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Recombinant Rabbit Monoclonal Antibodies for Immunohistochemistry Applications

Sigma-Aldrich provides most characterized recombinant rabbit monoclonal antibodies for IHC applications in biomedical researches. The following three antibodies identify common cancer biomarkers.

  • Ki-67, Cell proliferation marker Anti-Ki 67 rabbit monoclonal antibody, clone RM227 (Product No. SAB5600050)
  • BRAF V600E, Detection of BRAF V600E mutation in melanoma and thyroid carcinoma Anti-BRAF (V600E) rabbit monoclonal antibody, clone RM8 (Product No. SAB5600047)
  • Histone H3 K36M, Chondroblastoma, bone cancer marker Anti-Histone H3 K36M rabbit monoclonal antibody, clone RM193 (Product SAB5600048)

 

Cell Proliferation Marker:
Anti-Ki67 rabbit monoclonal antibody, clone RM227 (Product No. SAB5600050)

Sigma-Aldrich Ki-67 specific rabbit monoclonal antibody outperforms competitor in identifying Ki-67 signals.

Competitor 1

competitor 1 tonsil

 

competitor 1 breast carcinoma

Sigma-Aldrich Clone

Sigma Aldrich clone tonsil

 

Sigma Aldrich breast carcinoma

 

BRAF V600E Mutation Detection in Thyroid Carcinoma
Anti-BRAF (V600E) rabbit monoclonal antibody, clone RM8 (cat no. SAB5600047)

Western blot analysis and immunohistochemical staining

Western Blot Analysis and Immunohistochemical staining of formalin fixed and paraffin embedded Thyroid Carcinoma tissue


A. Western Blot analysis of cell lysates prepared from cell lines expressing endogenous wild type (WT) or mutant (V600E) BRAF protein.  

B. Allele specific PCR validated to be Negative (left) and Positive (right) for the BRAF V600E Mutation.

 

Chondroblastoma, bone cancer marker:
Anti-Histone H3 K36M rabbit monoclonal antibody, clone RM193  (Product No. SAB5600048)

This antibody was applied to test over 1000 patients samples and showed highly specificity to the K36M mutation with indications in chondroblastoma.

Formalin-fixed paraffin-embedded HEK 293 cells expressing Histone K36M

A

Formalin-fixed paraffin-embedded HEK 293 cells expressing SK-MEL-2

B

Immunohistochemical Staining
Formalin-fixed paraffin-embedded HEK 293 cells expressing Histone K36M (A) or SK-MEL-2 (B) were  stained with Sigma-Aldrich Histone K36M Specific Rabbit Monoclonal Antibody.

 

Sigma-Aldrich also provides other high quality recombinant rabbit monoclonal antibodies for IHC applications.

Description Product No.
Anti-Cytokeratin 5 rabbit monoclonal antibody [RM226] SAB5600049
Anti-HER2 rabbit monoclonal antibody [RM228] SAB5600051
Anti-Desmin (DESM) rabbit monoclonal antibody [RM234] SAB5600054
Anti-Cyclin D1 (CCND1) rabbit monoclonal antibody [RM241] SAB5600055
Anti-HIF-1 alpha (HIF1A) rabbit monoclonal antibody [RM242] SAB5600056
Anti-E-Cadherin (CDH1) rabbit monoclonal antibody [RM244] SAB5600058
Anti-GFAP rabbit monoclonal antibody [RM246] SAB5600060
Anti-CD31 (PECAM-1) rabbit monoclonal antibody [RM247] SAB5600061
Anti-CD146 rabbit monoclonal antibody [RM249] SAB5600062
Anti-Caspase-3 rabbit monoclonal antibody [RM250] SAB5600063
Anti-ACTA2 rabbit monoclonal antibody [RM253] SAB5600065
Anti-Akt1 rabbit monoclonal antibody [RM252] SAB5600066
Anti-AR (N-terminal) rabbit monoclonal antibody [RM254] SAB5600067
Anti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal Antibody [RM257] SAB5600071
Anti-Synaptophysin Rabbit Monoclonal Antibody [RM258] SAB5600073
Anti-CD44 Rabbit Monoclonal Antibody [RM264] SAB5600074
Anti-PTEN Rabbit Monoclonal Antibody [RM265] SAB5600075
Anti-CK8 (Cytokeratin-8) Rabbit Monoclonal Antibody [RM266] SAB5600076
Anti-p16INK4a (CDKN2A) Rabbit Monoclonal Antibody [RM267] SAB5600077
Anti-Integrin alpha 4 (ITGA4) Rabbit Monoclonal Antibody [RM268] SAB5600078
Anti-Smac/Diablo Rabbit Monoclonal Antibody [RM271] SAB5600081
Anti-CD20 Rabbit Monoclonal Antibody [RM272] SAB5600082
Anti-NFkB p65 Rabbit Monoclonal Antibody [RM273] SAB5600083
Anti-mTOR Rabbit Monoclonal Antibody [RM274] SAB5600084
Anti-JAM1 Rabbit Monoclonal Antibody [RM275] SAB5600085
Anti-Beta-Catenin 1 (CTNNB1) Rabbit Monoclonal Antibody [RM276] SAB5600086
Anti-Smad4 Rabbit Monoclonal Antibody [RM277] SAB5600087
Anti-Aurora-B (AURKB) Rabbit Monoclonal Antibody [RM278] SAB5600088
Anti-CK18 Rabbit Monoclonal Antibody [RM279] SAB5600089
Anti-Neurofilament-L (NF-L) Rabbit Monoclonal Antibody [RM280] SAB5600090
Anti-Cyclin B1 Rabbit Monoclonal Antibody [RM281] SAB5600091

Materials

     
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