and lipid have been elevated, develop spontaneous liver tumors. We evaluated differences in hepatic bile acid and triglyceride concentrations, and in generation of oxidative str...Read More
María P Sánchez-Cañete, Luís Carvalho, F Javier Pérez-Victoria, Francisco Gamarro, Santiago Castanys
Antimicrobial Agents and Chemotherapy 2009-04-01
Miltefosine (hexadecylphosphocholine, MLF) is the first oral drug with recognized efficacy against both visceral and cutaneous leishmaniasis. However, some clinical studies have suggested that MLF shows significantly less efficiency against the cutaneous leishmaniasis caused by Leishmania braziliensis. In this work, we have dete...Read More
Zhen Zhang, Deanne Alpert, Richard Francis, Bishwanath Chatterjee, Qing Yu, Terry Tansey, Steven L Sabol, Cheng Cui, Yongli Bai, Maxim Koriabine, Yuko Yoshinaga, Jan-Fang Cheng, Feng Chen, Joel Martin, Wendy Schackwitz, Teresa M Gunn, Kenneth L Kramer, Pieter J De Jong, Len A Pennacchio, Cecilia W Lo
PNAS 2009-03-03
Forward genetic screens with ENU (N-ethyl-N-nitrosourea) mutagenesis can facilitate gene discovery, but mutation identification is often difficult. We present the first study in which an ENU-induced mutation was identified by massively parallel DNA sequencing. This mutation causes heterotaxy and complex congenital heart defects ...Read More
Lijian Shao, Wei Feng, Yan Sun, Hao Bai, Jun Liu, Caroline Currie, Jaejung Kim, Rafael Gama, Zack Wang, Zhijian Qian, Lucy Liaw, Wen-Shu Wu
Cell Research 2009-03-01
Generation of induced pluripotent stem (iPS) cells from somatic cells has been achieved successfully by simultaneous viral transduction of defined reprogramming transcription factors (TFs). However, the process requires multiple viral vectors for gene delivery. As a result, generated iPS cells harbor numerous viral integration s...Read More
Robert Salomon, Lei Young, Duncan Macleod, Xiao-Ling Yu, Qihan Dong
Journal of Histochemistry & Cytochemistry 2009-06-01
Inhibitor of DNA-binding-1 (ID1) negatively regulates cell differentiation and senescence, and enhances cellular proliferation and angiogenesis. Elevated levels of ID1 have been found in a variety of cancers, including prostate cancer, but whether ID1 has a tumourigenic role remains to be established. We established heterozygous...Read More
Maria Lodeiro, Marily Theodoropoulou, Maria Pardo, Felipe F Casanueva, Jesus P Camiña
PLoS ONE 2009-01-01
The aim of the present study was to identify the signaling mechanisms to ghrelin-stimulated activation of the serine/threonine kinase Akt. In human embryonic kidney 293 (HEK293) cells transfected with GHS-R1a, ghrelin leads to the activation of Akt through the interplay of distinct signaling mechanisms: an early G(i/o) protein-d...Read More
Stacey N Williams, Ashiwel S Undieh
Neuroreport 2009-04-22
Recent studies showed that dopamine or D1 receptor-selective agonists increased brain-derived neurotrophic factor (BDNF) mRNA and protein expression in neuronal cultures, and this action was blocked by SCH23390. Moreover, SKF38393 activated Trk receptors and downstream signaling in striatal neurons. This study examined whether d...Read More
N F Bello, I Lamsoul, M L Heuzé, A Métais, G Moreaux, D A Calderwood, D Duprez, C Moog-Lutz, P G Lutz
Cell Death and Differentiation 2009-06-01
Ubiquitin-mediated protein degradation is the main mechanism for controlled proteolysis, which is crucial for muscle development and maintenance. The ankyrin repeat-containing protein with a suppressor of cytokine signaling box 2 gene (ASB2) encodes the specificity subunit of an E3 ubiquitin ligase complex involved in differenti...Read More
Kyungtae Kim, Sung Chun Cho, Anthony Cova, Ik Soon Jang, Sang Chul Park
Experimental & Molecular Medicine 2009-05-31
The effects of glucagon and epinephrine on gluconeogenesis in young (4 month) and old (24 month) Fisher 344 rat hepatocytes were compared. In contrast to glucagon, which had a similar effect on gluconeogenesis in both young and old cells, epinephrine caused a smaller increase in gluconeogenesis in old rat hepatocytes than in you...Read More
Ping Zhang, Jeffrey O Langland, Bertram L Jacobs, Charles E Samuel
Journal of Virology 2009-06-01
The p38 and c-Jun N-terminal kinase (JNK) mitogen-activated protein kinases (MAPKs) play important roles in the host innate immune response. The protein kinase regulated by RNA (PKR) is implicated in p38 MAPK activation in response to proinflammatory signals in mouse embryonic fibroblasts. To test the role of PKR in the activati...Read More
Esther Yakir, Dror Hilman, Ido Kron, Miriam Hassidim, Naomi Melamed-Book, Rachel M Green
Plant Physiology 2009-06-01
As an adaptation to life in a world with predictable daily changes, most eukaryotes and some prokaryotes have endogenous circadian (approximately 24 h) clocks. In plants, the circadian clock regulates a diverse range of cellular and physiological events from gene expression and protein phosphorylation to cellular calcium oscilla...Read More
Phattara-Orn Havanapan, Visith Thongboonkerd
Journal of Proteome Research 2009-06-01
Proteolysis is one of the major problems in collection and storage of biological samples for proteome analysis, particularly when the samples undergo freeze-thaw cycles. The use of protease inhibitors for prevention of such proteolysis in some samples is debated because protease inhibitors may interfere with proteome analysis an...Read More
Kristen Pollizzi, Izabela Malinowska-Kolodziej, Cheryl Doughty, Charles Betz, Jian Ma, June Goto, David J Kwiatkowski
Human Molecular Genetics 2009-07-01
Tuberous sclerosis complex (TSC) is a tumor suppressor gene syndrome in which hamartomas develop in multiple organ systems. Knockout and conditional alleles of Tsc1 and Tsc2 have been previously reported. Here, we describe the generation of a novel hypomorphic allele of Tsc2 (del3), in which exon 3, encoding 37 amino acids near ...Read More
Brian D Lamon, Barbara D Summers, Antonio M Gotto, David P Hajjar
European Journal of Pharmacology 2009-03-15
Statins have been demonstrated to elicit a broad range of cellular events resulting in an attenuation of the inflammatory response and enhanced protection to the components of the vessel wall. The present study was designed to examine the effect of pitavastatin on pathways associated with the proinflammatory gene, early growth r...Read More
Courtney G Woods, Jingqi Fu, Peng Xue, Yongyong Hou, Linda J Pluta, Longlong Yang, Qiang Zhang, Russell S Thomas, Melvin E Andersen, Jingbo Pi
Toxicology and Applied Pharmacology 2009-07-01
Hypochlorous acid (HOCl) is potentially an important source of cellular oxidative stress. Human HOCl exposure can occur from chlorine gas inhalation or from endogenous sources of HOCl, such as respiratory burst by phagocytes. Transcription factor Nrf2 is a key regulator of cellular redox status and serves as a primary source of ...Read More
Suzanne Paz, Myriam Vilasco, Meztli Arguello, Qiang Sun, Judith Lacoste, Thi Lien-Anh Nguyen, Tiejun Zhao, Elena A Shestakova, Scott Zaari, Annie Bibeau-Poirier, Marc J Servant, Rongtuan Lin, Eliane F Meurs, John Hiscott
Molecular and Cellular Biology 2009-06-01
Induction of the antiviral interferon response is initiated upon recognition of viral RNA structures by the RIG-I or Mda-5 DEX(D/H) helicases. A complex signaling cascade then converges at the mitochondrial adapter MAVS, culminating in the activation of the IRF and NF-kappaB transcription factors and the induction of interferon ...Read More
Lucila Sackmann-Sala, Juan Ding, Lawrence A Frohman, John J Kopchick
Growth Hormone & IGF Research 2009-12-01
To identify biomarkers of growth hormone (GH) and insulin-like growth factor 1 (IGF-1) action in human serum. The search for new markers of GH activity has received extensive attention given that the current biomarkers (IGF-1, IGFBP-3 and collagen peptides) show substantial variability in the population, and are not reliably pre...Read More
Jill S Butler, Lakshmi R Palam, Courtney M Tate, Jeremy R Sanford, Ronald C Wek, David G Skalnik
DNA and Cell Biology 2009-05-01
CXXC finger protein 1 (CFP1) binds to unmethylated CpG dinucleotides and is required for embryogenesis. CFP1 is also a component of the Setd1A and Setd1B histone H3K4 methyltransferase complexes. Murine embryonic stem (ES) cells lacking CFP1 fail to differentiate, and exhibit a 70% reduction in global genomic cytosine methylatio...Read More
Roman Szabo, Peter Kosa, Karin List, Thomas H Bugge
The American Journal of Pathology 2009-06-01
Hepatocyte growth factor activator inhibitor-1 (HAI)-1 is an epithelial Kunitz-type transmembrane serine protease inhibitor that is encoded by the SPINT1 gene. HAI-1 displays potent inhibitory activity toward a large number of trypsin-like serine proteases. HAI-1 was recently shown to play an essential role in postnatal epitheli...Read More
Anthony G Pajerowski, Chau Nguyen, Haig Aghajanian, Michael J Shapiro, Virginia Smith Shapiro
Immunity 2009-05-01
T cell development depends on the coordinated interplay between receptor signaling and transcriptional regulation. Through a genetic complementation screen a transcriptional repressor, NKAP, was identified. NKAP associated with the histone deacetylase HDAC3 and was shown to be part of a DNA-binding complex, as demonstrated by ch...Read More
W Michael Caudle, Efstathia Kitsou, Jane Li, Joshua Bradner, Jing Zhang
Neuroscience Letters 2009-07-31
Although much has been learned in the last few decades concerning the molecular mechanisms and pathways associated with the development of familial as well as sporadic Parkinson disease (PD), the precise mechanisms and specific proteins responsible for mediating these effects remain to be elucidated. Thus, the identification and...Read More
Mariko Saito, Goutam Chakraborty, Rui-Fen Mao, Csaba Vadasz, Mitsuo Saito
Neurochemical Research 2009-11-01
It has been shown that lipogenic enzymes, such as fatty acid synthase (FAS) and acetyl-CoA carboxylase (ACC), are highly expressed in the rodent brain during the early neonatal period and decline thereafter. However, cellular localization of these enzymes is unknown. Presently, we examined developmental changes in the levels and...Read More
Laurie A Robak, Karthik Venkatesh, Hakjoo Lee, Stephen J Raiker, Yuntao Duan, Jane Lee-Osbourne, Thomas Hofer, Rose G Mage, Christoph Rader, Roman J Giger
Journal of Neuroscience 2009-05-06
Myelin-associated glycoprotein (MAG) is a sialic acid-binding Ig-family lectin that functions in neuronal growth inhibition and stabilization of axon-glia interactions. The ectodomain of MAG is comprised of five Ig-like domains and uses neuronal cell-type-specific mechanisms to signal growth inhibition. We show that the first th...Read More
Xiao-Jun Wang, Donna D Zhang
PLoS ONE 2009-01-01
The transcription factor Nrf2 is the master regulator of a cellular defense mechanism against environmental insults. The Nrf2-mediated antioxidant response is accomplished by the transcription of a battery of genes that encode phase II detoxifying enzymes, xenobiotic transporters, and antioxidants. Coordinated expression of thes...Read More
Dariusz Abramczyk, Paul J Szaniszlo
Preparative Biochemistry and Biotechnology 2009-01-01
The class V chitin synthase is unique because it has a myosin motor-like domain fused to its catalytic domain. The biochemical properties of this enzyme and its function remain undefined beyond the knowledge that it is the only single chitin synthase required for sustained cell growth at elevated temperatures and, consequently, ...Read More
Shensi Shen, Pengtao Zhang, Martin A Lovchik, Ying Li, Liuya Tang, Zhimin Chen, Rong Zeng, Dawei Ma, Junying Yuan, Qiang Yu
Journal of Cell Biology 2009-05-18
Promoting the degradation of Hsp90 client proteins by inhibiting Hsp90, an important protein chaperone, has been shown to be a promising new anticancer strategy. In this study, we show that an oxazoline analogue of apratoxin A (oz-apraA), a cyclodepsipeptide isolated from a marine cyanobacterium, promotes the degradation of Hsp9...Read More
Arnaud Ahier, Philippe Rondard, Nadège Gouignard, Naji Khayath, Siluo Huang, Jacques Trolet, Daniel J Donoghue, Monique Gauthier, Jean-Philippe Pin, Colette Dissous
PLoS ONE 2009-01-01
Tyrosine kinase receptors (RTKs) comprise a large family of membrane receptors that regulate various cellular processes in cell biology of diverse organisms. We previously described an atypical RTK in the platyhelminth parasite Schistosoma mansoni, composed of an extracellular Venus flytrap module (VFT) linked through a single t...Read More
Matthew P Badtke, Irfan Khan, Feng Cao, Jianming Hu, John E Tavis
Virology 2009-07-20
The T3 motif on the duck hepatitis B virus reverse transcriptase (P) is proposed to be a binding site essential for viral replication, but its ligand and roles in DNA synthesis are unknown. Here, we found that T3 is needed for P to bind the viral RNA, the first step in DNA synthesis. A second motif, RT-1, was predicted to assist...Read More
A L Mandel, H Ozdener, V Utermohlen
Archives of Oral Biology 2009-07-01
Growth factors, including brain-derived neurotrophic factor (BDNF), are polypeptides that are involved in the maintenance, survival, and death of central and peripheral cells. Numerous growth factors have been identified in saliva and are thought to promote wound healing and maintenance of the oral epithelium. The aim of this st...Read More
Senthilkumar B Rajamohan, Vinodkumar B Pillai, Madhu Gupta, Nagalingam R Sundaresan, Konstantin G Birukov, Sadhana Samant, Michael O Hottiger, Mahesh P Gupta
Molecular and Cellular Biology 2009-08-01
Poly(ADP-ribose) polymerase 1 (PARP1) and SIRT1 deacetylase are two NAD-dependent enzymes which play major roles in the decision of a cell to live or to die in a stress situation. Because of the dependence of both enzymes on NAD, cross talk between them has been suggested. Here, we show that PARP1 is acetylated after stress of c...Read More
Narender Nath, Musfiquidin Khan, Manjeet K Paintlia, Inderjit Singh, Md Nasrul Hoda, Shailendra Giri
Journal of Immunology 2009-06-15
Experimental autoimmune encephalomyelitis (EAE) is a T cell-mediated autoimmune disease of the CNS. Metformin is the most widely used drug for diabetes and mediates its action via activating AMP-activated protein kinase (AMPK). We provide evidence that metformin attenuates the induction of EAE by restricting the infiltration of ...Read More
Animesh Barua, Seby L Edassery, Pincas Bitterman, Jacques S Abramowicz, Angela L Dirks, Janice M Bahr, Dale B Hales, Michael J Bradaric, Judith L Luborsky
International Journal of Gynecological Cancer 2009-05-01
Antitumor antibodies are associated with tumors in human cancers. There is relatively little information on the timing and progression of antibody response to tumors. The objective of the study was to determine if spontaneous ovarian cancer in the egg-laying hen is associated with antitumor antibodies. Antibodies were detected b...Read More
Paul Curtin, Kevin P McHugh, Hai-Yan Zhou, Rudolf Flückiger, Paul Goldhaber, Frank G Oppenheim, Erdjan Salih
Biochemistry (Washington) 2009-07-28
We have determined transmembrane protein tyrosine phosphorylation (outside-in signaling) in cultured osteoclasts and macrophages in response to added native purified bone sialoprotein (nBSP) and its dephosphorylated form (dBSP). There were selective/differential and potent inhibitory effects by dBSP and minimal effect by nBSP on...Read More
Wendy Ann Peer, Fazeeda N Hosein, Anindita Bandyopadhyay, Srinivas N Makam, Marisa S Otegui, Gil-Je Lee, Joshua J Blakeslee, Yan Cheng, Boosaree Titapiwatanakun, Bahktiyor Yakubov, Bharat Bangari, Angus S Murphy
Plant Cell 2009-06-01
Aminopeptidase M1 (APM1), a single copy gene in Arabidopsis thaliana, encodes a metallopeptidase originally identified via its affinity for, and hydrolysis of, the auxin transport inhibitor 1-naphthylphthalamic acid (NPA). Mutations in this gene result in haploinsufficiency. Loss-of-function mutants show irregular, uncoordinated...Read More
A J Ryan, S Napoletano, P A Fitzpatrick, C A Currid, N C O'Sullivan, J H Harmey
British Journal of Cancer 2009-07-21
Insulin-like growth factor 1 (IGF1) promotes breast cancer and disease progression. Bioavailability of IGF1 is modulated by IGF-binding proteins (IGFBPs). IGFBP4 inhibits IGF1 activity but cleavage by pregnancy-associated plasma protein-A (PAPP-A) protease releases active IGF1. Expression of IGF pathway components and PAPP-A was...Read More
S A Follows, J Murlidharan, P Massari, L M Wetzler, C A Genco
Infection and Immunity 2009-09-01
Several microbial pathogens can modulate the host apoptotic response to infection, which may contribute to immune evasion. Various studies have reported that infection with the sexually transmitted disease pathogen Neisseria gonorrhoeae can either inhibit or induce apoptosis. N. gonorrhoeae infection initiates at the mucosal epi...Read More
Travis W Day, Ching-Huang Wu, Ahmad R Safa
Molecular Pharmacology 2009-09-01
In this report, we reveal that etoposide inhibits the proliferation of SK-N-AS neuroblastoma cancer cells and promotes protein kinase Cdelta (PKCdelta)- and caspase-dependent apoptosis. Etoposide induces the caspase-3-dependent cleavage of PKCdelta to its active p40 fragment, and active PKCdelta triggers the processing of caspas...Read More
Guangli Suo, Yong Jiang, Bryan Cowan, Jean Y J Wang
Biology of Reproduction 2009-10-01
Leiomyomata uteri (i.e., uterine fibroids) are benign tumors arising from the abnormal growth of uterine smooth muscle cells (SMCs). We show here that the expression of platelet-derived growth factor C (PDGFC) is higher in approximately 80% of uterine fibroids than in adjacent myometrial tissues examined. Increased expression of...Read More
Lorenza Eder-Colli, Noelia Abad-Estarlich, Carine Pannetier, Philippe G Vallet, Claude Walzer, Gregory A Elder, Nikolaos K Robakis, Constantin Bouras, Armand Savioz
Brain Research Bulletin 2009-10-28
The presenilin-1 gene is mutated in early-onset familial Alzheimer's disease. The mutation Pro117Leu is implicated in a very severe form of the disease, with an onset of less than 30 years. The consequences of this mutation on neurogenesis in the hippocampus of adult transgenic mice have already been studied in situ. The surviva...Read More
Lisa E Gralinski, Shanna L Ashley, Shandee D Dixon, Katherine R Spindler
Journal of Virology 2009-09-01
Infection with mouse adenovirus type 1 (MAV-1) results in fatal acute encephalomyelitis in susceptible mouse strains via infection of brain endothelial cells. Wild-type (wt) MAV-1 causes less brain inflammation than an early region 3 (E3) null virus in C57BL/6 mice. A mouse brain microvascular endothelial cell line infected with...Read More
Qing-wen Chen, Lars Edvinsson, Cang-Bao Xu
BMC Cell Biology 2009-01-01
muscle cells (VSMCs) through activation of endothelin type A (ETA) and type B (ETB) receptors. The extracellular signal-regulated kinase 1 and 2 (ERK1/2) mitogen-activated protein kinases (MAPK) are involved in ET-1-induced VSMC contraction and proliferation. This study was designed to investigate the ETA and ETB receptor intrac...Read More
Syng-Ook Lee, Sudhakar Chintharlapalli, Shengxi Liu, Sabitha Papineni, Sung Dae Cho, Kyungsil Yoon, Stephen Safe
Molecular Cancer Research 2009-07-01
1,1-Bis(3'-indolyl)-1-(p-anisyl)methane (DIM-C-pPhOCH3) activates the orphan receptor nerve growth factor-induced Balpha (Nur77) in cancer cells, and in this study, DIM-C-pPhOCH3 decreased Panc1 pancreatic cancer cell survival and arrested cells in G0-G1. These responses were accompanied by induction of the cyclin-dependent kina...Read More
Subbiah Pugazhenthi, Donald H Gilden, Sreekala Nair, Anne McAdoo, Mary Wellish, Elizabeth Brazeau, Ravi Mahalingam
Journal of Virology 2009-09-01
Simian varicella virus (SVV) causes varicella in primates, becomes latent in ganglionic neurons, and reactivates to produce zoster. SVV produces a cytopathic effect in monkey kidney cells in tissue culture. To study the mechanism by which SVV-infected cells die, we examined markers of apoptosis 24 to 64 h postinfection (hpi). We...Read More
Junxia Tang, Robert P LeGros, Natalia Louneva, Lilly Yeh, Julia W Cohen, Chang-Gyu Hahn, Derek J Blake, Steven E Arnold, Konrad Talbot
Human Molecular Genetics 2009-10-15
DTNBP1 (dystrobrevin binding protein 1) remains a top candidate gene in schizophrenia. Reduced expression of this gene and of its encoded protein, dysbindin-1, have been reported in the brains of schizophrenia cases. It has not been established, however, if the protein reductions encompass all dysbindin-1 isoforms or if they are...Read More
Huaxin Sheng, Wei Yang, Shiro Fukuda, Hubert M Tse, Wulf Paschen, Kwame Johnson, Ines Batinic-Haberle, James D Crapo, Robert D Pearlstein, Jon Piganelli, David S Warner
Free Radical Biology and Medicine 2009-10-01
Sustained oxidative stress is a known sequel to focal cerebral ischemia. This study examined the effects of treatment with a single dose or sustained infusion of the redox-modulating MnPorphyrin Mn(III)TDE-2-ImP(5+) on outcome from middle cerebral artery occlusion (MCAO) in the rat. Normothermic rats were subjected to 90 min MCA...Read More
Virginie Bottero, Neelam Sharma-Walia, Nagaraj Kerur, Arun George Paul, Sathish Sadagopan, Mark Cannon, Bala Chandran
Virology 2009-09-15
KSHV vGPCR, a lytic cycle associated protein, induces several signaling pathways leading to the activation of various transcription factors and consequently the expression of cellular and viral genes. Though the role of vGPCR in KSHV tumorigenicity has been well studied, its function related to the viral life cycle is poorly und...Read More
Miaozong Wu, Anjaiah Katta, Murali K Gadde, Hua Liu, Sunil K Kakarla, Jacqueline Fannin, Satyanarayana Paturi, Ravi K Arvapalli, Kevin M Rice, Yeling Wang, Eric R Blough
PLoS ONE 2009-01-01
Aged skeletal muscle is characterized by an increased incidence of metabolic and functional disorders, which if allowed to proceed unchecked can lead to increased morbidity and mortality. The mechanism(s) underlying the development of these disorders in aging skeletal muscle are not well understood. Protein kinase B (Akt/PKB) is...Read More
Yuhui Wen, Yuanyuan Xu, Yingcai Wang, Jose Renato Pinto, James D Potter, W Glenn L Kerrick
Journal of Molecular Biology 2009-10-09
The human cardiac troponin I (hcTnI) mutation R145W has been associated with restrictive cardiomyopathy. In this study, simultaneous measurements of ATPase activity and force in skinned papillary fibers from hcTnI R145W transgenic mice (Tg-R145W) were explored. Tg-R145W fibers showed an approximately 13-16% increase in maximal C...Read More
Junjie Hu, Yoko Shibata, Peng-Peng Zhu, Christiane Voss, Neggy Rismanchi, William A Prinz, Tom A Rapoport, Craig Blackstone
Cell 2009-08-07
The endoplasmic reticulum (ER) consists of tubules that are shaped by the reticulons and DP1/Yop1p, but how the tubules form an interconnected network is unknown. Here, we show that mammalian atlastins, which are dynamin-like, integral membrane GTPases, interact with the tubule-shaping proteins. The atlastins localize to the tub...Read More
Maria B Koenigs, Elizabeth A Richardson, Danielle H Dube
Molecular BioSystems 2009-09-01
Metabolic oligosaccharide engineering was used to profile glycoproteins of the human pathogen Helicobacter pylori.Read More
Holger Stephan, Claire Concannon, Elisabeth Kremmer, Michael P Carty, Heinz-Peter Nasheuer
Nucleic Acids Research 2009-10-01
The human single-stranded DNA-binding protein, replication protein A (RPA), is regulated by the N-terminal phosphorylation of its 32-kDa subunit, RPA2. RPA2 is hyperphosphorylated in response to various DNA-damaging agents and also phosphorylated in a cell-cycle-dependent manner during S- and M-phase, primarily at two CDK consen...Read More
Nigel A Calcutt, Veronica L Lopez, Arjel D Bautista, Leah M Mizisin, Brenda R Torres, Albert L Shroads, Andrew P Mizisin, Peter W Stacpoole
Journal of Neuropathology & Experimental Neurology 2009-09-01
The use of dichloroacetate (DCA) for treating patients with mitochondrial diseases is limited by the induction of peripheral neuropathy. The mechanisms of DCA-induced neuropathy are not known. Oral DCA treatment (50-500 mg/kg per day for up to 16 weeks) induced tactile allodynia in both juvenile and adult rats; concurrent therma...Read More
Karine Loulier, Justin D Lathia, Veronique Marthiens, Jenne Relucio, Mohamed R Mughal, Sung-Chun Tang, Turhan Coksaygan, Peter E Hall, Srinivasulu Chigurupati, Bruce Patton, Holly Colognato, Mahendra S Rao, Mark P Mattson, Tarik F Haydar, Charles Ffrench-Constant
PLoS Biology 2009-08-01
During embryogenesis, the neural stem cells (NSC) of the developing cerebral cortex are located in the ventricular zone (VZ) lining the cerebral ventricles. They exhibit apical and basal processes that contact the ventricular surface and the pial basement membrane, respectively. This unique architecture is important for VZ physi...Read More
Peng Yue, Dao-Hong Lin, Chun-Yang Pan, Qiang Leng, Gerhard Giebisch, Richard P Lifton, Wen-Hui Wang
PNAS 2009-09-01
WNK4 (with no lysine kinase 4) inhibits ROMK channel activity in the distal nephron by stimulating clathrin-dependent endocytosis, an effect attenuated by SGK1 (serum-glucocorticoids-induced kinase)-mediated phosphorylation. It has been suggested that increased ROMK activity because of SGK1-mediated inhibition of WNK4 plays a ro...Read More
Hae-Seong Nam, Sook Young Lee, Seung Jun Kim, Ju Sang Kim, Soon Seog Kwon, Young Kyoon Kim, Kwan Hyung Kim, Hwa Sik Moon, Jeong Sup Song, Sung Hak Park, Seok Chan Kim
Yonsei Medical Journal 2009-08-31
Tumor necrosis factor-alpha (TNF-alpha) is a proinflammatory cytokine that has been implicated in many aspects of the airway pathology in asthma. TNF-alpha blocking strategies are now being tried in asthma patients. This study investigated whether TNF-alpha blocking therapy inhibits airway inflammation and airway hyperresponsive...Read More
Luis A Estrella, Miles F Wilkinson, Carlos I González
Journal of Molecular Biology 2009-12-04
Heterogeneous nuclear ribonucleoproteins are multifunctional proteins that bind to newly synthesized mRNAs in the nucleus and participate in many subsequent steps of gene expression. A well-studied Saccharomyces cerevisiae heterogeneous nuclear ribonucleoprotein that has several nuclear functions is Npl3p. Here, we provide evide...Read More
Phyllis S Frisa, James W Jacobberger
PLoS ONE 2009-01-01
To obtain non-relative measures of cell proteins, purified preparations of the same proteins are used as standards in Western blots. We have previously quantified SV40 large T antigen expressed over a several fold range in different cell lines and correlated the average number of molecules to average fluorescence obtained by cyt...Read More
Miklos Guttman, Gina N Betts, Helen Barnes, Majid Ghassemian, Peter van der Geer, Elizabeth A Komives
Proteomics 2009-11-01
The low density lipoprotein receptor-related protein 1 (LRP1) mediates internalization of a large number of proteins and protein-lipid complexes and is widely implicated in Alzheimer's disease. The cytoplasmic domain of LRP1 (LRP1-CT) can be phosphorylated by activated protein-tyrosine kinases at two NPXY motifs in LRP1-CT; Tyr ...Read More
Huifen Zhu, Guo-Jing Li, Lei Ding, Xiangqin Cui, Howard Berg, Sarah M Assmann, Yiji Xia
Molecular Plant 2009-05-01
Heterotrimeric GTP-binding proteins, which consist of Galpha, Gbeta, and Ggamma subunits, play important roles in transducing extracellular signals perceived by cell surface receptors into intracellular physiological responses. In addition to a single prototypical Galpha protein (GPA1), Arabidopsis has three unique Galpha-like p...Read More
Yukiko Doi, Tetsuya Mizuno, Yuki Maki, Shijie Jin, Hiroyuki Mizoguchi, Masayoshi Ikeyama, Minoru Doi, Makoto Michikawa, Hideyuki Takeuchi, Akio Suzumura
The American Journal of Pathology 2009-11-01
Soluble oligomeric amyloid beta (oAbeta) 1-42 causes synaptic dysfunction and neuronal injury in Alzheimer's disease (AD). Although accumulation of microglia around senile plaques is a hallmark of AD pathology, the role of microglia in oAbeta1-42 neurotoxicity is not fully understood. Here, we showed that oAbeta but not fibrilla...Read More
Feroz Sarkari, Teresa Sanchez-Alcaraz, Shan Wang, Melissa N Holowaty, Yi Sheng, Lori Frappier
PLoS Pathogens 2009-10-01
The EBNA1 protein of Epstein-Barr virus (EBV) plays essential roles in enabling the replication and persistence of EBV genomes in latently infected cells and activating EBV latent gene expression, in all cases by binding to specific recognition sites in the latent origin of replication, oriP. Here we show that EBNA1 binding to i...Read More
Yang Wang, Yoshiaki Azuma, David B Friedman, Robert J Coffey, Kristi L Neufeld
BMC Cell Biology 2009-01-01
As a key player in suppression of colon tumorigenesis, Adenomatous Polyposis Coli (APC) has been widely studied to determine its cellular functions. However, inconsistencies of commercially available APC antibodies have limited the exploration of APC function. APC is implicated in spindle formation by direct interactions with tu...Read More
Yang Su, Gang Deng, Yuanming Gai, Yue Li, Yang Gao, Jiansen Du, Yunqi Geng, Qimin Chen, Wentao Qiao
Virology Journal 2009-01-01
Jembrana disease virus (JDV) encodes a potent regulatory protein Tat that strongly stimulates viral expression by transactivating the long terminal repeat (LTR) promoter. JDV Tat (jTat) promotes the transcription from its own LTR as well as non-cognate LTRs, by recruiting host transcription factors and facilitating transcription...Read More
Larry E Jones, Lei Ying, Anne B Hofseth, Elena Jelezcova, Robert W Sobol, Stefan Ambs, Curtis C Harris, Michael Graham Espey, Lorne J Hofseth, Michael D Wyatt
Carcinogenesis 2009-12-01
Chronic generation of reactive nitrogen species (RNS) can cause DNA damage and may also directly modify DNA repair proteins. RNS-modified DNA is repaired predominantly by the base excision repair (BER) pathway, which includes the alkyladenine DNA glycosylase (AAG). The AAG active site contains several tyrosines and cysteines tha...Read More
Bjoern Chapuy, Melanie Panse, Ulf Radunski, Raphael Koch, Dirk Wenzel, Nobuya Inagaki, Detlef Haase, Lorenz Truemper, Gerald G Wulf
Haematologica 2009-11-01
Inhibition of BCR-ABL tyrosine kinase activity has evolved as a mainstay of therapy for patients with chronic myeloid leukemia. However, a fraction of leukemic cells persists under targeted therapy and can lead to disease progression on cessation of treatment. We analyzed bone marrow progenitor cells with the side population phe...Read More
Konstantinos Lefkimmiatis, Mary Pat Moyer, Silvana Curci, Aldebaran M Hofer
PLoS ONE 2009-01-01
While intracellular buffers are widely used to study calcium signaling, no such tool exists for the other major second messenger, cyclic AMP (cAMP). Here we describe a genetically encoded buffer for cAMP based on the high-affinity cAMP-binding carboxy-terminus of the regulatory subunit RIbeta of protein kinase A (PKA). Addition ...Read More
Quan-Guang Zhang, Limor Raz, Ruimin Wang, Dong Han, Liesl De Sevilla, Fang Yang, Ratna K Vadlamudi, Darrell W Brann
Journal of Neuroscience 2009-11-04
The goal of this study was to elucidate the mechanisms of 17beta-estradiol (E(2)) antioxidant and neuroprotective actions in stroke. The results reveal a novel extranuclear receptor-mediated antioxidant mechanism for E(2) during stroke, as well as a hypersensitivity of the CA3/CA4 region to ischemic injury after prolonged hypoes...Read More
Gang Wu, Ah-Lim Tsai, Richard J Kulmacz
Biochemistry (Washington) 2009-12-22
Reaction of prostaglandin H synthase (PGHS) isoforms 1 or 2 with peroxide forms a radical at Tyr385 that is required for cyclooxygenase catalysis and another radical at Tyr504, whose function is unknown. Both tyrosyl radicals are transient and rapidly dissipated by reductants, suggesting that cyclooxygenase catalysis might be vu...Read More
Silvia Canudas, Susan Smith
Journal of Cell Biology 2009-10-19
Replicated sister chromatids are held together until mitosis by cohesin, a conserved multisubunit complex comprised of Smc1, Smc3, Scc1, and Scc3, which in vertebrate cells exists as two closely related homologues (SA1 and SA2). Here, we show that cohesin(SA1) and cohesin(SA2) are differentially required for telomere and centrom...Read More
Nickolay Neznanov, Anton Gorbachev, Lubov Neznanova, Andrei Komarov, Katerina Gurova, Alexander Gasparian, Amiya Banerjee, Alexandru Almasan, Robert Fairchild, Andrei Gudkov
Cell Cycle 2009-12-01
The number of physical conditions and chemical agents induce accumulation of misfolded proteins creating proteotoxic stress. This leads to activation of adaptive pro-survival pathway, known as heat shock response (HSR), resulting in expression of additional chaperones. Several cancer treatment approaches, such as proteasome inhi...Read More
K C Walls, Arindam P Ghosh, Mary E Ballestas, Barbara J Klocke, Kevin A Roth
Journal of neuropathology and experimental neurology 2009-12-01
Perinatal hypoxia-ischemia may result in long-term neurological deficits. In addition to producing neuron death, HI causes death of neural precursor cells (NPCs) in the developing brain. To characterize the molecular pathways that regulate hypoxia-induced death of NPCs, we treated a mouse neural stem cell line (C17.2 cells) and ...Read More
Stephan Clavel, Sandrine Siffroi-Fernandez, Anne Sophie Coldefy, Kim Boulukos, Didier F Pisani, Benoît Dérijard
Molecular and Cellular Biology 2010-01-01
Muscle atrophy is a debilitating process associated with many chronic wasting diseases, like cancer, diabetes, sepsis, and renal failure. Rapid loss of muscle mass occurs mainly through the activation of protein breakdown by the ubiquitin proteasome pathway. Foxo3a transcription factor is critical for muscle atrophy, since it ac...Read More
Qunzhou Zhang, Shihong Shi, Yi Liu, Jettie Uyanne, Yufang Shi, Songtao Shi, Anh D Le
Journal of Immunology 2009-12-15
Aside from the well-established self-renewal and multipotent differentiation properties, mesenchymal stem cells exhibit both immunomodulatory and anti-inflammatory roles in several experimental autoimmune and inflammatory diseases. In this study, we isolated a new population of stem cells from human gingiva, a tissue source easi...Read More
C Díaz-Ruiz, J Wang, H Ksiezak-Reding, L Ho, X Qian, N Humala, S Thomas, P Martínez-Martín, G M Pasinetti
Cardiovascular Psychiatry and Neurology 2009-01-01
Epidemiological evidence suggests that hypertension may accelerate the onset and progression of Alzheimer's disease (AD). In this study, we explored the role of hypertension in the neurodegenerative changes associated with Abeta and tau aggregation. We induced hypertension in APP(swe) Tg2576 and P301L-tauTg mouse models. In Tg25...Read More
Hiroko Yano, Risa Torkin, Laura Andrés Martin, Moses V Chao, Kenneth K Teng
Journal of Neuroscience 2009-11-25
Although mature neurotrophins are well described trophic factors that elicit retrograde survival signaling, the precursor forms of neurotrophins (i.e., proneurotrophins) can function as high-affinity apoptotic ligands for selected neural populations. An outstanding question is whether target-derived proneurotrophins might affect...Read More
Dmitriy Krepkiy, Mihaela Mihailescu, J Alfredo Freites, Eric V Schow, David L Worcester, Klaus Gawrisch, Douglas J Tobias, Stephen H White, Kenton J Swartz
Nature 2009-11-26
Despite the growing number of atomic-resolution membrane protein structures, direct structural information about proteins in their native membrane environment is scarce. This problem is particularly relevant in the case of the highly charged S1-S4 voltage-sensing domains responsible for nerve impulses, where interactions with th...Read More
He-Ping Tian, Bao-Sheng Huang, Jie Zhao, Xiao-Han Hu, Jun Guo, Li-Xin Li
BMC Neuroscience 2009-01-01
Neurogenesis in the adult mammalian hippocampus may contribute to repairing the brain after injury. However, Molecular mechanisms that regulate neuronal cell proliferation in the dentate gyrus (DG) following ischemic stroke insult are poorly understood. This study was designed to investigate the potential regulatory capacity of ...Read More
Young Mee Kim, Ji Eun Park, Jin Young Kim, Hee Kyung Lim, Jae Kook Nam, Moonjae Cho, Kyung-Sue Shin
Journal of Korean Medical Science 2009-12-01
Chronic granulomatous disease (CGD) is a rare hereditary disorder characterized by recurrent life-threatening bacterial and fungal infections. The underlying defect in CGD is an inability of phagocytes to produce reactive oxygen species as a result of defects in NADPH oxidase. Considering that CGD generally affects about 3-4 in ...Read More
Thibaut Quillard, Julie Devalliere, Mathias Chatelais, Flora Coulon, Céline Séveno, Mathilde Romagnoli, Sophie Barillé Nion, Béatrice Charreau
PLoS ONE 2009-01-01
Notch signaling pathway controls key functions in vascular and endothelial cells (ECs) where Notch4 plays a major role. However, little is known about the contribution of other Notch receptors. This study investigated regulation of Notch2 and further examined its implication in EC dysfunction. Here, we provide evidence for a nov...Read More
David H Tompkins, Valérie Besnard, Alexander W Lange, Susan E Wert, Angela R Keiser, April N Smith, Richard Lang, Jeffrey A Whitsett
PLoS ONE 2009-01-01
The bronchioles of the murine lung are lined by a simple columnar epithelium composed of ciliated, Clara, and goblet cells that together mediate barrier function, mucociliary clearance and innate host defense, vital for pulmonary homeostasis. In the present work, we demonstrate that expression of Sox2 in Clara cells is required ...Read More
Lukasz Kozera, Ed White, Sarah Calaghan
PLoS ONE 2009-01-01
Many ion channels are preferentially located in caveolae where compartmentalisation/scaffolding with signal transduction components regulates their activity. Channels that are mechanosensitive may be additionally dependent on caveolar control of the mechanical state of the membrane. Here we test which mechanism underlies caveola...Read More
T Leon Lassiter, Ian T Ryde, Edward D Levin, Frederic J Seidler, Theodore A Slotkin
Brain Research Bulletin 2010-01-15
Organophosphates are developmental neurotoxicants but recent evidence also points to metabolic dysfunction. We determined whether neonatal parathion exposure in rats has long-term effects on regulation of adipokines and lipid peroxidation. We also assessed the interaction of these effects with increased fat intake. Rats were giv...Read More
Adelene Y Tan, James L Manley
Molecular and Cellular Biology 2010-01-01
RNA transcription by all the three RNA polymerases (RNAPs) is tightly controlled, and loss of regulation can lead to, for example, cellular transformation and cancer. While most transcription factors act specifically with one polymerase, a small number have been shown to affect more than one polymerase to coordinate overall leve...Read More
Christopher S McAllister, Ann M Toth, Ping Zhang, Patricia Devaux, Roberto Cattaneo, Charles E Samuel
Journal of Virology 2010-01-01
The measles virus P gene products V and C antagonize the host interferon (IFN) response, blocking both IFN signaling and production. Using Moraten vaccine strain-derived measles virus and isogenic mutants deficient for either V or C protein production (V(ko) and C(ko), respectively), we observed that the C(ko) virus was a potent...Read More
Gaëlle Boncompain, Benoît Schneider, Cédric Delevoye, Odile Kellermann, Alice Dautry-Varsat, Agathe Subtil
Infection and Immunity 2010-01-01
Reactive oxygen species (ROS) are many-faceted compounds involved in cell defense against pathogens, as well as in cell signaling. Their involvement in the response to infection in epithelial cells remains poorly documented. Here, we investigated the production of ROS during infection with Chlamydia trachomatis, a strict intrace...Read More
Weizhong Chang, Aileen M Barnes, Wayne A Cabral, Joann N Bodurtha, Joan C Marini
Human Molecular Genetics 2010-01-15
Null mutations in cartilage-associated protein (CRTAP) and prolyl 3-hydroxylase 1 (P3H1/LEPRE1) cause types VII and VIII OI, respectively, two novel recessive forms of osteogenesis imperfecta (OI) with severe to lethal bone dysplasia and overmodification of the type I collagen helical region. CRTAP and P3H1 form a complex with c...Read More
Christiane Albert-Weissenberger, Tobias Sahr, Odile Sismeiro, Jörg Hacker, Klaus Heuner, Carmen Buchrieser
Journal of Bacteriology 2010-01-01
The bacterial pathogen Legionella pneumophila responds to environmental changes by differentiation. At least two forms are well described: replicative bacteria are avirulent; in contrast, transmissive bacteria express virulence traits and flagella. Phenotypic analysis, Western blotting, and electron microscopy of mutants of the ...Read More
John Maciejowski, Kelly A George, Marie-Emilie Terret, Chao Zhang, Kevan M Shokat, Prasad V Jallepalli
Journal of Cell Biology 2010-07-12
The spindle assembly checkpoint (SAC) in mammals uses cytosolic and kinetochore-based signaling pathways to inhibit anaphase. In this study, we use chemical genetics to show that the protein kinase Mps1 regulates both aspects of the SAC. Human MPS1-null cells were generated via gene targeting and reconstituted with either the wi...Read More
Laura Tedesco, Alessandra Valerio, Marta Dossena, Annalisa Cardile, Maurizio Ragni, Claudio Pagano, Uberto Pagotto, Michele O Carruba, Roberto Vettor, Enzo Nisoli
Diabetes 2010-11-01
Cannabinoid type 1 (CB1) receptor is involved in whole-body and cellular energy metabolism. We asked whether CB1 receptor stimulation was able to decrease mitochondrial biogenesis in different metabolically active tissues of obese high-fat diet (HFD)-fed mice. The effects of selective CB1 agonist arachidonyl-2-chloroethanolamide...Read More
Samriddha Ray, Kazunori Kume, Sneha Gupta, Wanzhong Ge, Mohan Balasubramanian, Dai Hirata, Dannel McCollum
Journal of Cell Biology 2010-09-06
The mechanisms that regulate cytoskeletal remodeling during the transition between mitosis and interphase are poorly understood. In fission yeast the MOR pathway promotes actin polarization to cell tips in interphase, whereas the SIN signaling pathway drives actomyosin ring assembly and cytokinesis. We show that the SIN inhibits...Read More
Sharon A Williams, Yuka Harata-Lee, Iain Comerford, Robin L Anderson, Mark J Smyth, Shaun R McColl
Molecular Cancer 2010-01-01
A growing body of work implicates chemokines, in particular CXCL12 and its receptors, in the progression and site-specific metastasis of various cancers, including breast cancer. Various agents have been used to block the CXCL12-CXCR4 interaction as a means of inhibiting cancer metastasis. However, as a potent chemotactic factor...Read More
Amparo C Villablanca, Kent E Pinkerton, John C Rutledge
Journal of Cardiovascular Translational Research 2010-12-01
Maternal mainstream tobacco smoking is known to have adverse outcomes on fetal respiratory function; however, no data is currently available on the effects of passive exposure to tobacco smoking and environmental tobacco smoke (ETS) on fetal systemic arterial structure and function. Eight pregnant rhesus macaque monkeys were stu...Read More
Zhen Mao, Yuan-Lin Zheng, Yan-Qiu Zhang
International Journal of Molecular Sciences 2010-01-01
Nonylphenol (NP) is a degradation product of nonylphenol polyethoxylates, which are widely used in the production of industrial and consumer surfactants. The aim of the present study was to evaluate the effect of NP on the antioxidant capacity and cognitive ability of mice. NP was given orally by gavages at doses of 0, 50, 100, ...Read More
Sandra Muschiol, Gaelle Boncompain, François Vromman, Pierre Dehoux, Staffan Normark, Birgitta Henriques-Normark, Agathe Subtil
Infection and Immunity 2011-02-01
Chlamydiae are Gram-negative, obligate intracellular pathogens that replicate within a membrane-bounded compartment termed an inclusion. Throughout their development, they actively modify the eukaryotic environment. The type III secretion (TTS) system is the main process by which the bacteria translocate effector proteins into t...Read More
Marco Lalle, Chiara Currà, Fabio Ciccarone, Tomasino Pace, Serena Cecchetti, Luca Fantozzi, Bernhard Ay, Catherine Braun Breton, Marta Ponzi
Journal of Biological Chemistry 2011-01-14
The malaria parasite invades the terminally differentiated erythrocytes, where it grows and multiplies surrounded by a parasitophorous vacuole. Plasmodium blood stages translocate newly synthesized proteins outside the parasitophorous vacuole and direct them to various erythrocyte compartments, including the cytoskeleton and the...Read More
Ryan P Hobbs, Evangeline V Amargo, Agila Somasundaram, Cory L Simpson, Murali Prakriya, Mitchell F Denning, Kathleen J Green
FASEB Journal 2011-03-01
Darier's disease (DD) is an inherited autosomal-dominant skin disorder characterized histologically by loss of adhesion between keratinocytes. DD is typically caused by mutations in sarcoendoplasmic reticulum Ca(2+)-ATPase isoform 2 (SERCA2), a major regulator of intracellular Ca(2+) homeostasis in the skin. However, a defined r...Read More
Kazuchika Nishitsuji, Takashi Hosono, Kenji Uchimura, Makoto Michikawa
Journal of Biological Chemistry 2011-02-25
Lipoprotein lipase (LPL) is a member of a lipase family known to hydrolyze triglyceride molecules in plasma lipoprotein particles. LPL also plays a role in the binding of lipoprotein particles to cell-surface molecules, including sulfated glycosaminoglycans (GAGs). LPL is predominantly expressed in adipose and muscle but is also...Read More
Beatriz Russell, Saumitri Bhattacharyya, Jeremy Keirsey, April Sandy, Patrick Grierson, Erin Perchiniak, Juraj Kavecansky, Samir Acharya, Joanna Groden
Cancer Research 2011-01-15
Cells deficient in the recQ-like helicase BLM are characterized by chromosome changes that suggest the disruption of normal mechanisms needed to resolve recombination intermediates and to maintain chromosome stability. Human BLM and topoisomerase IIa interact directly via amino acids 489-587 of BLM and colocalize predominantly i...Read More
Stefania Bottardi, Farah A Zmiri, Vincent Bourgoin, Julie Ross, Lionel Mavoungou, Eric Milot
Nucleic Acids Research 2011-05-01
Ikaros is associated with both gene transcriptional activation and repression in lymphocytes. Ikaros acts also as repressor of human ?-globin (hu?-) gene transcription in fetal and adult erythroid cells. Whether and eventually, how Ikaros can function as a transcriptional activator in erythroid cells remains poorly understood. R...Read More
Assaf Shapira, Meital Gal-Tanamy, Limor Nahary, Dana Litvak-Greenfeld, Romy Zemel, Ran Tur-Kaspa, Itai Benhar
PLoS ONE 2011-01-01
The synthesis of inactive enzyme precursors, also known as "zymogens," serves as a mechanism for regulating the execution of selected catalytic activities in a desirable time and/or site. Zymogens are usually activated by proteolytic cleavage. Many viruses encode proteases that execute key proteolytic steps of the viral life cyc...Read More
Raffaella Nativio, Angela Sparago, Yoko Ito, Rosanna Weksberg, Andrea Riccio, Adele Murrell
Human Molecular Genetics 2011-04-01
Hyper- and hypomethylation at the IGF2-H19 imprinting control region (ICR) result in reciprocal changes in IGF2-H19 expression and the two contrasting growth disorders, Beckwith-Wiedemann syndrome (BWS) and Silver-Russell syndrome (SRS). DNA methylation of the ICR controls the reciprocal imprinting of IGF2 and H19 by preventing ...Read More
Lynette Steele, Fiona Errington, Robin Prestwich, Elizabeth Ilett, Kevin Harrington, Hardev Pandha, Matt Coffey, Peter Selby, Richard Vile, Alan Melcher
Molecular Cancer 2011-01-01
As well as inducing direct oncolysis, reovirus treatment of melanoma is associated with activation of innate and adaptive anti-tumour immune responses. Here we characterise the effects of conditioned media from reovirus-infected, dying human melanoma cells (reoTCM), in the absence of live virus, to address the immune bystander p...Read More
Fábio S Lira, José C Rosa, Claudio A Cunha, Eliane B Ribeiro, Claudia Oller do Nascimento, Lila M Oyama, João F Mota
Lipids in Health and Disease 2011-01-01
It is well known that high fat diets (HFDs) induce obesity and an increase in proinflammatory adipokines. Interleukin-6 (IL-6) is considered the major inflammatory mediator in obesity. Obesity is associated with a vitamin deficiency, especially of vitamins E and D3. We examined the effects of vitamin D3 and vitamin E supplementa...Read More
Ana Gómez-Ruiz, Fermín I Milagro, Javier Campión, J Alfredo Martínez, Carlos de Miguel
Lipids in Health and Disease 2011-01-01
The effect of food intake on caveolin expression in relation to insulin signalling was studied in skeletal muscle and adipocytes from retroperitoneal (RP) and subcutaneous (SC) adipose tissue, comparing fasted (F) to not fasted (NF) rats that had been fed a control or high-fat (HF) diet for 72 days. Serum glucose was analysed en...Read More
Sunday S Oladipupo, Song Hu, Andrea C Santeford, Junjie Yao, Joanna R Kovalski, Ralph V Shohet, Konstantin Maslov, Lihong V Wang, Jeffrey M Arbeit
Blood 2011-04-14
Neovascularization is a crucial component of tumor growth and ischemia. Although prior work primarily used disease models, delineation of neovascularization in the absence of disease can reveal intrinsic mechanisms of microvessel regulation amenable to manipulation in illness. We created a conditional model of epithelial HIF-1 i...Read More
Horng-Ru Lin, Don Ganem
PNAS 2011-03-29
It is widely believed that functional mammalian microRNA (miRNA) recognition sequences are located preferentially in the 3' untranslated region (3'UTR) of target mRNAs. Nonetheless, putative miRNA target sites within coding regions have been found at lower frequency in genome-wide studies, and several have been genetically valid...Read More
Elke E Noens, Chris Williams, Madhankumar Anandhakrishnan, Christian Poulsen, Matthias T Ehebauer, Matthias Wilmanns
BMC Biotechnology 2011-01-01
The non-pathogenic bacterium Mycobacterium smegmatis is widely used as a near-native expression host for the purification of Mycobacterium tuberculosis proteins. Unfortunately, the Hsp60 chaperone GroEL1, which is relatively highly expressed, is often co-purified with polyhistidine-tagged recombinant proteins as a major contamin...Read More
Junko Tanaka, Hiroshi Yanagawa, Nobuhide Doi
PLoS ONE 2011-01-01
Although modern proteins consist of 20 different amino acids, it has been proposed that primordial proteins consisted of a small set of amino acids, and additional amino acids have gradually been recruited into the genetic code. This hypothesis has recently been supported by comparative genome sequence analysis, but no direct ex...Read More
Xiaonan Du, Bobbi Fleiss, Hongfu Li, Barbara D'angelo, Yanyan Sun, Changlian Zhu, Henrik Hagberg, Ofer Levy, Carina Mallard, Xiaoyang Wang
PLoS ONE 2011-01-01
Inflammation is associated with perinatal brain injury but the underlying mechanisms are not completely characterized. Stimulation of Toll-like receptors (TLRs) through specific agonists induces inflammatory responses that trigger both innate and adaptive immune responses. The impact of engagement of TLR2 signaling pathways on t...Read More
Renato Millioni, Serena Tolin, Lucia Puricelli, Stefano Sbrignadello, Gian Paolo Fadini, Paolo Tessari, Giorgio Arrigoni
PLoS ONE 2011-01-01
To date, the complexity of the plasma proteome exceeds the analytical capacity of conventional approaches to isolate lower abundance proteins that may prove to be informative biomarkers. Only complex multistep separation strategies have been able to detect a substantial number of low abundance proteins (<100 ng/ml). The first st...Read More
Eitan Israeli, Eugenia Yakunin, Yonaton Zarbiv, Amir Hacohen-Solovich, Haya Kisos, Virginie Loeb, Michal Lichtenstein, Tziona Ben-Gedalya, Ofra Sabag, Eli Pikarsky, Haya Lorberboum-Galski, Ronit Sharon
PLoS ONE 2011-01-01
Alpha Synuclein (a-Syn) is a protein implicated in mechanisms of neuronal degeneration in Parkinson's disease (PD). a-Syn is primarily a neuronal protein, however, its expression is found in various tumors including ovarian, colorectal and melanoma tumors. It has been hypothesized that neurodegeneration may share common mechanis...Read More
Xiaoyin Shan, Kenneth B Margulies
PLoS ONE 2011-01-01
Though long known to affect smooth muscle biology, recent studies indicate that phosphodiesterase 5 (PDE5) is also expressed in myocardium. Recognizing that the regulation of PDE5 in hypertrophy is not well understood, we assessed the response of PDE5 expression and the level of cGMP-dependent kinase I (cGKI) in the left and rig...Read More
John H Lillvis, Robert Erdman, Charles M Schworer, Alicia Golden, Kimberly Derr, Zoran Gatalica, Laura A Cox, Jianbin Shen, Richard S Vander Heide, Guy M Lenk, Leigh Hlavaty, Li Li, James R Elmore, David P Franklin, John L Gray, Robert P Garvin, David J Carey, Wayne D Lancaster, Gerard Tromp, Helena Kuivaniemi
BMC Physiology 2011-01-01
The infrarenal abdominal aorta exhibits increased disease susceptibility relative to other aortic regions. Allograft studies exchanging thoracic and abdominal segments showed that regional susceptibility is maintained regardless of location, suggesting substantial roles for embryological origin, tissue composition and site-speci...Read More
Beatrice Arosio, Luigina Mastronardi, Cristina Gussago, Paola Nicolini, Alessandra Casè, Eleonora Ziglioli, Luigi Bergamaschini
International Journal of Alzheimer's Disease 2011-01-01
Adenosine suppresses immune responses through the A(2A) receptor (A(2A)R). This study investigated the interleukin 10 (IL-10) genetic profile and the expression of A(2A)R in peripheral blood mononuclear cells (PBMCs) of patients with mild cognitive impairment (MCI), Alzheimer disease (AD), and age-matched controls to verify, if ...Read More
Nazif Alic, T Daniel Andrews, Maria E Giannakou, Irene Papatheodorou, Cathy Slack, Matthew P Hoddinott, Helena M Cochemé, Eugene F Schuster, Janet M Thornton, Linda Partridge
Molecular Systems Biology 2011-01-01
FoxO transcription factors, inhibited by insulin/insulin-like growth factor signalling (IIS), are crucial players in numerous organismal processes including lifespan. Using genomic tools, we uncover over 700 direct dFOXO targets in adult female Drosophila. dFOXO is directly required for transcription of several IIS components an...Read More
Alexandra Zinoviev, Mélissa Léger, Gerhard Wagner, Michal Shapira
Nucleic Acids Research 2011-10-01
In eukaryotes, exposure to stress conditions causes a shift from cap-dependent to cap-independent translation. In trypanosomatids, environmental switches are the driving force of a developmental program of gene expression, but it is yet unclear how their translation machinery copes with their constantly changing environment. Try...Read More
Monika Sztretye, Jianxun Yi, Lourdes Figueroa, Jingsong Zhou, Leandro Royer, Eduardo Ríos
Journal of General Physiology 2011-08-01
Current fluorescent monitors of free [Ca(2+)] in the sarcoplasmic reticulum (SR) of skeletal muscle cells are of limited quantitative value. They provide either a nonratio signal that is difficult to calibrate and is not specific or, in the case of Forster resonant energy transfer (FRET) biosensors, a signal of small dynamic ran...Read More
David Du Pasquier, Aude Dupré, Catherine Jessus
PLoS ONE 2011-01-01
Ovulated eggs possess maternal apoptotic execution machinery that is inhibited for a limited time. The fertilized eggs switch off this time bomb whereas aged unfertilized eggs and parthenogenetically activated eggs fail to stop the timer and die. To investigate the nature of the molecular clock that triggers the egg decision of ...Read More
Dekel D Bar-Lev, Nurit Degani-Katzav, Alexander Perelman, Yoav Paas
Journal of Biological Chemistry 2011-12-23
Cys-loop receptors are pentameric ligand-gated ion channels (pLGICs) that bind neurotransmitters to open an intrinsic transmembrane ion channel pore. The recent crystal structure of a prokaryotic pLGIC from the cyanobacterium Gloeobacter violaceus (GLIC) revealed that it naturally lacks an N-terminal extracellular a helix and an...Read More
Tyler R Schleicher, Spencer V Nyholm
PLoS ONE 2011-01-01
The beneficial symbiosis between the Hawaiian bobtail squid, Euprymna scolopes, and the bioluminescent bacterium, Vibrio fischeri, provides a unique opportunity to study host/microbe interactions within a natural microenvironment. Colonization of the squid light organ by V. fischeri begins a lifelong association with a regulated...Read More
Andrew R S T Yang, Juan Liu, Heon S Yi, Kaitlin T Warnock, Mingfei Wang, Harry L June, Adam C Puche, Ahmed Elnabawi, Werner Sieghart, Laure Aurelian, Harry L June
Frontiers in Neuroscience 2011-01-01
Binge drinking, frequently referred to clinically as problem or hazardous drinking, is a pattern of excessive alcohol intake characterized by blood alcohol levels =0.08?g% within a 2-h period. Here, we show that overexpression of a1 subunits of the GABA(A) receptor contributes to binge drinking, and further document that this in...Read More
F R Lamont, D C Tomlinson, P A Cooper, S D Shnyder, J D Chester, M A Knowles
British Journal of Cancer 2011-01-04
Activating mutations of FGFR3 are frequently identified in superficial urothelial carcinoma (UC) and increased expression of FGFR1 and FGFR3 are common in both superficial and invasive UC. The effects of inhibition of receptor activity by three small molecule inhibitors (PD173074, TKI-258 and SU5402) were investigated in a panel...Read More
Mounira Chaki, Raquel Valderrama, Ana M Fernández-Ocaña, Alfonso Carreras, Maria V Gómez-Rodríguez, José R Pedrajas, Juan C Begara-Morales, Beatriz Sánchez-Calvo, Francisco Luque, Marina Leterrier, Francisco J Corpas, Juan B Barroso
Journal of Experimental Botany 2011-03-01
Nitric oxide (NO) and related molecules such as peroxynitrite, S-nitrosoglutathione (GSNO), and nitrotyrosine, among others, are involved in physiological processes as well in the mechanisms of response to stress conditions. In sunflower seedlings exposed to five different adverse environmental conditions (low temperature, mecha...Read More
Yuhai Zhao, Frederic Calon, Carl Julien, Jeremy W Winkler, Nicos A Petasis, Walter J Lukiw, Nicolas G Bazan
PLoS ONE 2011-01-01
Neuroprotectin D1 (NPD1) is a stereoselective mediator derived from the omega-3 essential fatty acid docosahexaenoic acid (DHA) with potent inflammatory resolving and neuroprotective bioactivity. NPD1 reduces Aß42 peptide release from aging human brain cells and is severely depleted in Alzheimer's disease (AD) brain. Here we fur...Read More
Roya Babaei-Jadidi, Ningning Li, Anas Saadeddin, Bradley Spencer-Dene, Anett Jandke, Belal Muhammad, ElSayed E Ibrahim, Ranjithmenon Muraleedharan, Mohammed Abuzinadah, Hayley Davis, Annabelle Lewis, Susan Watson, Axel Behrens, Ian Tomlinson, Abdolrahman Shams Nateri
Journal of Experimental Medicine 2011-02-14
The Fbxw7 (F-box/WD repeat-containing protein 7; also called CDC4, Sel10, Ago, and Fbw7) component of the SCF (Skp1/Cullin/F-box protein) E3 ubiquitin ligase complex acts as a tumor suppressor in several tissues and targets multiple transcriptional activators and protooncogenes for ubiquitin-mediated degradation. To understand F...Read More
Huifang Ma, Jiaming Zhang, Irwin B Levitan
Journal of General Physiology 2011-02-01
Modulation of ion channels by regulatory proteins within the same macromolecular complex is a well-accepted concept, but the physiological consequences of such modulation are not fully understood. Slowpoke (Slo), a potassium channel critical for action potential repolarization and transmitter release, is regulated by Slo channel...Read More
Adele Hannigan, Asif M Qureshi, Colin Nixon, Penelope M Tsimbouri, Sarah Jones, Adrian W Philbey, Joanna B Wilson
Molecular Cancer 2011-01-01
The importance of the malignant cell environment to its growth and survival is becoming increasingly apparent, with dynamic cross talk between the neoplastic cell, the leukocyte infiltrate and the stroma. Most cancers are accompanied by leukocyte infiltration which, contrary to an anticipated immuno-protective role, could be con...Read More
Huan Long, Hans H Bock, Ting Lei, Xuejun Chai, Jihong Yuan, Joachim Herz, Michael Frotscher, Zaiqing Yang
BMC Neuroscience 2011-01-01
Disabled-1 (Dab1) is an adaptor protein that is essential for the intracellular transduction of Reelin signaling, which regulates the migration and differentiation of postmitotic neurons during brain development in vertebrates. Dab1 function depends on its tyrosine phosphorylation by Src family kinases, especially Fyn. We have i...Read More
Christian Leo, Valentina Sala, Mara Morello, Amedeo Chiribiri, Ilan Riess, Daniele Mancardi, Stefano Schiaffino, Carola Ponzetto, Tiziana Crepaldi
PLoS ONE 2011-01-01
The Hepatocyte Growth Factor (HGF) is a pleiotropic cytokine involved in many physiological processes, including skeletal muscle, placenta and liver development. Little is known about its role and that of Met tyrosine kinase receptor in cardiac development. In this study, we generated two transgenic mice with cardiac-specific, t...Read More
N Heidari, M A Hicks, H Harada
Cell Death and Disease 2010-01-01
Glucocorticoids (GCs) are common components of many chemotherapeutic regimens for lymphoid malignancies including acute lymphoblastic leukemia (ALL). The BCL-2 family has an essential role in regulating GC-induced cell death. Here we show that downregulation of antiapoptotic BCL-2 family proteins, especially MCL-1, enhances GC-i...Read More
Stuart T Perry, Michael D Buck, Steven M Lada, Christian Schindler, Sujan Shresta
PLoS Pathogens 2011-02-01
Dengue virus (DENV) is a mosquito-borne flavivirus, and symptoms of infection range from asymptomatic to the severe dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS). High viral loads correlate with disease severity, and both type I & II interferons (IFNs) are crucial for controlling viral replication. We have previously ...Read More
Heinz Fischer, Jennifer Scherz, Sandra Szabo, Michael Mildner, Charaf Benarafa, Alicia Torriglia, Erwin Tschachler, Leopold Eckhart
PLoS ONE 2011-01-01
The cornified layer, the stratum corneum, of the epidermis is an efficient barrier to the passage of genetic material, i.e. nucleic acids. It contains enzymes that degrade RNA and DNA which originate from either the living part of the epidermis or from infectious agents of the environment. However, the molecular identities of th...Read More
Andrew M Edwards, Ursula Potter, Nicola A G Meenan, Jennifer R Potts, Ruth C Massey
PLoS ONE 2011-01-01
Staphylococcus aureus is a commensal organism and a frequent cause of skin and soft tissue infections, which can progress to serious invasive disease. This bacterium uses its fibronectin binding proteins (FnBPs) to invade host cells and it has been hypothesised that this provides a protected niche from host antimicrobial defence...Read More
Youngjoo Kwon, Edna Cukierman, Andrew K Godwin
PLoS ONE 2011-01-01
Epithelial ovarian cancer is an aggressive and deadly disease and understanding its invasion mechanisms is critical for its treatment. We sought to study the penetration/invasion of ovarian tumor cells into extracellular matrices (ECMs) using a fibroblast-derived three-dimensional (3D) culture model and time-lapse and confocal i...Read More
Elma Aflaki, Nariman A B Balenga, Petra Luschnig-Schratl, Heimo Wolinski, Silvia Povoden, Prakash G Chandak, Juliane G Bogner-Strauss, Sandra Eder, Viktoria Konya, Sepp-Dieter Kohlwein, Akos Heinemann, Dagmar Kratky
Cellular and Molecular Life Sciences 2011-12-01
Infiltration of monocytes and macrophages into the site of inflammation is critical in the progression of inflammatory diseases such as atherosclerosis. Cell migration is dependent on the continuous organization of the actin cytoskeleton, which is regulated by members of the small Rho GTPase family (RhoA, Cdc42, Rac) that are al...Read More
Yanhua Zhao, Srijita Banerjee, Nilay Dey, Wanda S LeJeune, Partha S Sarkar, Reynolds Brobey, Kevin P Rosenblatt, Ronald G Tilton, Sanjeev Choudhary
Diabetes 2011-07-01
Klotho is an antiaging hormone present in the kidney that extends the lifespan, regulates kidney function, and modulates cellular responses to oxidative stress. We investigated whether Klotho levels and signaling modulate inflammation in diabetic kidneys. Renal Klotho expression was determined by quantitative real-time PCR and i...Read More
Christian Mühlfeld, Suman Kumar Das, Frank R Heinzel, Albrecht Schmidt, Heiner Post, Silvia Schauer, Tamara Papadakis, Wolfgang Kummer, Gerald Hoefler
PLoS ONE 2011-01-01
Cancer is often associated with cachexia, cardiovascular symptoms and autonomic dysregulation. We tested whether extracardiac cancer directly affects the innervation of left ventricular myocardium. Mice injected with Lewis lung carcinoma cells (tumor group, TG) or PBS (control group, CG) were analyzed after 21 days. Cardiac func...Read More
Bader E Hussaini, Xiu-Gui Lu, J Alan Wolfe, Hemant S Thatte
Journal of Cardiothoracic Surgery 2011-01-01
Endothelial injury during harvest influences graft patency post CABG. We have previously shown that endoscopic harvest causes structural and functional damage to the saphenous vein (SV) endothelium. However, causes of such injury may depend on the extraction technique. In order to assess this supposition, we evaluated the effect...Read More
Mikhail Lomonosov, El Kahina Meziane, Hongtao Ye, David E Nelson, Suzanne J Randle, Heike Laman
PLoS ONE 2011-01-01
Fbxo7 is an unusual F box protein that augments D-type cyclin complex formation with Cdk6, but not Cdk4 or Cdk2, and its over-expression has been demonstrated to transform immortalised fibroblasts in a Cdk6-dependent manner. Here we present new evidence in vitro and in vivo on the oncogenic potential of this regulatory protein i...Read More
Ying Liu, Xiao-Qin Chen, Heng-Xing Liang, Feng-Xiang Zhang, Bo Zhang, Jie Jin, Yong-Long Chen, Yong-Xian Cheng, Guang-Biao Zhou
PLoS ONE 2011-01-01
Multiple myeloma (MM) is a disease of cell cycle dysregulation while cell cycle modulation can be a target for MM therapy. In this study we investigated the effects and mechanisms of action of a sesquiterpene lactone 6-O-angeloylplenolin (6-OAP) on MM cells. MM cells were exposed to 6-OAP and cell cycle distribution were analyze...Read More
Boyko S Atanassov, Sharon Y R Dent
EMBO Reports 2011-09-01
Ubiquitin-specific protease 22 (USP22) edits the histone code by deubiquitinating H2A and H2B as part of the mammalian SAGA (Spt-Ada-Gcn5) complex, and is required for transcriptional regulation and normal cell-cycle progression. Here, we show that USP22 affects the expression of p21 by altering far upstream element (FUSE)-bindi...Read More
Marcelo L Lamers, Maíra E S Almeida, Miguel Vicente-Manzanares, Alan F Horwitz, Marinilce F Santos
PLoS ONE 2011-01-01
Deficient wound healing in diabetic patients is very frequent, but the cellular and molecular causes are poorly defined. In this study, we evaluate the hypothesis that high glucose concentrations inhibit cell migration. Using CHO.K1 cells, NIH-3T3 fibroblasts, mouse embryonic fibroblasts and primary skin fibroblasts from control...Read More
Emily Turner-Brannen, Ka-Yee Choi, Dustin N D Lippert, John P Cortens, Robert E W Hancock, Hani El-Gabalawy, Neeloffer Mookherjee
Arthritis research & therapy 2011-01-01
Innate defence regulator (IDR) peptides are synthetic cationic peptides, variants of naturally occurring innate immune effector molecules known as host defence peptides. IDR peptides were recently demonstrated to limit infection-associated inflammation selectively without compromising host innate immune functions. This study exa...Read More
Huili Li, Brenda R Baldwin, Cynthia A Zahnow
Molecular Cancer 2011-01-01
The transcription factor, CCAAT enhancer binding protein-ß (C/EBPß), is expressed as several distinct protein isoforms (LAP1, LAP2 and LIP) that have opposing actions in cellular proliferation and differentiation. Increases in the ratio of LIP/LAP are associated with aggressive, metastatic breast cancer; however, little is known...Read More
Vijayababu M Radhakrishnan, Charles W Putnam, Wenqing Qi, Jesse D Martinez
BMC Cancer 2011-01-01
14-3-3 proteins are a family of highly conserved proteins that are involved in a wide range of cellular processes. Recent evidence indicates that some of these proteins have oncogenic activity and that they may promote tumorigenesis. We previously showed that one of the 14-3-3 family members, 14-3-3 gamma, is over expressed in h...Read More
Rakhi Srivastava, Daman Saluja, Bilikere S Dwarakanath, Madhu Chopra
Evidence-based Complementary and Alternative Medicine 2011-01-01
In Indian traditional medicine, Boerhaavia diffusa (punarnava) roots have been widely used for the treatment of dyspepsia, jaundice, enlargement of spleen, abdominal pain and as an anti-stress agent. Pharmacological evaluation of the crude ethanolic extract of B. diffusa roots has been shown to possess antiproliferative and immu...Read More
Xu Xu, Sounak Gupta, Wenli Hu, Barbara C McGrath, Douglas R Cavener
PLoS ONE 2011-01-01
The ER chaperone GRP78/BiP is a homolog of the Hsp70 family of heat shock proteins, yet GRP78/BiP is not induced by heat shock but instead by ER stress. However, previous studies had not considered more physiologically relevant temperature elevation associated with febrile hyperthermia. In this report we examine the response of ...Read More
Anne Kristin Aksaas, Anja Cv Larsen, Marie Rogne, Ken Rosendal, Anne-Katrine Kvissel, Bjørn Steen Skålhegg
Journal of Molecular Signaling 2011-01-01
Post-transcriptional processing of pre-mRNA takes place in several steps and requires involvement of a number of RNA-binding proteins. How pre-mRNA processing is regulated is in large enigmatic. The catalytic (C) subunit of protein kinase A (PKA) is a serine/threonine kinase, which regulates numerous cellular processes including...Read More
Amr E El Hakim, Yasser E Shahein, Sobhy Abdel-Shafy, Amira Mk Abouelella, Ragaa R Hamed
Journal of Veterinary Science 2011-09-01
In order to identify antigens that can help prevent camel tick infestations, three major glycoproteins (GLPs) about 97, 66 and 40 kDa in size were purified from adult and larval Egyptian ticks, Hyalomma (H.) dromedarii, using a single-step purification method with Con-A sepharose. The purified GLPs were evaluated as vaccines aga...Read More
Michelle B Visser, Adeline Koh, Michael Glogauer, Richard P Ellen
PLoS ONE 2011-01-01
The major outer sheath protein (Msp) of Treponema denticola perturbs actin dynamics in fibroblasts by inducing actin reorganization, including subcortical actin filament assembly, leading to defective calcium flux, diminished integrin engagement of collagen, and retarded cell migration. Yet, its mechanisms of action are unknown....Read More
Dariusz J Smolinski, Bogdan Wróbel, Anna Noble, Agnieszka Zienkiewicz, Alicja Górska-Brylass
Histochemistry and Cell Biology 2011-11-01
Small nuclear ribonucleoproteins (snRNPs) play a fundamental role in pre-mRNA processing in the nucleus. The biogenesis of snRNPs involves a sequence of events that occurs in both the nucleus and cytoplasm. Despite the wealth of biochemical information about the cytoplasmic assembly of snRNPs, little is known about the spatial o...Read More
Roman Paduch, Martyna Kandefer-Szerszen
Cancer Microenvironment 2011-08-01
Cyclooxygenases (COX), prostaglandin E(2) (PGE(2)) and nitric oxide (NO) are believed to be some of the most important factors related to colon cancer growth and metastasis. In this study, we aimed to investigate the associations between COX-2, PGE(2) and NO in co-cultures of human colon cancer spheroids obtained from different ...Read More
Youjun Chen, Xu Tian, Woo-Yang Kim, William D Snider
PLoS ONE 2011-01-01
Conditional deletion of APC leads to marked disruption of cortical development and to excessive axonal branching of cortical neurons. However, little is known about the cell biological basis of this neuronal morphological regulation. Here we show that APC deficient cortical neuronal growth cones exhibit marked disruption of both...Read More
Xiang Lin, Yongyou Zhang, Leyuan Liu, Wallace L McKeehan, Yuemao Shen, Siyang Song, Fen Wang
International Journal of Biological Sciences 2011-01-01
Although the fibroblast growth factor (FGF) signaling axis plays important roles in cell survival, proliferation, and differentiation, the molecular mechanism underlying how the FGF elicits these diverse regulatory signals is not well understood. By using the Frs2a null mouse embryonic fibroblast (MEF) in conjunction with inhibi...Read More
Luyu Yao, Xiaobo Yan, Huijia Dong, David R Nelson, Chen Liu, Xiaoyu Li
Virology Journal 2011-01-01
Interferon Regulatory Factor-3 (IRF-3) plays a central role in the induction of interferon (IFN) production and succeeding interferon-stimulated genes (ISG) expression en route for restraining hepatitis C virus (HCV) infection. Here, we established a stable Huh7.5-IRF3ER cell line expressing a fusion protein of IRF-3 and mouse e...Read More
Akira Nukazuka, Shusaku Tamaki, Kunihiro Matsumoto, Yoichi Oda, Hajime Fujisawa, Shin Takagi
Nature Communications 2011-01-01
The target of rapamycin (TOR), a central regulator for cell growth and metabolism, resides in the two functionally distinct complexes TORC1 and TORC2, which are defined by their adaptors Raptor and Rictor, respectively. How the formation of the two TORCs is orchestrated remains unclear. Here we show the control of TOR partnering...Read More
Amanda J O'Neill, Maria Prencipe, Catherine Dowling, Yue Fan, Laoighse Mulrane, William M Gallagher, Darran O'Connor, Robert O'Connor, Aoife Devery, Claire Corcoran, Sweta Rani, Lorraine O'Driscoll, John M Fitzpatrick, R William G Watson
Molecular Cancer 2011-01-01
There is no effective treatment strategy for advanced castration-resistant prostate cancer. Although Docetaxel (Taxotere®) represents the most active chemotherapeutic agent it only gives a modest survival advantage with most patients eventually progressing because of inherent or acquired drug resistance. The aims of this study w...Read More
Enrico Conte, Mary Fruciano, Evelina Fagone, Elisa Gili, Filippo Caraci, Maria Iemmolo, Nunzio Crimi, Carlo Vancheri
PLoS ONE 2011-01-01
Idiopathic pulmonary fibrosis (IPF) is a progressive fibroproliferative disease characterized by an accumulation of fibroblasts and myofibroblasts in the alveolar wall. Even though the pathogenesis of this fatal disorder remains unclear, transforming growth factor-ß (TGF-ß)-induced differentiation and proliferation of myofibrobl...Read More
Xinbo Zhang, Siyu Crystal Zhang, Dejun Sun, Jiang Hu, Anil Wali, Harvey Pass, Felix Fernandez-Madrid, Michael R Harbut, Naimei Tang
PLoS ONE 2011-01-01
Bisbenzimides, or Hoechst 33258 (H258), and its derivative Hoechst 33342 (H342) are archetypal molecules for designing minor groove binders, and widely used as tools for staining DNA and analyzing side population cells. They are supravital DNA minor groove binders with AT selectivity. H342 and H258 share similar biological effec...Read More
Hari Raghu, Christopher S Gondi, Dzung H Dinh, Meena Gujrati, Jasti S Rao
Molecular Cancer 2011-01-01
uPA/uPAR is a multifunctional system that is over expressed in many cancers and plays a critical role in glioblastoma (GBM) invasion. Previous studies from our lab have also shown that uPA/uPAR down regulation inhibits cancer cell invasion in SNB 19 GBM cells. As Notch 1 is known to be over expressed and promotes invasion in gli...Read More
Young Woo Eom, Jong Eun Lee, Mal Sook Yang, In Keun Jang, Hyo Eun Kim, Doo Hoon Lee, Young Jin Kim, Won Jin Park, Jee Hyun Kong, Kwang Yong Shim, Jong In Lee, Hyun Soo Kim
Yonsei Medical Journal 2011-11-01
This study examined a rapid isolation method decreasing the time and cost of the clinical application of adipose tissue-derived stem cells (ASCs). Aliquots (10 g) of the lipoaspirates were stored at 4°C without supplying oxygen or nutrients. At the indicated time points, the yield of mononuclear cells was evaluated and the stem ...Read More
Jeffery D Eskew, Takrima Sadikot, Pedro Morales, Alicia Duren, Irene Dunwiddie, Megan Swink, Xiaoying Zhang, Stacey Hembruff, Alison Donnelly, Roger A Rajewski, Brian S J Blagg, Jacob R Manjarrez, Robert L Matts, Jeffrey M Holzbeierlein, George A Vielhauer
BMC Cancer 2011-01-01
The molecular chaperone, heat shock protein 90 (Hsp90) has been shown to be overexpressed in a number of cancers, including prostate cancer, making it an important target for drug discovery. Unfortunately, results with N-terminal inhibitors from initial clinical trials have been disappointing, as toxicity and resistance resultin...Read More
Mike Spillane, Neil Schwarz, Sarah Leddy, Tracie Correa, Melodie Minter, Victoria Longoria, Darryn S Willoughby
Nutrition & Metabolism 2011-01-01
The effects of 28 days of heavy resistance training while ingesting the pre- and post-workout supplements, NO-Shotgun® and NO-Synthesize® were determined on body composition, muscle strength and mass, markers of protein synthesis, and clinical safety markers. Nineteen non-resistance-trained males participated in a resistance tra...Read More
Andrea L H Arnett, Dilip Garikipati, Zejing Wang, Stephen Tapscott, Jeffrey S Chamberlain
Frontiers in Microbiology 2011-01-01
Recombinant adeno-associated viral (rAAV) vectors promote long-term gene transfer in many animal species. Significant effort has focused on the evaluation of rAAV delivery and the immune response in both murine and canine models of neuromuscular disease. However, canines provided for research purposes are routinely vaccinated ag...Read More
Matthew B Cooke, Paul La Bounty, Thomas Buford, Brian Shelmadine, Liz Redd, Geoffrey Hudson, Darryn S Willoughby
Journal of the International Society of Sports Nutrition 2011-01-01
This study examined the effects of a whey protein supplement in conjunction with an acute bout of lower body resistance exercise, in recreationally-active males, on serum insulin and insulin like growth factor 1 (IGF-1) and Akt/mTOR signaling markers indicative of muscle protein synthesis: insulin receptor substrate 1 (IRS-1), A...Read More
Aishe Chen, Lixue Dong, Nancy R Leffler, Adam S Asch, Owen N Witte, Li V Yang
PLoS ONE 2011-01-01
Endothelium-leukocyte interaction is critical for inflammatory responses. Whereas the tissue microenvironments are often acidic at inflammatory sites, the mechanisms by which cells respond to acidosis are not well understood. Using molecular, cellular and biochemical approaches, we demonstrate that activation of GPR4, a proton-s...Read More
Bart Bryant, Alexander S Raikhel
PLoS ONE 2011-01-01
Autophagy plays a pivotal role by allowing cells to recycle cellular components under conditions of stress, starvation, development and cancer. In this work, we have demonstrated that programmed autophagy in the mosquito fat body plays a critical role in maintaining of developmental switches required for normal progression of go...Read More
Fernanda R Roque, Ursula Paula Renó Soci, Katia De Angelis, Marcele A Coelho, Cristina R Furstenau, Dalton V Vassallo, Maria Claudia Irigoyen, Edilamar M Oliveira
Clinics 2011-01-01
Aerobic exercise training prevents cardiovascular risks. Regular exercise promotes functional and structural adaptations that are associated with several cardiovascular benefits. The aim of this study is to investigate the effects of swimming training on coronary blood flow, adenosine production and cardiac capillaries in normot...Read More
Sebastian Kobold, Sinje Tams, Tim Luetkens, Yanran Cao, Orhan Sezer, Britta Marlen Bartels, Henrike Reinhard, Julia Templin, Katrin Bartels, York Hildebrandt, Nesrine Lajmi, Andreas Marx, Friedrich Haag, Carsten Bokemeyer, Nicolaus Kröger, Djordje Atanackovic
Clinical and Developmental Immunology 2011-01-01
The occurrence of SOX2-specific autoantibodies seems to be associated with an improved prognosis in patients with monoclonal gammopathy of undetermined significance (MGUS). However, it is unclear if SOX2-specific antibodies also develop in established multiple myeloma (MM). Screening 1094 peripheral blood (PB) sera from 196 MM p...Read More
Jeffrey R Sommer, Venkata R M Chavali, Sean G Simpson, Radha Ayyagari, Robert M Petters
Molecular Vision 2012-01-01
Autosomal dominant early-onset long anterior zonules (LAZs) and late-onset retinal degeneration (L-ORD) in humans are associated with the S163R mutation of the complement 1q-tumor necrosis factor related protein-5 (CTRP5) gene. For using the pig as an L-ORD model for the study of pathology, we cloned, characterized, and studied ...Read More
Amy Huei-Yi Lee, Brenden Hurley, Corinna Felsensteiner, Carmen Yea, Wenzislava Ckurshumova, Verena Bartetzko, Pauline W Wang, Van Quach, Jennifer D Lewis, Yulu C Liu, Frederik Börnke, Stephane Angers, Andrew Wilde, David S Guttman, Darrell Desveaux
PLoS Pathogens 2012-02-01
The eukaryotic cytoskeleton is essential for structural support and intracellular transport, and is therefore a common target of animal pathogens. However, no phytopathogenic effector has yet been demonstrated to specifically target the plant cytoskeleton. Here we show that the Pseudomonas syringae type III secreted effector Hop...Read More
Ngoc-Duc Doan, David Chatenet, Myriam Létourneau, Hubert Vaudry, David Vaudry, Alain Fournier
Biochimica et Biophysica Acta 2012-04-01
Pituitary adenylate cyclase-activating polypeptide (PACAP), a hypophysiotropic neurohormone, participates in the regulation of pleiotropic functions. The recent discovery of intracellular PACAP receptors in the brain and the testis as well as the physico-chemical characteristics of PACAP, i.e. extended a-helix containing basic r...Read More
Elodie Bégué, Francette Jean-Louis, Martine Bagot, Sébastien Jauliac, Jean-Michel Cayuela, Liliane Laroche, Nathalie Parquet, Hervé Bachelez, Armand Bensussan, Gilles Courtois, Laurence Michel
Blood 2012-07-05
A molecular feature of Sézary syndrome (SS) is the abnormal expression of T-plastin by malignant T cells. Herein, we investigated the molecular mechanisms involved in T-plastin synthesis and the functions of this actin-binding protein, with a special interest in chemoresistance and migration. We confirm the specific expression o...Read More
Ifat Cohen-Or, Chen Katz, Eliora Z Ron
PLoS ONE 2013-01-01
Advanced Glycation End Products (AGEs) are the final products of non-enzymatic protein glycation that results in loss of protein structure and function. We have previously shown that in E. coli AGEs are continually formed as high-molecular weight protein complexes. Moreover, we showed that AGEs are removed from the cells by an a...Read More
Renzo Mancuso, Jaume Del Valle, Marta Morell, Mercé Pallás, Rosario Osta, Xavier Navarro
Orphanet Journal of Rare Diseases 2014-01-01
Amyotrophic lateral sclerosis (ALS) is an adult onset neurodegenerative disease characterized by the loss of motoneurons (MNs) in the spinal cord, brainstem and motor cortex, causing progressive paralysis and death. Nowadays, there is no effective therapy and most patients die 2-5 years after diagnosis. Sigma-1R is a transmembra...Read More